Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS19745 and EX31_RS19750 overlap by 4 nucleotides EX31_RS19750 and EX31_RS19755 overlap by 4 nucleotides EX31_RS19755 and EX31_RS19760 overlap by 4 nucleotides EX31_RS19760 and EX31_RS19765 are separated by 217 nucleotides EX31_RS19765 and EX31_RS19770 are separated by 21 nucleotides
EX31_RS19745: EX31_RS19745 - heme lyase CcmF/NrfE family subunit, at 582,043 to 584,016
_RS19745
EX31_RS19750: EX31_RS19750 - cytochrome c maturation protein CcmE, at 584,013 to 584,507
_RS19750
EX31_RS19755: EX31_RS19755 - heme exporter protein CcmD, at 584,504 to 584,764
_RS19755
EX31_RS19760: EX31_RS19760 - heme ABC transporter permease, at 584,761 to 585,498
_RS19760
EX31_RS19765: EX31_RS19765 - heme exporter protein CcmB, at 585,716 to 586,375
_RS19765
EX31_RS19770: EX31_RS19770 - cytochrome c biogenesis heme-transporting ATPase CcmA, at 586,397 to 587,038
_RS19770
Position (kb) 584
585
586 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 583.769 kb on + strand, within EX31_RS19745 at 583.772 kb on + strand, within EX31_RS19745 at 583.772 kb on + strand, within EX31_RS19745 at 583.773 kb on - strand, within EX31_RS19745 at 583.798 kb on - strand, within EX31_RS19745 at 583.800 kb on + strand, within EX31_RS19745 at 583.801 kb on - strand, within EX31_RS19745 at 583.809 kb on - strand, within EX31_RS19745 at 583.887 kb on - strand at 583.953 kb on + strand at 583.954 kb on - strand at 583.975 kb on + strand at 584.034 kb on - strand at 584.048 kb on - strand at 584.224 kb on + strand, within EX31_RS19750 at 584.225 kb on - strand, within EX31_RS19750 at 584.225 kb on - strand, within EX31_RS19750 at 584.225 kb on - strand, within EX31_RS19750 at 584.261 kb on + strand, within EX31_RS19750 at 584.369 kb on + strand, within EX31_RS19750 at 584.369 kb on + strand, within EX31_RS19750 at 584.369 kb on + strand, within EX31_RS19750 at 584.369 kb on + strand, within EX31_RS19750 at 584.370 kb on - strand, within EX31_RS19750 at 584.394 kb on - strand, within EX31_RS19750 at 584.394 kb on - strand, within EX31_RS19750 at 584.394 kb on - strand, within EX31_RS19750 at 584.395 kb on + strand, within EX31_RS19750 at 584.395 kb on + strand, within EX31_RS19750 at 584.396 kb on - strand, within EX31_RS19750 at 584.396 kb on - strand, within EX31_RS19750 at 584.396 kb on - strand, within EX31_RS19750 at 584.396 kb on - strand, within EX31_RS19750 at 584.425 kb on + strand, within EX31_RS19750 at 584.425 kb on + strand, within EX31_RS19750 at 584.425 kb on + strand, within EX31_RS19750 at 584.425 kb on + strand, within EX31_RS19750 at 584.426 kb on - strand, within EX31_RS19750 at 584.426 kb on - strand, within EX31_RS19750 at 584.426 kb on - strand, within EX31_RS19750 at 584.426 kb on - strand, within EX31_RS19750 at 584.426 kb on - strand, within EX31_RS19750 at 584.492 kb on + strand at 584.510 kb on + strand at 584.511 kb on - strand at 584.651 kb on - strand, within EX31_RS19755 at 584.706 kb on + strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.707 kb on - strand, within EX31_RS19755 at 584.779 kb on - strand at 584.857 kb on - strand, within EX31_RS19760 at 584.859 kb on + strand, within