Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18820 and EX31_RS18825 are separated by 44 nucleotides EX31_RS18825 and EX31_RS18830 are separated by 236 nucleotides
EX31_RS18820: EX31_RS18820 - betaine-aldehyde dehydrogenase, at 348,205 to 349,674
_RS18820
EX31_RS18825: EX31_RS18825 - transcriptional regulator BetI, at 349,719 to 350,318
_RS18825
EX31_RS18830: EX31_RS18830 - choline transporter, at 350,555 to 352,609
_RS18830
Position (kb) 349
350
351 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.781 kb on - strand, within EX31_RS18820 at 348.799 kb on - strand, within EX31_RS18820 at 348.799 kb on - strand, within EX31_RS18820 at 348.873 kb on + strand, within EX31_RS18820 at 349.100 kb on + strand, within EX31_RS18820 at 349.101 kb on - strand, within EX31_RS18820 at 349.266 kb on - strand, within EX31_RS18820 at 349.328 kb on + strand, within EX31_RS18820 at 349.329 kb on - strand, within EX31_RS18820 at 349.329 kb on - strand, within EX31_RS18820 at 349.331 kb on + strand, within EX31_RS18820 at 349.332 kb on - strand, within EX31_RS18820 at 349.332 kb on - strand, within EX31_RS18820 at 349.594 kb on - strand at 349.645 kb on - strand at 349.645 kb on - strand at 349.647 kb on - strand at 349.647 kb on - strand at 349.647 kb on - strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.674 kb on + strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.675 kb on - strand at 349.758 kb on - strand at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.909 kb on - strand, within EX31_RS18825 at 349.919 kb on - strand, within EX31_RS18825 at 349.951 kb on + strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.139 kb on - strand, within EX31_RS18825 at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.278 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.308 kb on - strand at 350.371 kb on + strand at 350.371 kb on + strand at 350.372 kb on - strand at 350.393 kb on - strand at 350.393 kb on - strand at 350.393 kb on - strand at 350.413 kb on - strand at 350.455 kb on + strand at 350.455 kb on + strand at 350.456 kb on - strand at 350.456 kb on - strand at 350.470 kb on - strand at 350.470 kb on - strand at 350.470 kb on - strand at 350.478 kb on - strand at 350.532 kb on + strand at 350.532 kb on + strand at 350.541 kb on + strand at 350.542 kb on - strand at 350.738 kb on + strand at 350.739 kb on - strand at 350.741 kb on + strand at 350.741 kb on + strand at 350.741 kb on + strand at 350.741 kb on + strand at 350.741 kb on + strand at 350.741 kb on + strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.742 kb on - strand at 350.755 kb on + strand at 350.762 kb on + strand, within EX31_RS18830 at 350.762 kb on + strand, within EX31_RS18830 at 350.762 kb on + strand, within EX31_RS18830 at 350.762 kb on + strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.763 