Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS22485 and EX31_RS22490 are separated by 60 nucleotides EX31_RS22490 and EX31_RS22495 are separated by 95 nucleotides EX31_RS22495 and EX31_RS22500 are separated by 114 nucleotides EX31_RS22500 and EX31_RS22505 are separated by 183 nucleotides
EX31_RS22485: EX31_RS22485 - mechanosensitive channel MscK, at 248,376 to 251,771
_RS22485
EX31_RS22490: EX31_RS22490 - pleiotropic regulatory protein RsmS, at 251,832 to 252,005
_RS22490
EX31_RS22495: EX31_RS22495 - prephenate dehydrogenase, at 252,101 to 252,637
_RS22495
EX31_RS22500: EX31_RS22500 - DUF454 family protein, at 252,752 to 253,120
_RS22500
EX31_RS22505: EX31_RS22505 - adenine phosphoribosyltransferase, at 253,304 to 253,855
_RS22505
Position (kb) 252
253 Strain fitness (log2 ratio) -2
-1
0
1
2 at 251.240 kb on + strand, within EX31_RS22485 at 251.241 kb on - strand, within EX31_RS22485 at 251.295 kb on - strand, within EX31_RS22485 at 251.295 kb on - strand, within EX31_RS22485 at 251.430 kb on + strand, within EX31_RS22485 at 251.430 kb on + strand, within EX31_RS22485 at 251.431 kb on - strand, within EX31_RS22485 at 251.431 kb on - strand, within EX31_RS22485 at 251.431 kb on - strand, within EX31_RS22485 at 251.431 kb on - strand, within EX31_RS22485 at 251.464 kb on + strand at 251.464 kb on + strand at 251.465 kb on - strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.568 kb on + strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.569 kb on - strand at 251.595 kb on + strand at 251.595 kb on + strand at 251.595 kb on + strand at 251.595 kb on + strand at 251.597 kb on + strand at 251.597 kb on + strand at 251.598 kb on - strand at 251.598 kb on - strand at 251.598 kb on - strand at 251.598 kb on - strand at 251.627 kb on + strand at 251.627 kb on + strand at 251.628 kb on - strand at 251.651 kb on + strand at 251.651 kb on + strand at 251.651 kb on + strand at 251.651 kb on + strand at 251.651 kb on + strand at 251.651 kb on + strand at 251.652 kb on - strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.679 kb on + strand at 251.680 kb on - strand at 251.680 kb on - strand at 251.688 kb on - strand at 251.688 kb on - strand at 251.725 kb on + strand at 251.725 kb on + strand at 251.726 kb on - strand at 251.834 kb on - strand at 251.840 kb on - strand at 251.840 kb on - strand at 251.840 kb on - strand at 251.855 kb on + strand, within EX31_RS22490 at 251.855 kb on + strand, within EX31_RS22490 at 251.856 kb on - strand, within EX31_RS22490 at 251.856 kb on - strand, within EX31_RS22490 at 251.856 kb on - strand, within EX31_RS22490 at 251.861 kb on + strand, within EX31_RS22490 at 251.861 kb on + strand, within EX31_RS22490 at 251.861 kb on + strand, within EX31_RS22490 at 251.861 kb on + strand, within EX31_RS22490 at 251.896 kb on - strand, within EX31_RS22490 at 251.953 kb on + strand, within EX31_RS22490 at 251.953 kb on + strand, within EX31_RS22490 at 251.954 kb on - strand, within EX31_RS22490 at 252.005 kb on + strand at 252.005 kb on + strand at 252.005 kb on + strand at 252.005 kb on + strand at 252.005 kb on + strand at 252.006 kb on - strand at 252.006 kb on - strand at 252.006 kb on - strand at 252.007 kb on + strand at 252.007 kb on + strand at 252.007 kb on + strand at 252.008 kb on - strand at 252.008 kb on - strand at 252.008 kb on - strand at 252.008 kb on - strand at 252.008 kb on - strand at 252.018 kb on + strand at 252.019 kb on - strand at 252.019 kb on - strand at 252.019 kb on - strand at 252.024 