Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15325 and EX31_RS15330 are separated by 75 nucleotides EX31_RS15330 and EX31_RS15335 are separated by 14 nucleotides EX31_RS15335 and EX31_RS15340 are separated by 171 nucleotides
EX31_RS15325: EX31_RS15325 - nitrogenase molybdenum-iron protein subunit beta, at 16,351 to 17,919
_RS15325
EX31_RS15330: EX31_RS15330 - putative nitrogen fixation protein NifT, at 17,995 to 18,210
_RS15330
EX31_RS15335: EX31_RS15335 - protein NifY, at 18,225 to 18,899
_RS15335
EX31_RS15340: EX31_RS15340 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 19,071 to 20,444
_RS15340
Position (kb) 18
19 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.455 kb on + strand, within EX31_RS15325 at 17.455 kb on + strand, within EX31_RS15325 at 17.455 kb on + strand, within EX31_RS15325 at 17.456 kb on - strand, within EX31_RS15325 at 17.456 kb on - strand, within EX31_RS15325 at 17.562 kb on + strand, within EX31_RS15325 at 17.562 kb on + strand, within EX31_RS15325 at 17.563 kb on - strand, within EX31_RS15325 at 17.563 kb on - strand, within EX31_RS15325 at 17.563 kb on - strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.611 kb on + strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.612 kb on - strand, within EX31_RS15325 at 17.652 kb on + strand, within EX31_RS15325 at 17.658 kb on + strand, within EX31_RS15325 at 17.670 kb on + strand, within EX31_RS15325 at 17.670 kb on + strand, within EX31_RS15325 at 17.670 kb on + strand, within EX31_RS15325 at 17.670 kb on + strand, within EX31_RS15325 at 17.670 kb on + strand, within EX31_RS15325 at 17.671 kb on - strand, within EX31_RS15325 at 17.671 kb on - strand, within EX31_RS15325 at 17.671 kb on - strand, within EX31_RS15325 at 17.671 kb on - strand, within EX31_RS15325 at 17.738 kb on + strand, within EX31_RS15325 at 17.738 kb on + strand, within EX31_RS15325 at 17.896 kb on + strand at 17.896 kb on + strand at 17.920 kb on + strand at 17.920 kb on + strand at 17.920 kb on + strand at 17.920 kb on + strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.921 kb on - strand at 17.994 kb on + strand at 17.994 kb on + strand at 17.994 kb on + strand at 17.994 kb on + strand at 17.995 kb on - strand at 17.995 kb on - strand at 17.995 kb on - strand at 18.006 kb on + strand at 18.021 kb on + strand, within EX31_RS15330 at 18.021 kb on + strand, within EX31_RS15330 at 18.022 kb on - strand, within EX31_RS15330 at 18.022 kb on - strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.024 kb on + strand, within EX31_RS15330 at 18.025 kb on - strand, within EX31_RS15330 at 18.034 kb on + strand, within EX31_RS15330 at 18.034 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.040 kb on + strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.041 kb on - strand, within EX31_RS15330 at 18.088 kb on - strand, within EX31_RS15330 at 18.088 kb on - strand, within EX31_RS15330 at 18.139 kb on + strand, within EX31_RS15330 at 18.139 kb on + strand, within EX31_RS15330 at 18.139 kb on + strand, within EX31_RS15330 