Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23855 and EX31_RS23860 are separated by 335 nucleotides EX31_RS23860 and EX31_RS23865 are separated by 58 nucleotides
EX31_RS23855: EX31_RS23855 - NAD-dependent succinate-semialdehyde dehydrogenase, at 539,194 to 540,573
_RS23855
EX31_RS23860: EX31_RS23860 - hypothetical protein, at 540,909 to 541,238
_RS23860
EX31_RS23865: EX31_RS23865 - D-alanine--D-alanine ligase, at 541,297 to 542,394
_RS23865
Position (kb) 540
541
542 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 540.018 kb on + strand, within EX31_RS23855 at 540.018 kb on + strand, within EX31_RS23855 at 540.030 kb on + strand, within EX31_RS23855 at 540.030 kb on + strand, within EX31_RS23855 at 540.030 kb on + strand, within EX31_RS23855 at 540.030 kb on + strand, within EX31_RS23855 at 540.031 kb on - strand, within EX31_RS23855 at 540.031 kb on - strand, within EX31_RS23855 at 540.031 kb on - strand, within EX31_RS23855 at 540.031 kb on - strand, within EX31_RS23855 at 540.127 kb on - strand, within EX31_RS23855 at 540.152 kb on - strand, within EX31_RS23855 at 540.161 kb on - strand, within EX31_RS23855 at 540.249 kb on + strand, within EX31_RS23855 at 540.249 kb on + strand, within EX31_RS23855 at 540.250 kb on - strand, within EX31_RS23855 at 540.358 kb on + strand, within EX31_RS23855 at 540.358 kb on + strand, within EX31_RS23855 at 540.359 kb on - strand, within EX31_RS23855 at 540.414 kb on + strand, within EX31_RS23855 at 540.414 kb on + strand, within EX31_RS23855 at 540.414 kb on + strand, within EX31_RS23855 at 540.415 kb on - strand, within EX31_RS23855 at 540.415 kb on - strand, within EX31_RS23855 at 540.415 kb on - strand, within EX31_RS23855 at 540.415 kb on - strand, within EX31_RS23855 at 540.415 kb on - strand, within EX31_RS23855 at 540.439 kb on - strand at 540.442 kb on - strand at 540.442 kb on - strand at 540.468 kb on - strand at 540.470 kb on - strand at 540.513 kb on + strand at 540.514 kb on - strand at 540.540 kb on + strand at 540.541 kb on - strand at 540.557 kb on - strand at 540.557 kb on - strand at 540.557 kb on - strand at 540.573 kb on + strand at 540.573 kb on + strand at 540.573 kb on + strand at 540.574 kb on - strand at 540.574 kb on - strand at 540.574 kb on - strand at 540.574 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.665 kb on - strand at 540.670 kb on + strand at 540.671 kb on - strand at 540.671 kb on - strand at 540.762 kb on - strand at 540.762 kb on - strand at 540.762 kb on - strand at 540.823 kb on - strand at 540.869 kb on + strand at 540.870 kb on - strand at 541.001 kb on + strand, within EX31_RS23860 at 541.041 kb on + strand, within EX31_RS23860 at 541.042 kb on - strand, within EX31_RS23860 at 541.042 kb on - strand, within EX31_RS23860 at 541.076 kb on + strand, within EX31_RS23860 at 541.077 kb on - strand, within EX31_RS23860 at 541.165 kb on - strand, within EX31_RS23860 at 541.237 kb on - strand at 541.302 kb on + strand at 541.318 kb on - strand at 541.318 kb on - strand at 541.321 kb on - strand at 541.446 kb on + strand, within EX31_RS23865 at 541.449 kb on + strand, within