kb on + strand at 252.024 kb on + strand at 252.024 kb on + strand at 252.024 kb on + strand at 252.024 kb on + strand at 252.025 kb on - strand at 252.040 kb on + strand at 252.040 kb on + strand at 252.040 kb on + strand at 252.040 kb on + strand at 252.041 kb on - strand at 252.041 kb on - strand at 252.041 kb on - strand at 252.041 kb on - strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.043 kb on + strand at 252.044 kb on - strand at 252.044 kb on - strand at 252.044 kb on - strand at 252.044 kb on - strand at 252.044 kb on - strand at 252.046 kb on + strand at 252.046 kb on + strand at 252.046 kb on + strand at 252.046 kb on + strand at 252.046 kb on + strand at 252.047 kb on - strand at 252.047 kb on - strand at 252.047 kb on - strand at 252.047 kb on - strand at 252.047 kb on - strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.048 kb on + strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.049 kb on - strand at 252.052 kb on + strand at 252.053 kb on - strand at 252.053 kb on - strand at 252.053 kb on - strand at 252.137 kb on + strand at 252.137 kb on + strand at 252.137 kb on + strand at 252.138 kb on - strand at 252.138 kb on - strand at 252.167 kb on + strand, within EX31_RS22495 at 252.168 kb on - strand, within EX31_RS22495 at 252.309 kb on + strand, within EX31_RS22495 at 252.309 kb on + strand, within EX31_RS22495 at 252.310 kb on - strand, within EX31_RS22495 at 252.310 kb on - strand, within EX31_RS22495 at 252.310 kb on - strand, within EX31_RS22495 at 252.313 kb on + strand, within EX31_RS22495 at 252.313 kb on + strand, within EX31_RS22495 at 252.313 kb on + strand, within EX31_RS22495 at 252.313 kb on + strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.314 kb on - strand, within EX31_RS22495 at 252.446 kb on + strand, within EX31_RS22495 at 252.617 kb on + strand at 252.617 kb on + strand at 252.617 kb on + strand at 252.618 kb on - strand at 252.640 kb on + strand at 252.640 kb on + strand at 252.650 kb on + strand at 252.662 kb on + strand at 252.662 kb on + strand at 252.662 kb on + strand at 252.662 kb on + strand at 252.663 kb on - strand at 252.663 kb on - strand at 252.674 kb on - strand at 252.697 kb on + strand at 252.698 kb on - strand at 252.698 kb on - strand at 252.712 kb on + strand at 252.712 kb on + strand at 252.712 kb on + strand at 252.721 kb on + strand at 252.735 kb on + strand at 252.735 kb on + strand at 252.736 kb on - strand at 252.763 kb on - strand at 252.765 kb on + strand at 252.765 kb on + strand at 252.765 kb on + strand at 252.765 kb on + strand at 252.765 kb on + strand at 252.766 kb on - strand at 252.766 kb on - strand at 252.766 kb on - strand at 252.773 kb on - strand at 252.846 kb on + strand, within EX31_RS22500 at 252.847 kb on - strand, within EX31_RS22500 at 252.900 kb on + strand, within EX31_RS22500 at 252.900 kb on + strand, within EX31_RS22500 at 252.901 kb on - strand, within EX31_RS22500 at 252.926 kb on + strand, within EX31_RS22500 at 252.926 kb on + strand, within EX31_RS22500 at 252.926 kb on + strand, within EX31_RS22500 at 252.927 kb on - strand, within EX31_RS22500 at 252.927 kb on - strand, within EX31_RS22500 at 252.970 kb on - strand, within EX31_RS22500 at 253.019 kb on + strand, within EX31_RS22500 at 253.019 kb on + strand, within EX31_RS22500 at 253.019 kb on + strand, within EX31_RS22500 at 253.020 kb on - strand, within EX31_RS22500 at 253.020 kb on - strand, within EX31_RS22500 at 253.032 kb on + strand, within EX31_RS22500 at 253.045 kb on + strand, within EX31_RS22500 at 253.045 kb on + strand, within EX31_RS22500 at 253.046 kb on - strand, within EX31_RS22500 at 253.046 kb on - strand, within EX31_RS22500 at 253.118 kb on + strand at 253.118 kb on + strand at 253.119 kb on - strand at 253.171 kb on - strand at 253.187 kb on + strand at 253.187 kb on + strand at 253.187 kb on + strand at 253.230 kb on + strand at 253.231 kb on - strand at 253.232 kb on + strand at 253.232 kb on + strand at 253.232 kb on + strand at 253.233 kb on - strand at 253.233 kb on - strand at 253.268 kb on + strand at 253.269 kb on - strand at 253.272 kb on + strand at 253.273 kb on - strand at 253.287 kb on + strand at 253.288 kb on - strand at 253.298 kb on + strand at 253.298 kb on + strand at 253.299 kb on - strand at 253.326 kb on + strand at 253.333 kb on + strand at 253.333 kb on + strand at 253.333 kb on + strand at 253.337 kb on - strand at 253.345 kb on + strand at 253.364 kb on + strand, within EX31_RS22505 at 253.369 kb on + strand, within EX31_RS22505 at 253.380 kb on + strand, within EX31_RS22505 at 253.381 kb on - strand, within EX31_RS22505 at 253.424 kb on + strand, within EX31_RS22505 at 253.443 kb on + strand, within EX31_RS22505 at 253.443 kb on + strand, within EX31_RS22505 at 253.601 kb on + strand, within EX31_RS22505 at 253.601 kb on + strand, within EX31_RS22505
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 251,240 + EX31_RS22485 0.84 -0.1 251,241 - EX31_RS22485 0.84 -0.1 251,295 - EX31_RS22485 0.86 -0.4 251,295 - EX31_RS22485 0.86 -0.3 251,430 + EX31_RS22485 0.90 +0.9 251,430 + EX31_RS22485 0.90 +0.1 251,431 - EX31_RS22485 0.90 +0.3 251,431 - EX31_RS22485 0.90 +0.4 251,431 - EX31_RS22485 0.90 +0.2 251,431 - EX31_RS22485 0.90 -0.8 251,464 + -0.8 251,464 + +0.0 251,465 - -0.5 251,568 + +0.5 251,568 + -0.1 251,568 + +0.3 251,568 + +0.2 251,568 + +0.7 251,568 + -0.2 251,568 + +0.4 251,569 - +0.8 251,569 - -0.2 251,569 - +0.7 251,569 - -0.9 251,569 - -0.0 251,569 - -0.8 251,569 - +0.7 251,569 - +0.0 251,569 - -0.1 251,569 - +2.1 251,595 + +0.6 251,595 + +1.1 251,595 + -0.5 251,595 + +0.1 251,597 + +0.4 251,597 + -0.5 251,598 - -0.2 251,598 - +0.3 251,598 - +0.5 251,598 - -0.6 251,627 + -0.8 251,627 + -0.1 251,628 - +0.7 251,651 + +0.2 251,651 + +0.3 251,651 + -0.1 251,651 + +0.3 251,651 + -0.6 251,651 + -0.2 251,652 - -0.4 251,679 + -0.6 251,679 + +0.2 251,679 + -0.2 251,679 + -0.1 251,679 + +0.6 251,679 + +0.2 251,679 + -0.3 251,679 + +0.1 251,680 - +0.7 251,680 - +0.3 251,688 - +0.4 251,688 - +0.2 251,725 + +0.7 251,725 + -1.5 251,726 - -0.7 251,834 - +0.8 251,840 - +0.7 251,840 - -0.0 251,840 - +0.4 251,855 + EX31_RS22490 0.13 +0.9 251,855 + EX31_RS22490 0.13 +0.7 251,856 - EX31_RS22490 0.14 -0.3 251,856 - EX31_RS22490 0.14 +0.1 251,856 - EX31_RS22490 0.14 +0.2 251,861 + EX31_RS22490 0.17 -0.5 251,861 + EX31_RS22490 0.17 -0.6 251,861 + EX31_RS22490 0.17 +0.3 251,861 + EX31_RS22490 0.17 -1.1 251,896 - EX31_RS22490 0.37 +0.2 251,953 + EX31_RS22490 0.70 -0.7 251,953 + EX31_RS22490 0.70 +1.0 251,954 - EX31_RS22490 0.70 -0.7 252,005 + +0.8 252,005 + -0.5 252,005 + -1.2 252,005 + -0.4 252,005 + -0.5 252,006 - -0.5 252,006 - -0.2 252,006 - -0.4 252,007 + -1.2 252,007 + -0.4 252,007 + -0.2 252,008 - -1.0 252,008 - -0.8 252,008 - -0.6 252,008 - +0.1 252,008 - -0.8 252,018 + +0.3 252,019 - -1.0 252,019 - -1.0 252,019 - -0.0 252,024 + -1.8 252,024 + -0.4 252,024 + -0.7 252,024 + -0.2 252,024 + -0.4 252,025 - +0.3 252,040 + -0.6 252,040 + -0.0 252,040 + -0.8 252,040 + -0.9 252,041 - +0.5 252,041 - +0.3 252,041 - -0.1 252,041 - +0.0 252,043 + -1.3 252,043 + -1.0 252,043 + +1.0 252,043 + -1.4 252,043 + +1.0 252,043 + +0.4 252,043 + -0.8 252,044 - -1.4 252,044 - +0.2 252,044 - -1.2 252,044 - +0.2 252,044 - -0.4 252,046 + -1.2 252,046 + -0.3 252,046 + +0.7 252,046 + -0.0 252,046 + -1.7 252,047 - -2.2 252,047 - -0.8 252,047 - -0.2 252,047 - +0.5 252,047 - +0.3 252,048 + +0.7 252,048 + -0.6 252,048 + -0.9 252,048 + +0.5 252,048 + -1.3 252,048 + -0.6 252,048 + +0.7 252,048 + -1.1 252,048 + -0.5 252,048 + +0.6 252,048 + -0.4 252,048 + +0.1 252,049 - -0.1 252,049 - -0.3 252,049 - +0.6 252,049 - +0.2 252,049 - +0.7 252,049 - +0.1 252,049 - -0.3 252,049 - -0.6 252,049 - +0.7 252,049 - -0.4 252,049 - -0.9 252,049 - +0.1 252,049 - -0.1 252,052 + +0.2 252,053 - +0.1 252,053 - -0.5 252,053 - +0.6 252,137 + -2.2 252,137 + +0.6 252,137 + +0.1 252,138 - +0.1 252,138 - -0.2 252,167 + EX31_RS22495 0.12 -1.2 252,168 - EX31_RS22495 0.12 +0.1 252,309 + EX31_RS22495 0.39 +0.2 252,309 + EX31_RS22495 0.39 +0.5 252,310 - EX31_RS22495 0.39 +0.6 252,310 - EX31_RS22495 0.39 -0.1 252,310 - EX31_RS22495 0.39 -0.9 252,313 + EX31_RS22495 0.39 +0.3 252,313 + EX31_RS22495 0.39 +0.3 252,313 + EX31_RS22495 0.39 +0.7 252,313 + EX31_RS22495 0.39 +0.7 252,314 - EX31_RS22495 0.40 -0.3 252,314 - EX31_RS22495 0.40 -0.7 252,314 - EX31_RS22495 0.40 +1.3 252,314 - EX31_RS22495 0.40 +0.4 252,314 - EX31_RS22495 0.40 +0.1 252,314 - EX31_RS22495 0.40 +0.4 252,314 - EX31_RS22495 0.40 +0.6 252,314 - EX31_RS22495 0.40 -0.5 252,446 + EX31_RS22495 0.64 -2.4 252,617 + -2.5 252,617 + -1.1 252,617 + -0.5 252,618 - -0.2 252,640 + -0.2 252,640 + -0.3 252,650 + +0.8 252,662 + -0.2 252,662 + +0.5 252,662 + -0.6 252,662 + -0.4 252,663 - +0.6 252,663 - +0.2 252,674 - +0.6 252,697 + -0.2 252,698 - +0.6 252,698 - -0.0 252,712 + +0.2 252,712 + +0.2 252,712 + -0.1 252,721 + -0.8 252,735 + -0.4 252,735 + -0.1 252,736 - -0.2 252,763 - +1.1 252,765 + -1.0 252,765 + -1.9 252,765 + -1.1 252,765 + +0.8 252,765 + +0.3 252,766 - -0.7 252,766 - -0.1 252,766 - -0.7 252,773 - +0.5 252,846 + EX31_RS22500 0.25 +1.8 252,847 - EX31_RS22500 0.26 -0.9 252,900 + EX31_RS22500 0.40 +0.1 252,900 + EX31_RS22500 0.40 -0.1 252,901 - EX31_RS22500 0.40 -0.0 252,926 + EX31_RS22500 0.47 -0.6 252,926 + EX31_RS22500 0.47 +0.3 252,926 + EX31_RS22500 0.47 +0.5 252,927 - EX31_RS22500 0.47 -0.5 252,927 - EX31_RS22500 0.47 -0.7 252,970 - EX31_RS22500 0.59 -1.7 253,019 + EX31_RS22500 0.72 -0.1 253,019 + EX31_RS22500 0.72 +0.6 253,019 + EX31_RS22500 0.72 +0.2 253,020 - EX31_RS22500 0.73 +0.6 253,020 - EX31_RS22500 0.73 +0.6 253,032 + EX31_RS22500 0.76 +0.3 253,045 + EX31_RS22500 0.79 +0.2 253,045 + EX31_RS22500 0.79 +0.4 253,046 - EX31_RS22500 0.80 -0.5 253,046 - EX31_RS22500 0.80 -0.9 253,118 + -0.5 253,118 + +0.0 253,119 - -0.7 253,171 - -0.0 253,187 + +1.1 253,187 + +0.7 253,187 + +0.4 253,230 + -0.1 253,231 - +0.1 253,232 + +0.1 253,232 + +0.2 253,232 + +0.7 253,233 - -0.9 253,233 - -0.1 253,268 + +0.1 253,269 - +0.8 253,272 + -0.2 253,273 - +0.5 253,287 + +0.0 253,288 - -0.1 253,298 + +1.3 253,298 + -0.6 253,299 - +0.5 253,326 + -0.1 253,333 + -0.1 253,333 + +0.6 253,333 + +0.1 253,337 - -0.2 253,345 + -0.1 253,364 + EX31_RS22505 0.11 -1.1 253,369 + EX31_RS22505 0.12 -0.0 253,380 + EX31_RS22505 0.14 -0.2 253,381 - EX31_RS22505 0.14 -0.6 253,424 + EX31_RS22505 0.22 -1.1 253,443 + EX31_RS22505 0.25 +1.1 253,443 + EX31_RS22505 0.25 -0.3 253,601 + EX31_RS22505 0.54 -0.1 253,601 + EX31_RS22505 0.54 +0.2
Or see this region's nucleotide sequence