Experiment: NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS18715 and EX31_RS18720 are separated by 11 nucleotides EX31_RS18720 and EX31_RS18725 are separated by 63 nucleotides EX31_RS18725 and EX31_RS18730 are separated by 8 nucleotides
EX31_RS18715: EX31_RS18715 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 323,876 to 325,000
_RS18715
EX31_RS18720: EX31_RS18720 - S-formylglutathione hydrolase, at 325,012 to 325,869
_RS18720
EX31_RS18725: EX31_RS18725 - glutathione ABC transporter permease GsiD, at 325,933 to 326,853
_RS18725
EX31_RS18730: EX31_RS18730 - glutathione ABC transporter permease GsiC, at 326,862 to 327,782
_RS18730
Position (kb) 325
326
327 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 324.970 kb on + strand at 324.970 kb on + strand at 324.971 kb on - strand at 324.991 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.994 kb on + strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.995 kb on - strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.998 kb on + strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 324.999 kb on - strand at 325.031 kb on + strand at 325.031 kb on + strand at 325.031 kb on + strand at 325.032 kb on - strand at 325.080 kb on + strand at 325.081 kb on - strand at 325.081 kb on - strand at 325.120 kb on + strand, within EX31_RS18720 at 325.120 kb on + strand, within EX31_RS18720 at 325.121 kb on - strand, within EX31_RS18720 at 325.121 kb on - strand, within EX31_RS18720 at 325.162 kb on + strand, within EX31_RS18720 at 325.185 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.375 kb on + strand, within EX31_RS18720 at 325.376 kb on - strand, within EX31_RS18720 at 325.376 kb on - strand, within EX31_RS18720 at 325.381 kb on + strand, within EX31_RS18720 at 325.381 kb on + strand, within EX31_RS18720 at 325.566 kb on + strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.567 kb on - strand, within EX31_RS18720 at 325.583 kb on - strand, within EX31_RS18720 at 325.611 kb on - strand, within EX31_RS18720 at 325.615 kb on + strand, within EX31_RS18720 at 325.615 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.715 kb on + strand, within EX31_RS18720 at 325.716 kb on - strand, within EX31_RS18720 at 325.787 kb on - strand at 325.798 kb on + strand at 325.799 kb on - strand at 325.803 kb on + strand at 325.803 kb on + strand at 325.804 kb on - strand at 325.881 kb on + strand at 325.881 kb on + strand at 325.881 kb on + strand at 325.887 kb on + strand at 325.887 kb on + strand at 325.997 kb on - strand at 326.027 kb on - strand, within EX31_RS18725 at 326.053 kb on - strand, within EX31_RS18725 at 326.172 kb on - strand, within EX31_RS18725 at 326.172 kb on - strand, within EX31_RS18725 at 326.260 kb on - strand, within EX31_RS18725 at 326.260 kb on - strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.261 kb on + strand, within EX31_RS18725 at 326.262 kb on - strand, within EX31_RS18725 at 326.262 kb on - strand, within EX31_RS18725 at 326.262 kb on - strand, within EX31_RS18725 at 326.459 kb on + strand, within EX31_RS18725 at 326.460 kb on - strand, within EX31_RS18725 at 326.460 kb on - strand, within EX31_RS18725 at 326.460 kb on - strand, within EX31_RS18725 at 326.460 