EX31_RS19760 at 584.860 kb on - strand, within EX31_RS19760 at 584.860 kb on - strand, within EX31_RS19760 at 584.932 kb on - strand, within EX31_RS19760 at 584.975 kb on + strand, within EX31_RS19760 at 584.986 kb on - strand, within EX31_RS19760 at 584.986 kb on - strand, within EX31_RS19760 at 584.992 kb on - strand, within EX31_RS19760 at 584.992 kb on - strand, within EX31_RS19760 at 584.992 kb on - strand, within EX31_RS19760 at 585.063 kb on - strand, within EX31_RS19760 at 585.063 kb on - strand, within EX31_RS19760 at 585.071 kb on - strand, within EX31_RS19760 at 585.083 kb on + strand, within EX31_RS19760 at 585.095 kb on - strand, within EX31_RS19760 at 585.324 kb on + strand, within EX31_RS19760 at 585.324 kb on + strand, within EX31_RS19760 at 585.324 kb on + strand, within EX31_RS19760 at 585.324 kb on + strand, within EX31_RS19760 at 585.324 kb on + strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.325 kb on - strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.326 kb on + strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.327 kb on - strand, within EX31_RS19760 at 585.330 kb on + strand, within EX31_RS19760 at 585.330 kb on + strand, within EX31_RS19760 at 585.330 kb on + strand, within EX31_RS19760 at 585.331 kb on - strand, within EX31_RS19760 at 585.331 kb on - strand, within EX31_RS19760 at 585.331 kb on - strand, within EX31_RS19760 at 585.331 kb on - strand, within EX31_RS19760 at 585.331 kb on - strand, within EX31_RS19760 at 585.345 kb on + strand, within EX31_RS19760 at 585.345 kb on + strand, within EX31_RS19760 at 585.346 kb on - strand, within EX31_RS19760 at 585.346 kb on - strand, within EX31_RS19760 at 585.456 kb on - strand at 585.485 kb on - strand at 585.485 kb on - strand at 585.485 kb on - strand at 585.517 kb on + strand at 585.535 kb on + strand at 585.535 kb on + strand at 585.536 kb on - strand at 585.536 kb on - strand at 585.575 kb on + strand at 585.576 kb on - strand at 585.576 kb on - strand at 585.590 kb on + strand at 585.590 kb on + strand at 585.590 kb on + strand at 585.591 kb on - strand at 585.591 kb on - strand at 585.594 kb on - strand at 585.605 kb on + strand at 585.605 kb on + strand at 585.605 kb on + strand at 585.606 kb on - strand at 585.606 kb on - strand at 585.606 kb on - strand at 585.606 kb on - strand at 585.606 kb on - strand at 585.616 kb on - strand at 585.617 kb on + strand at 585.617 kb on + strand at 585.618 kb on - strand at 585.618 kb on - strand at 585.618 kb on - strand at 585.634 kb on - strand at 585.660 kb on + strand at 585.666 kb on - strand at 585.701 kb on + strand at 585.702 kb on - strand at 585.871 kb on + strand, within EX31_RS19765 at 585.873 kb on + strand, within EX31_RS19765 at 585.873 kb on + strand, within EX31_RS19765 at 585.874 kb on - strand, within EX31_RS19765 at 585.874 kb on - strand, within EX31_RS19765 at 585.874 kb on - strand, within EX31_RS19765 at 585.874 kb on - strand, within EX31_RS19765 at 585.978 kb on - strand, within EX31_RS19765 at 586.088 kb on + strand, within EX31_RS19765 at 586.088 kb on + strand, within EX31_RS19765 at 586.089 kb on - strand, within EX31_RS19765 at 586.089 kb on - strand, within EX31_RS19765 at 586.089 kb on - strand, within EX31_RS19765 at 586.089 kb on - strand, within EX31_RS19765 at 586.158 kb on + strand, within EX31_RS19765 at 586.159 kb on - strand, within EX31_RS19765 at 586.159 kb on - strand, within EX31_RS19765 at 586.199 kb on + strand, within EX31_RS19765 at 586.200 kb on - strand, within EX31_RS19765 at 586.200 kb on - strand, within EX31_RS19765 at 586.316 kb on - strand at 586.382 kb on + strand at 586.382 kb on + strand at 586.382 kb on + strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand at 586.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 583,769 + EX31_RS19745 0.87 -0.5 583,772 + EX31_RS19745 0.88 +2.1 583,772 + EX31_RS19745 0.88 -1.8 583,773 - EX31_RS19745 0.88 +1.5 583,798 - EX31_RS19745 0.89 -0.1 583,800 + EX31_RS19745 0.89 -0.7 583,801 - EX31_RS19745 0.89 -0.3 583,809 - EX31_RS19745 0.89 -0.9 583,887 - +0.8 583,953 + -1.4 583,954 - +0.9 583,975 + -1.1 584,034 - -0.7 584,048 - -0.2 584,224 + EX31_RS19750 0.43 +0.6 584,225 - EX31_RS19750 0.43 -0.4 584,225 - EX31_RS19750 0.43 +0.1 584,225 - EX31_RS19750 0.43 +1.7 584,261 + EX31_RS19750 0.50 +1.0 584,369 + EX31_RS19750 0.72 +0.1 584,369 + EX31_RS19750 0.72 -0.0 584,369 + EX31_RS19750 0.72 +1.0 584,369 + EX31_RS19750 0.72 -0.8 584,370 - EX31_RS19750 0.72 +1.2 584,394 - EX31_RS19750 0.77 -0.2 584,394 - EX31_RS19750 0.77 +0.6 584,394 - EX31_RS19750 0.77 +0.5 584,395 + EX31_RS19750 0.77 +0.3 584,395 + EX31_RS19750 0.77 -0.3 584,396 - EX31_RS19750 0.77 -0.3 584,396 - EX31_RS19750 0.77 +1.0 584,396 - EX31_RS19750 0.77 +0.1 584,396 - EX31_RS19750 0.77 -0.1 584,425 + EX31_RS19750 0.83 +0.0 584,425 + EX31_RS19750 0.83 -0.6 584,425 + EX31_RS19750 0.83 -0.9 584,425 + EX31_RS19750 0.83 -0.9 584,426 - EX31_RS19750 0.83 -0.6 584,426 - EX31_RS19750 0.83 -0.7 584,426 - EX31_RS19750 0.83 +0.2 584,426 - EX31_RS19750 0.83 -1.8 584,426 - EX31_RS19750 0.83 -2.5 584,492 + -0.2 584,510 + -2.3 584,511 - -0.7 584,651 - EX31_RS19755 0.56 +0.7 584,706 + EX31_RS19755 0.77 +0.3 584,707 - EX31_RS19755 0.78 +0.9 584,707 - EX31_RS19755 0.78 +0.8 584,707 - EX31_RS19755 0.78 -1.0 584,707 - EX31_RS19755 0.78 -1.3 584,707 - EX31_RS19755 0.78 -0.6 584,707 - EX31_RS19755 0.78 -1.2 584,707 - EX31_RS19755 0.78 -0.5 584,707 - EX31_RS19755 0.78 +0.3 584,779 - -0.8 584,857 - EX31_RS19760 0.13 -0.0 584,859 + EX31_RS19760 0.13 -1.5 584,860 - EX31_RS19760 0.13 -0.1 584,860 - EX31_RS19760 0.13 -0.6 584,932 - EX31_RS19760 0.23 -1.1 584,975 + EX31_RS19760 0.29 +1.1 584,986 - EX31_RS19760 0.30 -3.1 584,986 - EX31_RS19760 0.30 +0.3 584,992 - EX31_RS19760 0.31 +0.2 584,992 - EX31_RS19760 0.31 +0.7 584,992 - EX31_RS19760 0.31 +1.1 585,063 - EX31_RS19760 0.41 +0.1 585,063 - EX31_RS19760 0.41 -0.7 585,071 - EX31_RS19760 0.42 -0.8 585,083 + EX31_RS19760 0.44 -3.0 585,095 - EX31_RS19760 0.45 -0.5 585,324 + EX31_RS19760 0.76 +0.6 585,324 + EX31_RS19760 0.76 -0.4 585,324 + EX31_RS19760 0.76 -1.1 585,324 + EX31_RS19760 0.76 -0.0 585,324 + EX31_RS19760 0.76 -0.2 585,325 - EX31_RS19760 0.76 -0.2 585,325 - EX31_RS19760 0.76 +0.1 585,325 - EX31_RS19760 0.76 +0.2 585,325 - EX31_RS19760 0.76 +0.0 585,325 - EX31_RS19760 0.76 +1.2 585,325 - EX31_RS19760 0.76 -0.0 