at 18.140 kb on - strand, within EX31_RS15330 at 18.142 kb on + strand, within EX31_RS15330 at 18.142 kb on + strand, within EX31_RS15330 at 18.142 kb on + strand, within EX31_RS15330 at 18.142 kb on + strand, within EX31_RS15330 at 18.142 kb on + strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.143 kb on - strand, within EX31_RS15330 at 18.274 kb on + strand at 18.275 kb on - strand at 18.361 kb on + strand, within EX31_RS15335 at 18.361 kb on + strand, within EX31_RS15335 at 18.361 kb on + strand, within EX31_RS15335 at 18.361 kb on + strand, within EX31_RS15335 at 18.362 kb on - strand, within EX31_RS15335 at 18.455 kb on - strand, within EX31_RS15335 at 18.514 kb on + strand, within EX31_RS15335 at 18.514 kb on + strand, within EX31_RS15335 at 18.514 kb on + strand, within EX31_RS15335 at 18.514 kb on + strand, within EX31_RS15335 at 18.515 kb on - strand, within EX31_RS15335 at 18.588 kb on + strand, within EX31_RS15335 at 18.588 kb on + strand, within EX31_RS15335 at 18.589 kb on - strand, within EX31_RS15335 at 18.612 kb on + strand, within EX31_RS15335 at 18.612 kb on + strand, within EX31_RS15335 at 18.752 kb on + strand, within EX31_RS15335 at 18.752 kb on + strand, within EX31_RS15335 at 18.753 kb on - strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.755 kb on + strand, within EX31_RS15335 at 18.756 kb on - strand, within EX31_RS15335 at 18.756 kb on - strand, within EX31_RS15335 at 18.756 kb on - strand, within EX31_RS15335 at 18.756 kb on - strand, within EX31_RS15335 at 18.870 kb on - strand at 19.004 kb on + strand at 19.045 kb on + strand at 19.046 kb on - strand at 19.046 kb on - strand at 19.046 kb on - strand at 19.049 kb on + strand at 19.049 kb on + strand at 19.049 kb on + strand at 19.049 kb on + strand at 19.050 kb on - strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.119 kb on + strand at 19.120 kb on - strand at 19.121 kb on + strand at 19.122 kb on - strand at 19.122 kb on - strand at 19.122 kb on - strand at 19.122 kb on - strand at 19.237 kb on + strand, within EX31_RS15340 at 19.237 kb on + strand, within EX31_RS15340 at 19.256 kb on + strand, within EX31_RS15340 at 19.256 kb on + strand, within EX31_RS15340 at 19.257 kb on - strand, within EX31_RS15340 at 19.375 kb on + strand, within EX31_RS15340 at 19.375 kb on + strand, within EX31_RS15340 at 19.375 kb on + strand, within EX31_RS15340 at 19.375 kb on + strand, within EX31_RS15340 at 19.376 kb on - strand, within EX31_RS15340 at 19.376 kb on - strand, within EX31_RS15340 at 19.407 kb on + strand, within EX31_RS15340 at 19.427 kb on + strand, within EX31_RS15340 at 19.431 kb on + strand, within EX31_RS15340 at 19.431 kb on + strand, within EX31_RS15340 at 19.432 kb on - strand, within EX31_RS15340 at 19.432 kb on - strand, within EX31_RS15340 at 19.444 kb on + strand, within EX31_RS15340 at 19.539 kb on + strand, within EX31_RS15340 at 19.540 kb on - strand, within EX31_RS15340 at 19.685 kb on + strand, within EX31_RS15340 at 19.686 kb on - strand, within EX31_RS15340 at 19.748 kb on + strand, within EX31_RS15340 at 19.749 kb on - strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.787 kb on + strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.788 kb on - strand, within EX31_RS15340 at 19.863 kb on + strand, within EX31_RS15340 at 19.894 kb on - strand, within EX31_RS15340
