Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23655 and EX31_RS23660 are separated by 37 nucleotides EX31_RS23660 and EX31_RS23665 are separated by 41 nucleotides EX31_RS23665 and EX31_RS23670 are separated by 271 nucleotides
EX31_RS23655: EX31_RS23655 - kinase inhibitor, at 495,966 to 496,448
_RS23655
EX31_RS23660: EX31_RS23660 - type 1 glutamine amidotransferase domain-containing protein, at 496,486 to 497,181
_RS23660
EX31_RS23665: EX31_RS23665 - NADP-dependent oxidoreductase, at 497,223 to 498,260
_RS23665
EX31_RS23670: EX31_RS23670 - acid phosphatase AphA, at 498,532 to 499,245
_RS23670
Position (kb) 497
498
499 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 496.238 kb on + strand, within EX31_RS23655 at 496.291 kb on + strand, within EX31_RS23655 at 496.369 kb on + strand, within EX31_RS23655 at 496.427 kb on + strand at 496.439 kb on + strand at 496.439 kb on + strand at 496.440 kb on - strand at 496.440 kb on - strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.448 kb on + strand at 496.449 kb on - strand at 496.449 kb on - strand at 496.449 kb on - strand at 496.488 kb on - strand at 496.488 kb on - strand at 496.488 kb on - strand at 496.523 kb on - strand at 496.523 kb on - strand at 496.614 kb on + strand, within EX31_RS23660 at 496.614 kb on + strand, within EX31_RS23660 at 496.615 kb on - strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.701 kb on + strand, within EX31_RS23660 at 496.702 kb on - strand, within EX31_RS23660 at 496.704 kb on + strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.705 kb on - strand, within EX31_RS23660 at 496.962 kb on + strand, within EX31_RS23660 at 496.963 kb on - strand, within EX31_RS23660 at 496.963 kb on - strand, within EX31_RS23660 at 496.963 kb on - strand, within EX31_RS23660 at 497.087 kb on + strand, within EX31_RS23660 at 497.088 kb on - strand, within EX31_RS23660 at 497.149 kb on - strand at 497.149 kb on - strand at 497.164 kb on + strand at 497.165 kb on - strand at 497.174 kb on - strand at 497.209 kb on + strand at 497.210 kb on - strand at 497.210 kb on - strand at 497.210 kb on - strand at 497.221 kb on - strand at 497.395 kb on + strand, within EX31_RS23665 at 497.396 kb on - strand, within EX31_RS23665 at 497.409 kb on - strand, within EX31_RS23665 at 497.498 kb on + strand, within EX31_RS23665 at 497.499 kb on - strand, within EX31_RS23665 at 497.499 kb on - strand, within EX31_RS23665 at 497.499 kb on - strand, within EX31_RS23665 at 497.499 kb on - strand, within EX31_RS23665 at 497.529 kb on - strand, within EX31_RS23665 at 497.594 kb on - strand, within EX31_RS23665 at 497.594 kb on - strand, within EX31_RS23665 at 497.594 kb on - strand, within EX31_RS23665 at 497.748 kb on - strand, within EX31_RS23665 at 497.843 kb on + strand, within EX31_RS23665 at 497.868 kb on + strand, within EX31_RS23665 at 497.868 kb on + strand, within EX31_RS23665 at 497.868 kb on + strand, within EX31_RS23665 at 497.948 kb on - strand, within EX31_RS23665 at 497.948 kb on - strand, within EX31_RS23665 at 498.042 kb on + strand, within EX31_RS23665 at 498.043 kb on - strand, within EX31_RS23665 at 498.043 kb on - strand, within EX31_RS23665 at 498.070 kb on + strand, within EX31_RS23665 at 498.071 kb on - strand, within EX31_RS23665 at 498.071 kb on - strand, within EX31_RS23665 at 498.089 kb on + strand, within EX31_RS23665 at 498.089 kb on + strand, within EX31_RS23665 at 498.089 kb on + strand, within EX31_RS23665 at 498.090 kb on - strand, within EX31_RS23665 at 498.090 kb on - strand, within EX31_RS23665 at 498.090 kb on - strand, within EX31_RS23665 at 498.128 kb on + strand, within EX31_RS23665 at 498.128 kb on + strand, within EX31_RS23665 at 498.138 kb on + strand, within EX31_RS23665 at 498.138 kb on + strand, within EX31_RS23665 at 498.139 kb on - strand, within EX31_RS23665 at 498.139 kb on - strand, within EX31_RS23665 at 498.139 kb on - strand, within EX31_RS23665 at 498.260 kb on + strand at 498.260 kb on + strand at 498.260 kb on + strand at 498.261 kb on - strand at 498.285 kb on - strand at 498.305 kb on + strand at 498.306 kb on - strand at 498.306 kb on - strand at 498.306 kb on - strand at 498.306 kb on - strand at 498.306 kb on - strand at 498.316 kb on - strand at 498.319 kb on + strand at 498.319 kb on + strand at 498.319 kb on + strand at 498.319 kb on + strand at 498.320 kb on - strand at 498.321 kb on + strand at 498.321 kb on + strand at 498.322 kb on - strand at 498.322 kb on - strand at 498.329 kb on + strand at 498.354 kb on + strand at 498.354 kb on + strand at 498.413 kb on + strand at 498.414 kb on - strand at 498.459 kb on + strand at 498.469 kb on - strand at 498.496 kb on - strand at 498.507 kb on + strand at 498.508 kb on - strand at 498.510 kb on + strand at 498.517 kb on - strand at 498.517 kb on - strand at 498.517 kb on - strand at 498.530 kb on + strand at 498.530 kb on + strand at 498.530 kb on + strand at 498.537 kb on + strand at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.609 kb on + strand, within EX31_RS23670 at 498.610 kb on - strand, within EX31_RS23670 at 498.722 kb on + strand, within EX31_RS23670 at 498.722 kb on + strand, within EX31_RS23670 at 498.723 kb on - strand, within EX31_RS23670 at 498.723 kb on - strand, within EX31_RS23670 at 498.723 kb on - strand, within EX31_RS23670 at 498.814 kb on + strand, within EX31_RS23670 at 498.815 kb on - strand, within EX31_RS23670 at 498.931 kb on + strand, within EX31_RS23670 at 498.932 kb on - strand, within EX31_RS23670 at 498.932 kb on - strand, within EX31_RS23670 at 499.119 kb on + strand, within EX31_RS23670 at 499.119 kb on + strand, within EX31_RS23670 at 499.119 kb on + strand, within EX31_RS23670 at 499.120 kb on - strand, within EX31_RS23670 at 499.120 kb on - strand, within EX31_RS23670 at 499.120 kb on - strand, within EX31_RS23670 at 499.120 kb on - strand, within EX31_RS23670 at 499.120 kb on - strand, within EX31_RS23670 at 499.161 kb on - strand, within EX31_RS23670 at 499.173 kb on + strand, within EX31_RS23670 at 499.183 kb on + strand at 499.183 kb on + strand at 499.183 kb on + strand at 499.183 kb on + strand at 499.241 kb on - strand at 499.241 kb on - strand at 499.243 kb on + strand at 499.243 kb on + strand at 499.243 kb on + strand at 499.243 kb on + strand at 499.243 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 496,238 + EX31_RS23655 0.56 -0.7 496,291 + EX31_RS23655 0.67 -0.8 496,369 + EX31_RS23655 0.83 -0.1 496,427 + +0.3 496,439 + +1.2 496,439 + -0.9 496,440 - -0.5 496,440 - +0.5 496,448 + -0.4 496,448 + +0.2 496,448 + +0.0 496,448 + -1.8 496,449 - -0.2 496,449 - +0.6 496,449 - +0.8 496,488 - -1.3 496,488 - +0.6 496,488 - +1.6 496,523 - -1.8 496,523 - -0.2 496,614 + EX31_RS23660 0.18 -0.1 496,614 + EX31_RS23660 0.18 -0.4 496,615 - EX31_RS23660 0.19 -2.1 496,701 + EX31_RS23660 0.31 -0.4 496,701 + EX31_RS23660 0.31 -0.1 496,701 + EX31_RS23660 0.31 -1.2 496,702 - EX31_RS23660 0.31 +1.0 496,704 + EX31_RS23660 0.31 +0.8 496,705 - EX31_RS23660 0.31 +0.2 496,705 - EX31_RS23660 0.31 -0.5 496,705 - EX31_RS23660 0.31 +0.4 496,705 - EX31_RS23660 0.31 -0.3 496,962 + EX31_RS23660 0.68 +0.3 496,963 - EX31_RS23660 0.69 -0.6 496,963 - EX31_RS23660 0.69 -0.8 496,963 - EX31_RS23660 0.69 -1.1 497,087 + EX31_RS23660 0.86 -0.5 497,088 - EX31_RS23660 0.86 +1.0 497,149 - -0.4 497,149 - +0.0 497,164 + -0.2 497,165 - +0.5 497,174 - -2.2 497,209 + -1.9 497,210 - -0.3 497,210 - +0.4 497,210 - -0.1 497,221 - -0.1 497,395 + EX31_RS23665 0.17 +0.1 497,396 - EX31_RS23665 0.17 +0.1 497,409 - EX31_RS23665 0.18 -0.9 497,498 + EX31_RS23665 0.26 -0.1 497,499 - EX31_RS23665 0.27 +0.3 497,499 - EX31_RS23665 0.27 -0.4 497,499 - EX31_RS23665 0.27 -0.7 497,499 - EX31_RS23665 0.27 -2.6 497,529 - EX31_RS23665 0.29 -0.0 497,594 - EX31_RS23665 0.36 -1.0 497,594 - EX31_RS23665 0.36 -0.3 497,594 - EX31_RS23665 0.36 -1.3 497,748 - EX31_RS23665 0.51 -1.1 497,843 + EX31_RS23665 0.60 -1.6 497,868 + EX31_RS23665 0.62 +0.6 497,868 + EX31_RS23665 0.62 -0.9 497,868 + EX31_RS23665 0.62 -0.4 497,948 - EX31_RS23665 0.70 -0.4 497,948 - EX31_RS23665 0.70 -0.4 498,042 + EX31_RS23665 0.79 -0.9 498,043 - EX31_RS23665 0.79 +0.6 498,043 - EX31_RS23665 0.79 -0.2 498,070 + EX31_RS23665 0.82 -1.7 498,071 - EX31_RS23665 0.82 -0.3 498,071 - EX31_RS23665 0.82 -0.1 498,089 + EX31_RS23665 0.83 +0.8 498,089 + EX31_RS23665 0.83 -0.4 498,089 + EX31_RS23665 0.83 -1.0 498,090 - EX31_RS23665 0.84 -2.3 498,090 - EX31_RS23665 0.84 -0.4 498,090 - EX31_RS23665 0.84 -0.5 498,128 + EX31_RS23665 0.87 -0.5 498,128 + EX31_RS23665 0.87 +0.1 498,138 + EX31_RS23665 0.88 -0.0 498,138 + EX31_RS23665 0.88 -0.3 498,139 - EX31_RS23665 0.88 -0.5 498,139 - EX31_RS23665 0.88 +0.1 498,139 - EX31_RS23665 0.88 -1.4 498,260 + +0.6 498,260 + +0.0 498,260 + -0.8 498,261 - -0.5 498,285 - +0.8 498,305 + -1.2 498,306 - -0.5 498,306 - -1.2 498,306 - -0.1 498,306 - +0.8 498,306 - +0.8 498,316 - +1.0 498,319 + +1.1 498,319 + +1.2 498,319 + +0.2 498,319 + -0.3 498,320 - +0.5 498,321 + -2.2 498,321 + -1.0 498,322 - -0.4 498,322 - -0.5 498,329 + +0.8 498,354 + -0.2 498,354 + +0.3 498,413 + -0.2 498,414 - -0.5 498,459 + +0.0 498,469 - +0.2 498,496 - +0.1 498,507 + +0.1 498,508 - +0.1 498,510 + -0.6 498,517 - +0.5 498,517 - -0.1 498,517 - +0.4 498,530 + -0.0 498,530 + +0.8 498,530 + -0.4 498,537 + -0.5 498,609 + EX31_RS23670 0.11 -0.6 498,609 + EX31_RS23670 0.11 +0.6 498,609 + EX31_RS23670 0.11 -0.2 498,609 + EX31_RS23670 0.11 -3.4 498,609 + EX31_RS23670 0.11 +0.2 498,609 + EX31_RS23670 0.11 -0.7 498,609 + EX31_RS23670 0.11 +0.1 498,609 + EX31_RS23670 0.11 -0.6 498,610 - EX31_RS23670 0.11 -1.5 498,722 + EX31_RS23670 0.27 +2.2 498,722 + EX31_RS23670 0.27 +0.8 498,723 - EX31_RS23670 0.27 +1.5 498,723 - EX31_RS23670 0.27 -0.0 498,723 - EX31_RS23670 0.27 +0.7 498,814 + EX31_RS23670 0.39 -0.4 498,815 - EX31_RS23670 0.40 -1.7 498,931 + EX31_RS23670 0.56 -0.5 498,932 - EX31_RS23670 0.56 +0.1 498,932 - EX31_RS23670 0.56 -1.5 499,119 + EX31_RS23670 0.82 +0.5 499,119 + EX31_RS23670 0.82 -0.1 499,119 + EX31_RS23670 0.82 +0.7 499,120 - EX31_RS23670 0.82 -0.2 499,120 - EX31_RS23670 0.82 +2.0 499,120 - EX31_RS23670 0.82 +1.0 499,120 - EX31_RS23670 0.82 +0.4 499,120 - EX31_RS23670 0.82 -0.0 499,161 - EX31_RS23670 0.88 +0.0 499,173 + EX31_RS23670 0.90 +1.2 499,183 + -0.5 499,183 + -0.7 499,183 + -1.4 499,183 + +0.8 499,241 - -0.3 499,241 - -0.8 499,243 + -1.4 499,243 + -0.1 499,243 + +0.1 499,243 + +0.3 499,243 + +0.4
Or see this region's nucleotide sequence