585,325 - EX31_RS19760 0.76 +0.0 585,325 - EX31_RS19760 0.76 +0.4 585,325 - EX31_RS19760 0.76 +0.1 585,325 - EX31_RS19760 0.76 -0.4 585,326 + EX31_RS19760 0.77 -0.1 585,326 + EX31_RS19760 0.77 +1.0 585,326 + EX31_RS19760 0.77 +0.5 585,326 + EX31_RS19760 0.77 -0.2 585,326 + EX31_RS19760 0.77 +0.7 585,326 + EX31_RS19760 0.77 +0.0 585,326 + EX31_RS19760 0.77 +0.7 585,326 + EX31_RS19760 0.77 +1.7 585,326 + EX31_RS19760 0.77 +0.1 585,326 + EX31_RS19760 0.77 +1.9 585,326 + EX31_RS19760 0.77 -2.1 585,326 + EX31_RS19760 0.77 -1.8 585,326 + EX31_RS19760 0.77 +0.9 585,326 + EX31_RS19760 0.77 -1.5 585,326 + EX31_RS19760 0.77 -1.4 585,326 + EX31_RS19760 0.77 -0.1 585,327 - EX31_RS19760 0.77 +0.0 585,327 - EX31_RS19760 0.77 -1.3 585,327 - EX31_RS19760 0.77 -0.5 585,327 - EX31_RS19760 0.77 -1.2 585,327 - EX31_RS19760 0.77 +0.3 585,327 - EX31_RS19760 0.77 -0.1 585,327 - EX31_RS19760 0.77 -0.1 585,327 - EX31_RS19760 0.77 +0.3 585,327 - EX31_RS19760 0.77 +0.5 585,327 - EX31_RS19760 0.77 -0.6 585,327 - EX31_RS19760 0.77 -0.3 585,327 - EX31_RS19760 0.77 -0.1 585,327 - EX31_RS19760 0.77 -0.3 585,327 - EX31_RS19760 0.77 -1.3 585,327 - EX31_RS19760 0.77 -1.6 585,327 - EX31_RS19760 0.77 +0.4 585,327 - EX31_RS19760 0.77 -0.9 585,327 - EX31_RS19760 0.77 -0.7 585,327 - EX31_RS19760 0.77 +0.1 585,330 + EX31_RS19760 0.77 -1.2 585,330 + EX31_RS19760 0.77 +0.3 585,330 + EX31_RS19760 0.77 +0.8 585,331 - EX31_RS19760 0.77 -0.9 585,331 - EX31_RS19760 0.77 -0.0 585,331 - EX31_RS19760 0.77 -0.3 585,331 - EX31_RS19760 0.77 -0.8 585,331 - EX31_RS19760 0.77 -0.5 585,345 + EX31_RS19760 0.79 +0.4 585,345 + EX31_RS19760 0.79 -0.7 585,346 - EX31_RS19760 0.79 -0.2 585,346 - EX31_RS19760 0.79 -0.5 585,456 - +1.2 585,485 - -0.8 585,485 - +0.1 585,485 - -0.2 585,517 + +0.3 585,535 + -0.5 585,535 + +0.5 585,536 - -0.3 585,536 - -0.3 585,575 + +1.0 585,576 - +0.1 585,576 - +0.2 585,590 + +0.6 585,590 + -0.7 585,590 + +0.4 585,591 - -0.0 585,591 - +0.1 585,594 - -0.8 585,605 + +1.5 585,605 + +1.1 585,605 + -0.3 585,606 - -0.2 585,606 - -1.3 585,606 - +0.1 585,606 - +0.2 585,606 - -0.7 585,616 - +0.5 585,617 + -0.2 585,617 + -0.7 585,618 - +0.1 585,618 - -0.2 585,618 - -0.8 585,634 - -0.4 585,660 + +0.4 585,666 - -0.5 585,701 + +0.1 585,702 - +1.6 585,871 + EX31_RS19765 0.23 -0.5 585,873 + EX31_RS19765 0.24 +0.6 585,873 + EX31_RS19765 0.24 +0.5 585,874 - EX31_RS19765 0.24 -0.6 585,874 - EX31_RS19765 0.24 -0.4 585,874 - EX31_RS19765 0.24 -1.4 585,874 - EX31_RS19765 0.24 -0.4 585,978 - EX31_RS19765 0.40 -0.5 586,088 + EX31_RS19765 0.56 -0.1 586,088 + EX31_RS19765 0.56 -1.1 586,089 - EX31_RS19765 0.57 -0.0 586,089 - EX31_RS19765 0.57 -0.1 586,089 - EX31_RS19765 0.57 -0.2 586,089 - EX31_RS19765 0.57 -0.4 586,158 + EX31_RS19765 0.67 +0.3 586,159 - EX31_RS19765 0.67 -0.4 586,159 - EX31_RS19765 0.67 +0.2 586,199 + EX31_RS19765 0.73 +0.7 586,200 - EX31_RS19765 0.73 -0.4 586,200 - EX31_RS19765 0.73 +0.0 586,316 - +0.7 586,382 + -0.7 586,382 + -1.3 586,382 + -0.2 586,383 - -0.4 586,383 - -0.1 586,383 - +0.3 586,383 - -0.9 586,383 - -0.7 586,383 - -1.6 586,383 - +0.5 586,383 - +0.8 586,383 - +0.5 586,383 - +1.7 586,383 - -0.4 586,383 - -0.1
Or see this region's nucleotide sequence