Experiment: NF_with_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15340 and EX31_RS15345 are separated by 49 nucleotides EX31_RS15345 and EX31_RS15350 overlap by 14 nucleotides EX31_RS15350 and EX31_RS15355 are separated by 154 nucleotides EX31_RS15355 and EX31_RS15360 are separated by 17 nucleotides
EX31_RS15340: EX31_RS15340 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 19,071 to 20,444
_RS15340
EX31_RS15345: EX31_RS15345 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 20,494 to 21,879
_RS15345
EX31_RS15350: EX31_RS15350 - nitrogen fixation protein NifX, at 21,866 to 22,330
_RS15350
EX31_RS15355: EX31_RS15355 - hypothetical protein, at 22,485 to 22,823
_RS15355
EX31_RS15360: EX31_RS15360 - Fe-S cluster assembly protein NifU, at 22,841 to 23,668
_RS15360
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2 at 19.539 kb on + strand, within EX31_RS15340 at 19.540 kb on - strand, within EX31_RS15340 at 19.685 kb on + strand, within EX31_RS15340 at 19.686 kb on - strand, within EX31_RS15340 at 19.748 kb on + strand, within EX31_RS15340 at 19.749 kb on - strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.863 kb on + strand, within EX31_RS15340 at 19.894 kb on - strand, within EX31_RS15340 at 19.957 kb on + strand, within EX31_RS15340 at 20.028 kb on - strand, within EX31_RS15340 at 20.058 kb on + strand, within EX31_RS15340 at 20.058 kb on + strand, within EX31_RS15340 at 20.058 kb on + strand, within EX31_RS15340 at 20.058 kb on + strand, within EX31_RS15340 at 20.058 kb on + strand, within EX31_RS15340 at 20.059 kb on - strand, within EX31_RS15340 at 20.059 kb on - strand, within EX31_RS15340 at 20.099 kb on - strand, within EX31_RS15340 at 20.108 kb on - strand, within EX31_RS15340 at 20.108 kb on - strand, within EX31_RS15340 at 20.166 kb on - strand, within EX31_RS15340 at 20.238 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.283 kb on + strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.284 kb on - strand, within EX31_RS15340 at 20.285 kb on + strand, within EX31_RS15340 at 20.286 kb on - strand, within EX31_RS15340 at 20.286 kb on - strand, within EX31_RS15340 at 20.286 kb on - strand, within EX31_RS15340 at 20.286 kb on - strand, within EX31_RS15340 at 20.293 kb on - strand, within EX31_RS15340 at 20.387 kb on + strand at 20.387 kb on + strand at 20.387 kb on + strand at 20.387 kb on + strand at 20.387 kb on + strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.388 kb on - strand at 20.493 kb on + strand at 20.493 kb on + strand at 20.493 kb on + strand at 20.494 kb on - strand at 20.511 kb on + strand at 20.511 kb on + strand at 20.511 kb on + strand at 20.541 kb on + strand at 20.542 kb on - strand at 20.709 kb on + strand, within EX31_RS15345 