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 17,455 + EX31_RS15325 0.70 +0.8 17,455 + EX31_RS15325 0.70 +0.2 17,455 + EX31_RS15325 0.70 -0.0 17,456 - EX31_RS15325 0.70 +0.0 17,456 - EX31_RS15325 0.70 +1.0 17,562 + EX31_RS15325 0.77 +0.5 17,562 + EX31_RS15325 0.77 +0.1 17,563 - EX31_RS15325 0.77 +1.2 17,563 - EX31_RS15325 0.77 -1.1 17,563 - EX31_RS15325 0.77 +0.2 17,611 + EX31_RS15325 0.80 +0.3 17,611 + EX31_RS15325 0.80 -0.7 17,611 + EX31_RS15325 0.80 +0.5 17,611 + EX31_RS15325 0.80 +0.0 17,611 + EX31_RS15325 0.80 -0.8 17,611 + EX31_RS15325 0.80 +0.2 17,611 + EX31_RS15325 0.80 -0.3 17,611 + EX31_RS15325 0.80 -0.2 17,611 + EX31_RS15325 0.80 -0.6 17,611 + EX31_RS15325 0.80 -1.5 17,611 + EX31_RS15325 0.80 -1.1 17,611 + EX31_RS15325 0.80 -1.4 17,612 - EX31_RS15325 0.80 +2.0 17,612 - EX31_RS15325 0.80 -1.2 17,612 - EX31_RS15325 0.80 +0.3 17,612 - EX31_RS15325 0.80 -0.6 17,612 - EX31_RS15325 0.80 +0.6 17,612 - EX31_RS15325 0.80 +0.9 17,612 - EX31_RS15325 0.80 -1.2 17,612 - EX31_RS15325 0.80 +1.2 17,612 - EX31_RS15325 0.80 -2.2 17,612 - EX31_RS15325 0.80 -0.6 17,652 + EX31_RS15325 0.83 -1.9 17,658 + EX31_RS15325 0.83 -0.1 17,670 + EX31_RS15325 0.84 -0.2 17,670 + EX31_RS15325 0.84 +0.2 17,670 + EX31_RS15325 0.84 -0.6 17,670 + EX31_RS15325 0.84 +0.1 17,670 + EX31_RS15325 0.84 -0.1 17,671 - EX31_RS15325 0.84 -0.2 17,671 - EX31_RS15325 0.84 -2.2 17,671 - EX31_RS15325 0.84 -0.8 17,671 - EX31_RS15325 0.84 -0.0 17,738 + EX31_RS15325 0.88 +0.1 17,738 + EX31_RS15325 0.88 -0.5 17,896 + +0.3 17,896 + +0.6 17,920 + -1.0 17,920 + +1.0 17,920 + -0.2 17,920 + +0.3 17,921 - +0.8 17,921 - +0.3 17,921 - -0.4 17,921 - +1.2 17,994 + +0.6 17,994 + +0.3 17,994 + -0.8 17,994 + +0.2 17,995 - -0.2 17,995 - +0.1 17,995 - +0.4 18,006 + +0.4 18,021 + EX31_RS15330 0.12 +0.2 18,021 + EX31_RS15330 0.12 +0.5 18,022 - EX31_RS15330 0.12 -0.5 18,022 - EX31_RS15330 0.12 +1.2 18,024 + EX31_RS15330 0.13 +0.8 18,024 + EX31_RS15330 0.13 -0.1 18,024 + EX31_RS15330 0.13 +0.2 18,024 + EX31_RS15330 0.13 +0.5 18,024 + EX31_RS15330 0.13 -0.4 18,024 + EX31_RS15330 0.13 +1.2 18,025 - EX31_RS15330 0.14 +1.0 18,034 + EX31_RS15330 0.18 -0.4 18,034 + EX31_RS15330 0.18 -0.8 18,040 + EX31_RS15330 0.21 +0.1 18,040 + EX31_RS15330 0.21 -0.6 18,040 + EX31_RS15330 0.21 +0.3 18,040 + EX31_RS15330 0.21 -1.4 18,040 + EX31_RS15330 0.21 -0.3 18,040 + EX31_RS15330 0.21 -0.1 18,040 + EX31_RS15330 0.21 -0.1 18,040 + EX31_RS15330 0.21 -0.6 18,041 - EX31_RS15330 0.21 +0.7 18,041 - EX31_RS15330 0.21 +0.8 18,041 - EX31_RS15330 0.21 -0.8 18,041 - EX31_RS15330 0.21 +0.6 18,041 - EX31_RS15330 0.21 +0.6 18,041 - EX31_RS15330 0.21 -0.2 18,041 - EX31_RS15330 0.21 +0.5 18,088 - EX31_RS15330 0.43 -1.6 18,088 - EX31_RS15330 0.43 -0.6 18,139 + EX31_RS15330 0.67 +0.7 18,139 + EX31_RS15330 0.67 +0.9 18,139 + EX31_RS15330 0.67 +0.2 18,140 - EX31_RS15330 0.67 -1.2 18,142 + EX31_RS15330 0.68 -0.0 18,142 + EX31_RS15330 0.68 -1.1 18,142 + EX31_RS15330 0.68 -2.5 18,142 + EX31_RS15330 0.68 +0.1 18,142 + EX31_RS15330 0.68 -0.3 18,143 - EX31_RS15330 0.69 -0.8 18,143 - EX31_RS15330 0.69 -1.5 18,143 - EX31_RS15330 0.69 -1.2 18,143 - EX31_RS15330 0.69 +0.2 18,143 - EX31_RS15330 0.69 +0.3 18,143 - EX31_RS15330 0.69 +0.0 18,143 - EX31_RS15330 0.69 -1.5 18,274 + +0.7 18,275 - -0.4 18,361 + EX31_RS15335 0.20 +0.7 18,361 + EX31_RS15335 0.20 -0.1 18,361 + EX31_RS15335 0.20 +0.4 18,361 + EX31_RS15335 0.20 +0.8 18,362 - EX31_RS15335 0.20 -0.6 18,455 - EX31_RS15335 0.34 -0.1 18,514 + EX31_RS15335 0.43 +0.2 18,514 + EX31_RS15335 0.43 +0.2 18,514 + EX31_RS15335 0.43 +0.2 18,514 + EX31_RS15335 0.43 +1.0 18,515 - EX31_RS15335 0.43 +0.4 18,588 + EX31_RS15335 0.54 +0.3 18,588 + EX31_RS15335 0.54 +0.3 18,589 - EX31_RS15335 0.54 +0.1 18,612 + EX31_RS15335 0.57 -0.2 18,612 + EX31_RS15335 0.57 +0.3 18,752 + EX31_RS15335 0.78 +0.1 18,752 + EX31_RS15335 0.78 +1.2 18,753 - EX31_RS15335 0.78 +0.8 18,755 + EX31_RS15335 0.79 +0.5 18,755 + EX31_RS15335 0.79 -0.5 18,755 + EX31_RS15335 0.79 +0.4 18,755 + EX31_RS15335 0.79 +0.1 18,755 + EX31_RS15335 0.79 -0.8 18,755 + EX31_RS15335 0.79 +0.7 18,755 + EX31_RS15335 0.79 -0.5 18,755 + EX31_RS15335 0.79 +0.6 18,756 - EX31_RS15335 0.79 +0.0 18,756 - EX31_RS15335 0.79 +0.4 18,756 - EX31_RS15335 0.79 -0.0 18,756 - EX31_RS15335 0.79 -1.0 18,870 - -0.6 19,004 + +0.1 19,045 + -0.3 19,046 - +1.0 19,046 - +0.2 19,046 - +0.6 19,049 + +0.4 19,049 + +0.3 19,049 + -0.4 19,049 + -0.9 19,050 - -2.1 19,119 + -0.4 19,119 + +1.5 19,119 + +0.5 19,119 + +0.2 19,119 + -0.3 19,119 + -0.5 19,119 + -0.4 19,120 - -0.2 19,121 + -0.3 19,122 - +0.8 19,122 - -1.3 19,122 - +0.6 19,122 - -0.2 19,237 + EX31_RS15340 0.12 +1.1 19,237 + EX31_RS15340 0.12 +0.5 19,256 + EX31_RS15340 0.13 +0.5 19,256 + EX31_RS15340 0.13 -0.0 19,257 - EX31_RS15340 0.14 -0.0 19,375 + EX31_RS15340 0.22 +0.2 19,375 + EX31_RS15340 0.22 -0.1 19,375 + EX31_RS15340 0.22 +0.4 19,375 + EX31_RS15340 0.22 -0.8 19,376 - EX31_RS15340 0.22 -0.3 19,376 - EX31_RS15340 0.22 +0.4 19,407 + EX31_RS15340 0.24 -0.1 19,427 + EX31_RS15340 0.26 -0.2 19,431 + EX31_RS15340 0.26 -0.3 19,431 + EX31_RS15340 0.26 +0.9 19,432 - EX31_RS15340 0.26 +0.0 19,432 - EX31_RS15340 0.26 -1.9 19,444 + EX31_RS15340 0.27 -0.5 19,539 + EX31_RS15340 0.34 -1.0 19,540 - EX31_RS15340 0.34 -0.0 19,685 + EX31_RS15340 0.45 -0.9 19,686 - EX31_RS15340 0.45 -0.1 19,748 + EX31_RS15340 0.49 -1.2 19,749 - EX31_RS15340 0.49 -0.6 19,787 + EX31_RS15340 0.52 -0.0 19,787 + EX31_RS15340 0.52 +0.1 19,787 + EX31_RS15340 0.52 -0.5 19,787 + EX31_RS15340 0.52 +0.8 19,787 + EX31_RS15340 0.52 +0.5 19,787 + EX31_RS15340 0.52 -0.4 19,787 + EX31_RS15340 0.52 +0.5 19,788 - EX31_RS15340 0.52 -1.0 19,788 - EX31_RS15340 0.52 -0.8 19,788 - EX31_RS15340 0.52 -0.3 19,788 - EX31_RS15340 0.52 -2.7 19,863 + EX31_RS15340 0.58 -0.2 19,894 - EX31_RS15340 0.60 +1.1
Or see this region's nucleotide sequence