kb on - strand, within EX31_RS18830 at 350.775 kb on + strand, within EX31_RS18830 at 350.775 kb on + strand, within EX31_RS18830 at 350.775 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.930 kb on + strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 350.931 kb on - strand, within EX31_RS18830 at 351.029 kb on + strand, within EX31_RS18830 at 351.071 kb on + strand, within EX31_RS18830 at 351.071 kb on + strand, within EX31_RS18830 at 351.072 kb on - strand, within EX31_RS18830 at 351.072 kb on - strand, within EX31_RS18830 at 351.073 kb on + strand, within EX31_RS18830 at 351.074 kb on - strand, within EX31_RS18830 at 351.074 kb on - strand, within EX31_RS18830 at 351.145 kb on + strand, within EX31_RS18830 at 351.146 kb on - strand, within EX31_RS18830 at 351.175 kb on + strand, within EX31_RS18830 at 351.220 kb on + strand, within EX31_RS18830 at 351.221 kb on - strand, within EX31_RS18830 at 351.224 kb on + strand, within EX31_RS18830 at 351.224 kb on + strand, within EX31_RS18830 at 351.225 kb on - strand, within EX31_RS18830 at 351.247 kb on + strand, within EX31_RS18830 at 351.248 kb on - strand, within EX31_RS18830
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 348,781 - EX31_RS18820 0.39 -0.4 348,781 - EX31_RS18820 0.39 -0.7 348,781 - EX31_RS18820 0.39 +0.7 348,781 - EX31_RS18820 0.39 -0.0 348,799 - EX31_RS18820 0.40 +0.7 348,799 - EX31_RS18820 0.40 +0.5 348,873 + EX31_RS18820 0.45 -0.7 349,100 + EX31_RS18820 0.61 +0.6 349,101 - EX31_RS18820 0.61 -1.5 349,266 - EX31_RS18820 0.72 +0.4 349,328 + EX31_RS18820 0.76 -0.2 349,329 - EX31_RS18820 0.76 +0.8 349,329 - EX31_RS18820 0.76 +0.4 349,331 + EX31_RS18820 0.77 +1.4 349,332 - EX31_RS18820 0.77 +0.6 349,332 - EX31_RS18820 0.77 -0.1 349,594 - +0.7 349,645 - +1.1 349,645 - +1.7 349,647 - -1.0 349,647 - +0.1 349,647 - -0.9 349,674 + -0.4 349,674 + +0.7 349,674 + +0.4 349,674 + -0.5 349,674 + +1.5 349,674 + -0.6 349,674 + -0.0 349,674 + -2.0 349,674 + -1.1 349,674 + +2.1 349,674 + +1.5 349,674 + -0.3 349,674 + -0.1 349,674 + +1.1 349,674 + +1.3 349,674 + +0.1 349,675 - -0.3 349,675 - -0.3 349,675 - +0.1 349,675 - +0.4 349,675 - -0.1 349,675 - +0.1 349,675 - +0.2 349,675 - -1.1 349,675 - +0.0 349,675 - -0.4 349,675 - -3.8 349,675 - +0.4 349,675 - +1.0 349,675 - +0.1 349,675 - -0.2 349,675 - -1.7 349,675 - -1.1 349,675 - -0.5 349,675 - +0.2 349,675 - +0.1 349,758 - +0.6 349,909 - EX31_RS18825 0.32 -0.1 349,909 - EX31_RS18825 0.32 -0.3 349,909 - EX31_RS18825 0.32 -1.8 349,909 - EX31_RS18825 0.32 -0.1 349,919 - EX31_RS18825 0.33 -1.9 349,951 + EX31_RS18825 0.39 +1.0 350,139 - EX31_RS18825 0.70 -2.1 350,139 - EX31_RS18825 0.70 +0.6 350,139 - EX31_RS18825 0.70 -0.8 350,139 - EX31_RS18825 0.70 -0.2 350,278 - +0.0 350,278 - +0.3 350,278 - -0.7 350,278 - +0.5 350,278 - -0.8 350,308 - -0.3 350,308 - +0.0 350,308 - +0.0 350,308 - +0.4 350,371 + +0.5 350,371 + +0.3 350,372 - -0.4 350,393 - +0.2 350,393 - +0.4 350,393 - -0.6 350,413 - +0.1 350,455 + +0.4 350,455 + +0.7 350,456 - -0.3 350,456 - +0.3 350,470 - -0.0 350,470 - -0.5 350,470 - -0.0 350,478 - +0.6 350,532 + -0.1 350,532 + +0.8 350,541 + -0.2 350,542 - +2.2 350,738 + +0.8 350,739 - -0.0 350,741 + +0.4 350,741 + +0.0 350,741 + +0.7 350,741 + +1.6 350,741 + -2.3 350,741 + +0.9 350,742 - +0.2 350,742 - -0.4 350,742 - +1.2 350,742 - -0.6 350,742 - +0.1 350,742 - -0.4 350,742 - -0.3 350,742 - -0.6 350,742 - +0.6 350,742 - +0.1 350,755 + -0.9 350,762 + EX31_RS18830 0.10 -0.4 350,762 + EX31_RS18830 0.10 +0.0 350,762 + EX31_RS18830 0.10 -0.8 350,762 + EX31_RS18830 0.10 -2.0 350,763 - EX31_RS18830 0.10 +1.2 350,763 - EX31_RS18830 0.10 -0.8 350,763 - EX31_RS18830 0.10 -3.0 350,763 - EX31_RS18830 0.10 -0.5 350,763 - EX31_RS18830 0.10 -0.2 350,763 - EX31_RS18830 0.10 +0.0 350,763 - EX31_RS18830 0.10 +1.4 350,763 - EX31_RS18830 0.10 +0.0 350,775 + EX31_RS18830 0.11 -0.3 350,775 + EX31_RS18830 0.11 +0.1 350,775 + EX31_RS18830 0.11 -0.1 350,930 + EX31_RS18830 0.18 +1.1 350,930 + EX31_RS18830 0.18 -0.6 350,930 + EX31_RS18830 0.18 +0.2 350,930 + EX31_RS18830 0.18 +0.2 350,930 + EX31_RS18830 0.18 +0.8 350,930 + EX31_RS18830 0.18 -0.5 350,930 + EX31_RS18830 0.18 +0.3 350,930 + EX31_RS18830 0.18 -0.7 350,930 + EX31_RS18830 0.18 +0.3 350,930 + EX31_RS18830 0.18 -0.4 350,930 + EX31_RS18830 0.18 -1.9 350,930 + EX31_RS18830 0.18 +0.2 350,930 + EX31_RS18830 0.18 +0.3 350,930 + EX31_RS18830 0.18 -0.2 350,930 + EX31_RS18830 0.18 +0.2 350,930 + EX31_RS18830 0.18 -0.5 350,930 + EX31_RS18830 0.18 +0.6 350,930 + EX31_RS18830 0.18 -0.3 350,931 - EX31_RS18830 0.18 +0.3 350,931 - EX31_RS18830 0.18 +0.5 350,931 - EX31_RS18830 0.18 -0.4 350,931 - EX31_RS18830 0.18 -0.4 350,931 - EX31_RS18830 0.18 -0.8 350,931 - EX31_RS18830 0.18 -1.0 350,931 - EX31_RS18830 0.18 -0.1 350,931 - EX31_RS18830 0.18 -0.3 350,931 - EX31_RS18830 0.18 -0.4 350,931 - EX31_RS18830 0.18 -0.5 350,931 - EX31_RS18830 0.18 -2.0 350,931 - EX31_RS18830 0.18 +0.8 350,931 - EX31_RS18830 0.18 +0.3 350,931 - EX31_RS18830 0.18 +0.3 350,931 - EX31_RS18830 0.18 -0.5 351,029 + EX31_RS18830 0.23 +0.1 351,071 + EX31_RS18830 0.25 +0.4 351,071 + EX31_RS18830 0.25 +1.4 351,072 - EX31_RS18830 0.25 -0.1 351,072 - EX31_RS18830 0.25 -0.6 351,073 + EX31_RS18830 0.25 +0.1 351,074 - EX31_RS18830 0.25 +0.3 351,074 - EX31_RS18830 0.25 +0.4 351,145 + EX31_RS18830 0.29 +0.1 351,146 - EX31_RS18830 0.29 +0.8 351,175 + EX31_RS18830 0.30 +0.1 351,220 + EX31_RS18830 0.32 +0.3 351,221 - EX31_RS18830 0.32 -0.1 351,224 + EX31_RS18830 0.33 +0.0 351,224 + EX31_RS18830 0.33 +0.7 351,225 - EX31_RS18830 0.33 +0.3 351,247 + EX31_RS18830 0.34 +1.0 351,248 - EX31_RS18830 0.34 +0.8
Or see this region's nucleotide sequence