at 20.710 kb on - strand, within EX31_RS15345 at 20.710 kb on - strand, within EX31_RS15345 at 20.710 kb on - strand, within EX31_RS15345 at 20.710 kb on - strand, within EX31_RS15345 at 20.737 kb on - strand, within EX31_RS15345 at 20.869 kb on - strand, within EX31_RS15345 at 20.892 kb on + strand, within EX31_RS15345 at 20.994 kb on + strand, within EX31_RS15345 at 20.994 kb on + strand, within EX31_RS15345 at 20.995 kb on - strand, within EX31_RS15345 at 20.995 kb on - strand, within EX31_RS15345 at 21.051 kb on - strand, within EX31_RS15345 at 21.051 kb on - strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.061 kb on + strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.062 kb on - strand, within EX31_RS15345 at 21.104 kb on + strand, within EX31_RS15345 at 21.415 kb on - strand, within EX31_RS15345 at 21.435 kb on + strand, within EX31_RS15345 at 21.436 kb on - strand, within EX31_RS15345 at 21.590 kb on + strand, within EX31_RS15345 at 21.700 kb on + strand, within EX31_RS15345 at 21.700 kb on + strand, within EX31_RS15345 at 21.739 kb on + strand, within EX31_RS15345 at 21.740 kb on - strand, within EX31_RS15345 at 21.812 kb on - strand at 21.992 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.112 kb on + strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.113 kb on - strand, within EX31_RS15350 at 22.345 kb on + strand at 22.346 kb on - strand at 22.364 kb on - strand at 22.405 kb on + strand at 22.405 kb on + strand at 22.406 kb on - strand at 22.419 kb on - strand at 22.461 kb on + strand at 22.461 kb on + strand at 22.462 kb on - strand at 22.462 kb on - strand at 22.493 kb on - strand at 22.573 kb on + strand, within EX31_RS15355 at 22.573 kb on + strand, within EX31_RS15355 at 22.573 kb on + strand, within EX31_RS15355 at 22.574 kb on - strand, within EX31_RS15355 at 22.574 kb on - strand, within EX31_RS15355 at 22.645 kb on + strand, within EX31_RS15355 at 22.645 kb on + strand, within EX31_RS15355 at 22.646 kb on - strand, within EX31_RS15355 at 22.646 kb on - strand, within EX31_RS15355 at 22.696 kb on + strand, within EX31_RS15355 at 22.696 kb on + strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.700 kb on - strand, within EX31_RS15355 at 22.747 kb on + strand, within EX31_RS15355 at 22.748 kb on - strand, within EX31_RS15355 at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.799 kb on + strand at 22.800 kb on - strand at 22.800 kb on - strand at 22.835 kb on - strand at 22.850 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with_fullN remove 19,539 + EX31_RS15340 0.34 -0.8 19,540 - EX31_RS15340 0.34 -0.8 19,685 + EX31_RS15340 0.45 -1.2 19,686 - EX31_RS15340 0.45 -0.4 19,748 + EX31_RS15340 0.49 -0.2 19,749 - EX31_RS15340 0.49 -0.8 