Experiment: NF_with_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10195 and EX31_RS10200 are separated by 150 nucleotides EX31_RS10200 and EX31_RS10205 overlap by 4 nucleotides EX31_RS10205 and EX31_RS10210 are separated by 9 nucleotides
EX31_RS10195: EX31_RS10195 - LysR family transcriptional regulator, at 252,000 to 253,229
_RS10195
EX31_RS10200: EX31_RS10200 - 4-oxalmesaconate hydratase, at 253,380 to 254,126
_RS10200
EX31_RS10205: EX31_RS10205 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 254,123 to 254,848
_RS10205
EX31_RS10210: EX31_RS10210 - 4-oxalomesaconate tautomerase, at 254,858 to 255,982
_RS10210
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 252.449 kb on - strand, within EX31_RS10195 at 252.449 kb on - strand, within EX31_RS10195 at 252.476 kb on + strand, within EX31_RS10195 at 252.689 kb on + strand, within EX31_RS10195 at 252.792 kb on + strand, within EX31_RS10195 at 252.793 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.855 kb on - strand, within EX31_RS10195 at 252.879 kb on - strand, within EX31_RS10195 at 252.902 kb on + strand, within EX31_RS10195 at 252.903 kb on - strand, within EX31_RS10195 at 252.911 kb on - strand, within EX31_RS10195 at 252.911 kb on - strand, within EX31_RS10195 at 252.955 kb on - strand, within EX31_RS10195 at 253.041 kb on + strand, within EX31_RS10195 at 253.406 kb on + strand at 253.406 kb on + strand at 253.407 kb on - strand at 253.417 kb on + strand at 253.479 kb on - strand, within EX31_RS10200 at 253.479 kb on - strand, within EX31_RS10200 at 253.479 kb on - strand, within EX31_RS10200 at 253.562 kb on + strand, within EX31_RS10200 at 253.563 kb on - strand, within EX31_RS10200 at 253.717 kb on + strand, within EX31_RS10200 at 253.758 kb on + strand, within EX31_RS10200 at 253.764 kb on + strand, within EX31_RS10200 at 253.764 kb on + strand, within EX31_RS10200 at 253.765 kb on - strand, within EX31_RS10200 at 253.765 kb on - strand, within EX31_RS10200 at 253.809 kb on - strand, within EX31_RS10200 at 253.827 kb on + strand, within EX31_RS10200 at 253.827 kb on + strand, within EX31_RS10200 at 253.828 kb on - strand, within EX31_RS10200 at 253.828 kb on - strand, within EX31_RS10200 at 253.829 kb on + strand, within EX31_RS10200 at 253.829 kb on + strand, within EX31_RS10200 at 253.830 kb on - strand, within EX31_RS10200 at 253.995 kb on + strand, within EX31_RS10200 at 253.996 kb on - strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.998 kb on + strand, within EX31_RS10200 at 253.999 kb on - strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.039 kb on + strand, within EX31_RS10200 at 254.119 kb on + strand at 254.120 kb on - strand at 254.120 kb on - strand at 254.161 kb on + strand at 254.177 kb on - strand at 254.220 kb on + strand, within EX31_RS10205 at 254.221 kb on - strand, within EX31_RS10205 at 254.271 kb on + strand, within EX31_RS10205 at 254.551 kb on + strand, within EX31_RS10205 at 254.551 kb on + strand, within EX31_RS10205 at 254.552 kb on - strand, within EX31_RS10205 at 254.607 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.765 kb on + strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.766 kb on - strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 254.770 kb on + strand, within EX31_RS10205 at 255.103 kb on + strand, within EX31_RS10210 at 255.104 kb on - strand, within EX31_RS10210
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with_fullN remove 252,449 - EX31_RS10195 0.37 -0.4 252,449 - EX31_RS10195 0.37 -1.5 252,476 + EX31_RS10195 0.39 +0.5 252,689 + EX31_RS10195 0.56 -2.1 252,792 + EX31_RS10195 0.64 +0.5 252,793 - EX31_RS10195 0.64 +1.2 252,855 - EX31_RS10195 0.70 -0.2 252,855 - EX31_RS10195 0.70 -0.3 252,855 - EX31_RS10195 0.70 +0.3 252,855 - EX31_RS10195 0.70 -1.4 252,879 - EX31_RS10195 0.71 +0.4 252,902 + EX31_RS10195 0.73 -2.7 252,903 - EX31_RS10195 0.73 -0.1 252,911 - EX31_RS10195 0.74 -1.0 252,911 - EX31_RS10195 0.74 -1.3 252,955 - EX31_RS10195 0.78 -0.2 253,041 + EX31_RS10195 0.85 +1.3 253,406 + +0.1 253,406 + -0.2 253,407 - +0.1 253,417 + +0.8 253,479 - EX31_RS10200 0.13 +1.3 253,479 - EX31_RS10200 0.13 -0.2 253,479 - EX31_RS10200 0.13 -0.5 253,562 + EX31_RS10200 0.24 +0.9 253,563 - EX31_RS10200 0.24 -0.2 253,717 + EX31_RS10200 0.45 -1.4 253,758 + EX31_RS10200 0.51 +0.3 253,764 + EX31_RS10200 0.51 -0.2 253,764 + EX31_RS10200 0.51 +0.5 253,765 - EX31_RS10200 0.52 -0.1 253,765 - EX31_RS10200 0.52 +1.7 253,809 - EX31_RS10200 0.57 +2.0 253,827 + EX31_RS10200 0.60 -0.4 253,827 + EX31_RS10200 0.60 +2.0 253,828 - EX31_RS10200 0.60 -1.6 253,828 - EX31_RS10200 0.60 +1.2 253,829 + EX31_RS10200 0.60 +3.2 253,829 + EX31_RS10200 0.60 +0.6 253,830 - EX31_RS10200 0.60 -0.0 253,995 + EX31_RS10200 0.82 -0.8 253,996 - EX31_RS10200 0.82 -1.2 253,998 + EX31_RS10200 0.83 +0.0 253,998 + EX31_RS10200 0.83 -0.3 253,999 - EX31_RS10200 0.83 -0.6 254,039 + EX31_RS10200 0.88 +0.3 254,039 + EX31_RS10200 0.88 +0.5 254,119 + -0.3 254,120 - +1.3 254,120 - +0.8 254,161 + +0.3 254,177 - +0.3 254,220 + EX31_RS10205 0.13 -0.2 254,221 - EX31_RS10205 0.13 -0.0 254,271 + EX31_RS10205 0.20 -0.4 254,551 + EX31_RS10205 0.59 +0.2 254,551 + EX31_RS10205 0.59 -0.4 254,552 - EX31_RS10205 0.59 -1.5 254,607 + EX31_RS10205 0.67 +0.2 254,765 + EX31_RS10205 0.88 -0.7 254,765 + EX31_RS10205 0.88 +1.3 254,765 + EX31_RS10205 0.88 +0.5 254,765 + EX31_RS10205 0.88 +0.6 254,765 + EX31_RS10205 0.88 -0.2 254,765 + EX31_RS10205 0.88 +1.1 254,766 - EX31_RS10205 0.89 -1.0 254,766 - EX31_RS10205 0.89 -0.3 254,766 - EX31_RS10205 0.89 -0.3 254,770 + EX31_RS10205 0.89 -0.3 254,770 + EX31_RS10205 0.89 -0.9 254,770 + EX31_RS10205 0.89 +0.4 255,103 + EX31_RS10210 0.22 -0.4 255,104 - EX31_RS10210 0.22 -2.6
Or see this region's nucleotide sequence