Strain Fitness in Rahnella sp. WP5 around EX31_RS17135

Experiment: NF_with_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS17130 and EX31_RS17135 overlap by 4 nucleotidesEX31_RS17135 and EX31_RS17140 are separated by 52 nucleotides EX31_RS17130: EX31_RS17130 - UDP-N-acetylmuramate dehydrogenase, at 418 to 1,455 _RS17130 EX31_RS17135: EX31_RS17135 - bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA, at 1,452 to 2,411 _RS17135 EX31_RS17140: EX31_RS17140 - type I pantothenate kinase, at 2,464 to 3,414 _RS17140 Position (kb) 1 2 3Strain fitness (log2 ratio) -1 0 1at 1.463 kb on + strandat 2.418 kb on + strandat 2.419 kb on - strandat 2.428 kb on + strandat 2.429 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with_fullN
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1,463 + -0.7
2,418 + +0.4
2,419 - +1.2
2,428 + +1.0
2,429 - +0.9

Or see this region's nucleotide sequence