19,787 + EX31_RS15340 0.52 -0.1 19,787 + EX31_RS15340 0.52 +0.9 19,787 + EX31_RS15340 0.52 -0.2 19,787 + EX31_RS15340 0.52 +0.8 19,787 + EX31_RS15340 0.52 +1.0 19,787 + EX31_RS15340 0.52 -0.2 19,787 + EX31_RS15340 0.52 +1.1 19,788 - EX31_RS15340 0.52 +0.1 19,788 - EX31_RS15340 0.52 -1.0 19,788 - EX31_RS15340 0.52 -0.6 19,788 - EX31_RS15340 0.52 -0.0 19,863 + EX31_RS15340 0.58 +0.3 19,894 - EX31_RS15340 0.60 +0.4 19,957 + EX31_RS15340 0.64 +0.8 20,028 - EX31_RS15340 0.70 +0.3 20,058 + EX31_RS15340 0.72 +0.4 20,058 + EX31_RS15340 0.72 +0.3 20,058 + EX31_RS15340 0.72 -0.3 20,058 + EX31_RS15340 0.72 +0.4 20,058 + EX31_RS15340 0.72 +0.9 20,059 - EX31_RS15340 0.72 +0.4 20,059 - EX31_RS15340 0.72 -0.3 20,099 - EX31_RS15340 0.75 +1.0 20,108 - EX31_RS15340 0.75 -1.2 20,108 - EX31_RS15340 0.75 -0.9 20,166 - EX31_RS15340 0.80 +0.5 20,238 + EX31_RS15340 0.85 +1.2 20,283 + EX31_RS15340 0.88 -0.4 20,283 + EX31_RS15340 0.88 -1.4 20,283 + EX31_RS15340 0.88 -0.5 20,283 + EX31_RS15340 0.88 +0.0 20,283 + EX31_RS15340 0.88 -0.3 20,283 + EX31_RS15340 0.88 +0.5 20,283 + EX31_RS15340 0.88 +0.1 20,283 + EX31_RS15340 0.88 -0.6 20,283 + EX31_RS15340 0.88 -0.8 20,283 + EX31_RS15340 0.88 +0.9 20,283 + EX31_RS15340 0.88 -0.2 20,283 + EX31_RS15340 0.88 -1.2 20,283 + EX31_RS15340 0.88 +0.7 20,283 + EX31_RS15340 0.88 +1.0 20,283 + EX31_RS15340 0.88 -1.5 20,283 + EX31_RS15340 0.88 -0.5 20,283 + EX31_RS15340 0.88 -0.3 20,283 + EX31_RS15340 0.88 +1.3 20,283 + EX31_RS15340 0.88 -0.1 20,283 + EX31_RS15340 0.88 +0.7 20,283 + EX31_RS15340 0.88 +0.2 20,283 + EX31_RS15340 0.88 -0.3 20,283 + EX31_RS15340 0.88 -1.1 20,283 + EX31_RS15340 0.88 -0.3 20,283 + EX31_RS15340 0.88 -0.0 20,283 + EX31_RS15340 0.88 -0.2 20,283 + EX31_RS15340 0.88 -0.3 20,283 + EX31_RS15340 0.88 +0.2 20,283 + EX31_RS15340 0.88 -0.4 20,283 + EX31_RS15340 0.88 -0.7 20,283 + EX31_RS15340 0.88 -0.2 20,283 + EX31_RS15340 0.88 +0.2 20,283 + EX31_RS15340 0.88 -0.4 20,284 - EX31_RS15340 0.88 -0.5 20,284 - EX31_RS15340 0.88 -1.2 20,284 - EX31_RS15340 0.88 +1.2 20,284 - EX31_RS15340 0.88 -0.2 20,284 - EX31_RS15340 0.88 -1.5 20,284 - EX31_RS15340 0.88 -0.2 20,284 - EX31_RS15340 0.88 -1.2 20,284 - EX31_RS15340 0.88 -0.0 20,284 - EX31_RS15340 0.88 -0.1 20,284 - EX31_RS15340 0.88 +0.5 20,284 - EX31_RS15340 0.88 -0.4 20,284 - EX31_RS15340 0.88 -0.4 20,284 - EX31_RS15340 0.88 +0.9 20,284 - EX31_RS15340 0.88 +0.8 20,285 + EX31_RS15340 0.88 -0.2 20,286 - EX31_RS15340 0.88 -0.9 20,286 - EX31_RS15340 0.88 -1.1 20,286 - EX31_RS15340 0.88 +0.0 20,286 - EX31_RS15340 0.88 -0.9 20,293 - EX31_RS15340 0.89 +0.8 20,387 + -0.1 20,387 + +1.9 20,387 + -0.7 20,387 + -0.3 20,387 + -0.4 20,388 - +0.2 20,388 - -0.4 20,388 - +0.8 20,388 - -0.9 20,388 - -0.3 20,388 - -0.5 20,388 - +1.1 20,493 + -0.6 