kb on - strand, within EX31_RS18725 at 326.460 kb on - strand, within EX31_RS18725 at 326.568 kb on + strand, within EX31_RS18725 at 326.569 kb on - strand, within EX31_RS18725 at 326.634 kb on - strand, within EX31_RS18725 at 326.859 kb on + strand at 326.860 kb on - strand at 326.870 kb on - strand at 327.083 kb on + strand, within EX31_RS18730 at 327.083 kb on + strand, within EX31_RS18730 at 327.084 kb on - strand, within EX31_RS18730 at 327.084 kb on - strand, within EX31_RS18730 at 327.164 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.170 kb on + strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.171 kb on - strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.172 kb on + strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.173 kb on - strand, within EX31_RS18730 at 327.263 kb on + strand, within EX31_RS18730 at 327.263 kb on + strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.264 kb on - strand, within EX31_RS18730 at 327.265 kb on + strand, within EX31_RS18730 at 327.265 kb on + strand, within EX31_RS18730 at 327.265 kb on + strand, within EX31_RS18730 at 327.265 kb on + strand, within EX31_RS18730 at 327.265 kb on + strand, within EX31_RS18730 at 327.266 kb on - strand, within EX31_RS18730 at 327.339 kb on - strand, within EX31_RS18730 at 327.339 kb on - strand, within EX31_RS18730 at 327.438 kb on + strand, within EX31_RS18730 at 327.439 kb on - strand, within EX31_RS18730 at 327.439 kb on - strand, within EX31_RS18730 at 327.609 kb on - strand, within EX31_RS18730 at 327.609 kb on - strand, within EX31_RS18730 at 327.709 kb on - strand at 327.756 kb on + strand at 327.757 kb on - strand at 327.772 kb on + strand at 327.776 kb on + strand at 327.776 kb on + strand at 327.777 kb on - strand at 327.777 kb on - strand at 327.777 kb on - strand at 327.777 kb on - strand at 327.777 kb on - strand at 327.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NL-CCM; Mixed culture; Sphingobium sp. HT1-2 2:1 starting OD to Azospirillum remove 324,970 + +0.0 324,970 + +1.2 324,971 - +0.5 324,991 + +0.4 324,994 + -0.2 324,994 + -0.4 324,994 + -1.0 324,994 + -1.9 324,994 + -0.1 324,995 - -0.2 324,995 - +0.3 324,995 - +0.2 324,995 - +1.3 324,995 - +2.2 324,995 - +1.0 324,995 - -0.5 324,995 - -0.8 324,998 + -1.2 324,998 + -0.6 324,998 + +0.1 324,998 + +0.4 324,999 - -1.0 324,999 - -1.5 324,999 - -1.5 324,999 - -0.3 324,999 - -0.1 325,031 + -1.0 325,031 + +0.5 325,031 + +0.2 325,032 - -0.4 325,080 + -0.2 325,081 - -1.1 325,081 - -2.2 325,120 + EX31_RS18720 0.13 -0.7 325,120 + EX31_RS18720 0.13 -0.5 325,121 - EX31_RS18720 0.13 -0.9 325,121 - EX31_RS18720 0.13 +0.2 325,162 + EX31_RS18720 0.17 +1.0 325,185 + EX31_RS18720 0.20 +0.1 325,375 + EX31_RS18720 0.42 +0.1 325,375 + EX31_RS18720 0.42 -0.8 325,375 + EX31_RS18720 0.42 -0.7 325,375 + EX31_RS18720 0.42 -0.7 325,376 - EX31_RS18720 0.42 -1.0 325,376 - EX31_RS18720 0.42 +0.3 325,381 + EX31_RS18720 0.43 -0.8 325,381 + EX31_RS18720 0.43 -1.6 325,566 + EX31_RS18720 0.65 +0.1 325,567 - EX31_RS18720 0.65 +0.2 325,567 - EX31_RS18720 0.65 -0.2 325,567 - EX31_RS18720 0.65 -0.1 325,567 - EX31_RS18720 0.65 +2.0 325,583 - EX31_RS18720 0.67 -2.0 325,611 - EX31_RS18720 0.70 +0.4 325,615 + EX31_RS18720 0.70 -0.4 325,615 + EX31_RS18720 0.70 -1.2 325,715 + EX31_RS18720 0.82 -0.0 325,715 + EX31_RS18720 0.82 +0.3 325,715 + EX31_RS18720 0.82 +0.4 325,716 - EX31_RS18720 0.82 +0.4 325,787 - +1.1 325,798 + +0.3 325,799 - +0.1 325,803 + +0.2 325,803 + -0.9 325,804 - -0.3 325,881 + -0.7 325,881 + -0.2 325,881 + -0.5 325,887 + +0.2 325,887 + -0.5 325,997 - +0.6 326,027 - EX31_RS18725 0.10 -1.2 326,053 - EX31_RS18725 0.13 +0.5 326,172 - EX31_RS18725 0.26 +0.2 326,172 - EX31_RS18725 0.26 -0.5 326,260 - EX31_RS18725 0.36 -0.3 326,260 - EX31_RS18725 0.36 +0.5 326,261 + EX31_RS18725 0.36 +0.0 326,261 + EX31_RS18725 0.36 -1.1 326,261 + EX31_RS18725 0.36 -1.0 326,261 + EX31_RS18725 0.36 -1.0 326,261 + EX31_RS18725 0.36 -2.3 326,261 + EX31_RS18725 0.36 -0.9 326,261 + EX31_RS18725 0.36 +0.1 326,261 + EX31_RS18725 0.36 -1.3 326,261 + EX31_RS18725 0.36 -0.2 326,262 - EX31_RS18725 0.36 -0.0 326,262 - EX31_RS18725 0.36 -0.6 326,262 - EX31_RS18725 0.36 -0.0 326,459 + EX31_RS18725 0.57 -0.9 326,460 - EX31_RS18725 0.57 -0.8 326,460 - EX31_RS18725 0.57 -1.5 326,460 - EX31_RS18725 0.57 -2.8 326,460 - EX31_RS18725 0.57 -0.9 326,460 - EX31_RS18725 0.57 -0.4 326,568 + EX31_RS18725 0.69 -0.3 326,569 - EX31_RS18725 0.69 +0.5 326,634 - EX31_RS18725 0.76 +0.5 326,859 + -0.7 326,860 - +0.0 326,870 - +0.8 327,083 + EX31_RS18730 0.24 -0.1 327,083 + EX31_RS18730 0.24 -0.4 327,084 - EX31_RS18730 0.24 +0.4 327,084 - EX31_RS18730 0.24 -0.5 327,164 + EX31_RS18730 0.33 +0.5 327,170 + EX31_RS18730 0.33 -0.7 327,170 + EX31_RS18730 0.33 +0.8 327,170 + EX31_RS18730 0.33 +0.2 327,170 + EX31_RS18730 0.33 +0.5 327,170 + EX31_RS18730 0.33 +0.2 327,170 + EX31_RS18730 0.33 -0.4 327,171 - EX31_RS18730 0.34 +0.1 327,171 - EX31_RS18730 0.34 +0.2 327,171 - EX31_RS18730 0.34 -1.9 327,171 - EX31_RS18730 0.34 +0.6 327,171 - EX31_RS18730 0.34 +0.4 327,171 - EX31_RS18730 0.34 -0.6 327,171 - EX31_RS18730 0.34 +0.0 327,171 - EX31_RS18730 0.34 -1.6 327,172 + EX31_RS18730 0.34 -1.0 327,172 + EX31_RS18730 0.34 -1.1 327,172 + EX31_RS18730 0.34 -2.3 327,172 + EX31_RS18730 0.34 +0.7 327,172 + EX31_RS18730 0.34 -1.0 327,172 + EX31_RS18730 0.34 -0.5 327,173 - EX31_RS18730 0.34 -1.2 327,173 - EX31_RS18730 0.34 -0.2 327,173 - EX31_RS18730 0.34 +0.2 327,173 - EX31_RS18730 0.34 +0.8 327,173 - EX31_RS18730 0.34 -0.5 327,173 - EX31_RS18730 0.34 -0.0 327,263 + EX31_RS18730 0.44 -0.6 327,263 + EX31_RS18730 0.44 -0.4 327,264 - EX31_RS18730 0.44 +0.1 327,264 - EX31_RS18730 0.44 -0.2 327,264 - EX31_RS18730 0.44 +0.1 327,264 - EX31_RS18730 0.44 -0.5 327,264 - EX31_RS18730 0.44 +0.8 327,264 - EX31_RS18730 0.44 -0.5 327,265 + EX31_RS18730 0.44 -0.5 327,265 + EX31_RS18730 0.44 +0.0 327,265 + EX31_RS18730 0.44 +0.5 327,265 + EX31_RS18730 0.44 -0.3 327,265 + EX31_RS18730 0.44 +0.2 327,266 - EX31_RS18730 0.44 -1.4 327,339 - EX31_RS18730 0.52 -0.3 327,339 - EX31_RS18730 0.52 +0.9 327,438 + EX31_RS18730 0.63 -1.8 327,439 - EX31_RS18730 0.63 +0.1 327,439 - EX31_RS18730 0.63 -2.7 327,609 - EX31_RS18730 0.81 -0.3 327,609 - EX31_RS18730 0.81 -1.2 327,709 - -0.8 327,756 + +0.5 327,757 - -1.5 327,772 + -0.1 327,776 + -0.3 327,776 + -1.7 327,777 - -0.8 327,777 - -0.9 327,777 - +1.2 327,777 - +0.4 327,777 - -1.3 327,808 - -1.5
Or see this region's nucleotide sequence