EX31_RS23865 at 541.449 kb on + strand, within EX31_RS23865 at 541.461 kb on + strand, within EX31_RS23865 at 541.556 kb on + strand, within EX31_RS23865 at 541.557 kb on - strand, within EX31_RS23865 at 541.668 kb on + strand, within EX31_RS23865 at 541.845 kb on + strand, within EX31_RS23865 at 541.884 kb on + strand, within EX31_RS23865 at 541.884 kb on + strand, within EX31_RS23865 at 542.062 kb on + strand, within EX31_RS23865 at 542.063 kb on - strand, within EX31_RS23865 at 542.074 kb on + strand, within EX31_RS23865 at 542.074 kb on + strand, within EX31_RS23865 at 542.074 kb on + strand, within EX31_RS23865 at 542.074 kb on + strand, within EX31_RS23865 at 542.074 kb on + strand, within EX31_RS23865 at 542.075 kb on - strand, within EX31_RS23865
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 540,018 + EX31_RS23855 0.60 +0.5 540,018 + EX31_RS23855 0.60 +0.2 540,030 + EX31_RS23855 0.61 -1.1 540,030 + EX31_RS23855 0.61 -3.3 540,030 + EX31_RS23855 0.61 +0.7 540,030 + EX31_RS23855 0.61 -0.0 540,031 - EX31_RS23855 0.61 -0.3 540,031 - EX31_RS23855 0.61 -3.1 540,031 - EX31_RS23855 0.61 -0.0 540,031 - EX31_RS23855 0.61 -0.1 540,127 - EX31_RS23855 0.68 -0.0 540,152 - EX31_RS23855 0.69 -1.4 540,161 - EX31_RS23855 0.70 +2.2 540,249 + EX31_RS23855 0.76 -0.9 540,249 + EX31_RS23855 0.76 +0.2 540,250 - EX31_RS23855 0.77 -0.9 540,358 + EX31_RS23855 0.84 -1.4 540,358 + EX31_RS23855 0.84 -0.2 540,359 - EX31_RS23855 0.84 -0.3 540,414 + EX31_RS23855 0.88 +1.2 540,414 + EX31_RS23855 0.88 -0.3 540,414 + EX31_RS23855 0.88 +1.1 540,415 - EX31_RS23855 0.88 -0.2 540,415 - EX31_RS23855 0.88 -0.0 540,415 - EX31_RS23855 0.88 +1.4 540,415 - EX31_RS23855 0.88 -1.3 540,415 - EX31_RS23855 0.88 +1.0 540,439 - -1.1 540,442 - -2.0 540,442 - +0.4 540,468 - -1.8 540,470 - -0.3 540,513 + -0.1 540,514 - -0.2 540,540 + -0.1 540,541 - +0.1 540,557 - +0.4 540,557 - +1.2 540,557 - -2.7 540,573 + -0.6 540,573 + +2.8 540,573 + +1.4 540,574 - -0.5 540,574 - +1.2 540,574 - +0.3 540,574 - +0.5 540,665 - +0.8 540,665 - -1.2 540,665 - +0.2 540,665 - -0.6 540,670 + +0.5 540,671 - +0.6 540,671 - -0.0 540,762 - +0.7 540,762 - -0.9 540,762 - +0.1 540,823 - -0.7 540,869 + -1.8 540,870 - -1.0 541,001 + EX31_RS23860 0.28 -0.5 541,041 + EX31_RS23860 0.40 -0.4 541,042 - EX31_RS23860 0.40 -0.1 541,042 - EX31_RS23860 0.40 -0.6 541,076 + EX31_RS23860 0.51 -2.4 541,077 - EX31_RS23860 0.51 +0.6 541,165 - EX31_RS23860 0.78 +0.1 541,237 - -0.6 541,302 + +1.1 541,318 - +0.1 541,318 - -1.1 541,321 - -0.1 541,446 + EX31_RS23865 0.14 -0.1 541,449 + EX31_RS23865 0.14 +0.4 541,449 + EX31_RS23865 0.14 +0.8 541,461 + EX31_RS23865 0.15 +0.7 541,556 + EX31_RS23865 0.24 -0.4 541,557 - EX31_RS23865 0.24 +0.7 541,668 + EX31_RS23865 0.34 +0.0 541,845 + EX31_RS23865 0.50 +0.0 541,884 + EX31_RS23865 0.53 -0.4 541,884 + EX31_RS23865 0.53 -2.1 542,062 + EX31_RS23865 0.70 +0.2 542,063 - EX31_RS23865 0.70 +0.3 542,074 + EX31_RS23865 0.71 -0.2 542,074 + EX31_RS23865 0.71 -0.6 542,074 + EX31_RS23865 0.71 -0.6 542,074 + EX31_RS23865 0.71 +1.2 542,074 + EX31_RS23865 0.71 -0.1 542,075 - EX31_RS23865 0.71 +0.2
Or see this region's nucleotide sequence