20,493 + -0.3 20,493 + +0.4 20,494 - +0.8 20,511 + -0.3 20,511 + -0.7 20,511 + +1.5 20,541 + +0.2 20,542 - -0.2 20,709 + EX31_RS15345 0.16 +0.5 20,710 - EX31_RS15345 0.16 -0.3 20,710 - EX31_RS15345 0.16 -0.7 20,710 - EX31_RS15345 0.16 +1.3 20,710 - EX31_RS15345 0.16 +0.7 20,737 - EX31_RS15345 0.18 +0.8 20,869 - EX31_RS15345 0.27 +0.6 20,892 + EX31_RS15345 0.29 +0.8 20,994 + EX31_RS15345 0.36 -0.4 20,994 + EX31_RS15345 0.36 +1.2 20,995 - EX31_RS15345 0.36 +0.4 20,995 - EX31_RS15345 0.36 -1.6 21,051 - EX31_RS15345 0.40 -0.1 21,051 - EX31_RS15345 0.40 -0.6 21,061 + EX31_RS15345 0.41 +1.1 21,061 + EX31_RS15345 0.41 -0.0 21,061 + EX31_RS15345 0.41 -0.6 21,061 + EX31_RS15345 0.41 +0.0 21,061 + EX31_RS15345 0.41 +0.1 21,061 + EX31_RS15345 0.41 +2.0 21,061 + EX31_RS15345 0.41 -0.1 21,061 + EX31_RS15345 0.41 +0.2 21,061 + EX31_RS15345 0.41 +0.3 21,061 + EX31_RS15345 0.41 +0.9 21,061 + EX31_RS15345 0.41 -0.4 21,061 + EX31_RS15345 0.41 +1.2 21,062 - EX31_RS15345 0.41 -0.7 21,062 - EX31_RS15345 0.41 -0.5 21,062 - EX31_RS15345 0.41 +0.3 21,062 - EX31_RS15345 0.41 -0.8 21,062 - EX31_RS15345 0.41 -0.7 21,062 - EX31_RS15345 0.41 -1.1 21,062 - EX31_RS15345 0.41 -0.3 21,062 - EX31_RS15345 0.41 +1.4 21,062 - EX31_RS15345 0.41 +0.1 21,062 - EX31_RS15345 0.41 -0.2 21,062 - EX31_RS15345 0.41 -0.8 21,104 + EX31_RS15345 0.44 -1.1 21,415 - EX31_RS15345 0.66 +0.0 21,435 + EX31_RS15345 0.68 +1.5 21,436 - EX31_RS15345 0.68 -1.0 21,590 + EX31_RS15345 0.79 -0.1 21,700 + EX31_RS15345 0.87 +0.1 21,700 + EX31_RS15345 0.87 -0.4 21,739 + EX31_RS15345 0.90 -0.9 21,740 - EX31_RS15345 0.90 +0.3 21,812 - +0.3 21,992 + EX31_RS15350 0.27 -0.4 22,112 + EX31_RS15350 0.53 +0.7 22,112 + EX31_RS15350 0.53 +0.3 22,112 + EX31_RS15350 0.53 +0.3 22,113 - EX31_RS15350 0.53 -0.8 22,113 - EX31_RS15350 0.53 -1.3 22,113 - EX31_RS15350 0.53 +0.3 22,113 - EX31_RS15350 0.53 -2.2 22,345 + +1.2 22,346 - -0.5 22,364 - -0.1 22,405 + -0.7 22,405 + -0.3 22,406 - +0.2 22,419 - -0.2 22,461 + -0.7 22,461 + +0.0 22,462 - -2.2 22,462 - +0.6 22,493 - -0.2 22,573 + EX31_RS15355 0.26 +1.1 22,573 + EX31_RS15355 0.26 -0.3 22,573 + EX31_RS15355 0.26 +1.5 22,574 - EX31_RS15355 0.26 -0.7 22,574 - EX31_RS15355 0.26 -1.0 22,645 + EX31_RS15355 0.47 +0.1 22,645 + EX31_RS15355 0.47 +0.9 22,646 - EX31_RS15355 0.47 +0.7 22,646 - EX31_RS15355 0.47 -0.0 22,696 + EX31_RS15355 0.62 -0.2 22,696 + EX31_RS15355 0.62 -0.2 22,700 - EX31_RS15355 0.63 -1.2 22,700 - EX31_RS15355 0.63 -0.2 22,700 - EX31_RS15355 0.63 +1.1 22,700 - EX31_RS15355 0.63 -1.2 22,747 + EX31_RS15355 0.77 -0.6 22,748 - EX31_RS15355 0.78 +0.8 22,799 + +0.9 22,799 + -0.1 22,799 + -0.5 22,799 + -0.8 22,799 + +0.5 22,799 + +1.6 22,800 - +1.8 22,800 - +0.6 22,835 - +1.5 22,850 + +0.5
Or see this region's nucleotide sequence