Strain Fitness in Rahnella sp. WP5 around EX31_RS11215

Experiment: NF_with10percent_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS11210 and EX31_RS11215 are separated by 105 nucleotidesEX31_RS11215 and EX31_RS11220 are separated by 170 nucleotides EX31_RS11210: EX31_RS11210 - oxidoreductase, at 174,497 to 175,474 _RS11210 EX31_RS11215: EX31_RS11215 - TetR family transcriptional regulator, at 175,580 to 176,248 _RS11215 EX31_RS11220: EX31_RS11220 - LLM class flavin-dependent oxidoreductase, at 176,419 to 177,756 _RS11220 Position (kb) 175 176 177Strain fitness (log2 ratio) -2 -1 0 1 2at 174.622 kb on + strand, within EX31_RS11210at 174.911 kb on - strand, within EX31_RS11210at 174.918 kb on + strand, within EX31_RS11210at 174.919 kb on - strand, within EX31_RS11210at 174.928 kb on + strand, within EX31_RS11210at 174.928 kb on + strand, within EX31_RS11210at 174.928 kb on + strand, within EX31_RS11210at 174.929 kb on - strand, within EX31_RS11210at 174.929 kb on - strand, within EX31_RS11210at 174.959 kb on - strand, within EX31_RS11210at 174.959 kb on - strand, within EX31_RS11210at 174.970 kb on + strand, within EX31_RS11210at 174.970 kb on + strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.971 kb on - strand, within EX31_RS11210at 174.992 kb on - strand, within EX31_RS11210at 175.354 kb on - strand, within EX31_RS11210at 175.385 kb on + strandat 175.386 kb on - strandat 175.386 kb on - strandat 175.386 kb on - strandat 175.521 kb on + strandat 175.537 kb on + strandat 175.538 kb on - strandat 175.538 kb on - strandat 175.566 kb on + strandat 175.567 kb on - strandat 175.606 kb on + strandat 175.607 kb on - strandat 175.851 kb on - strand, within EX31_RS11215at 175.996 kb on - strand, within EX31_RS11215at 176.029 kb on + strand, within EX31_RS11215at 176.029 kb on + strand, within EX31_RS11215at 176.030 kb on - strand, within EX31_RS11215at 176.030 kb on - strand, within EX31_RS11215at 176.040 kb on + strand, within EX31_RS11215at 176.041 kb on - strand, within EX31_RS11215at 176.046 kb on + strand, within EX31_RS11215at 176.159 kb on - strand, within EX31_RS11215at 176.159 kb on - strand, within EX31_RS11215at 176.344 kb on + strandat 176.410 kb on - strandat 176.417 kb on - strandat 176.460 kb on + strandat 176.461 kb on - strandat 176.462 kb on + strandat 176.484 kb on + strandat 176.607 kb on + strand, within EX31_RS11220at 176.608 kb on - strand, within EX31_RS11220at 176.789 kb on - strand, within EX31_RS11220at 176.910 kb on - strand, within EX31_RS11220at 176.968 kb on - strand, within EX31_RS11220at 177.021 kb on - strand, within EX31_RS11220at 177.075 kb on + strand, within EX31_RS11220at 177.198 kb on + strand, within EX31_RS11220at 177.198 kb on + strand, within EX31_RS11220

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with10percent_fullN
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174,622 + EX31_RS11210 0.13 +0.2
174,911 - EX31_RS11210 0.42 -0.5
174,918 + EX31_RS11210 0.43 +0.1
174,919 - EX31_RS11210 0.43 +1.1
174,928 + EX31_RS11210 0.44 +0.0
174,928 + EX31_RS11210 0.44 -0.3
174,928 + EX31_RS11210 0.44 -0.4
174,929 - EX31_RS11210 0.44 -0.5
174,929 - EX31_RS11210 0.44 +0.8
174,959 - EX31_RS11210 0.47 +0.6
174,959 - EX31_RS11210 0.47 +1.0
174,970 + EX31_RS11210 0.48 -0.9
174,970 + EX31_RS11210 0.48 -2.0
174,971 - EX31_RS11210 0.48 +1.2
174,971 - EX31_RS11210 0.48 +0.6
174,971 - EX31_RS11210 0.48 +0.5
174,971 - EX31_RS11210 0.48 +0.8
174,971 - EX31_RS11210 0.48 +0.6
174,971 - EX31_RS11210 0.48 -0.3
174,971 - EX31_RS11210 0.48 -0.7
174,992 - EX31_RS11210 0.51 -0.5
175,354 - EX31_RS11210 0.88 +0.4
175,385 + -0.7
175,386 - +0.8
175,386 - +0.5
175,386 - +0.6
175,521 + +0.4
175,537 + +0.5
175,538 - +1.0
175,538 - +0.6
175,566 + +0.9
175,567 - +0.2
175,606 + +0.2
175,607 - +1.3
175,851 - EX31_RS11215 0.41 +0.9
175,996 - EX31_RS11215 0.62 +1.1
176,029 + EX31_RS11215 0.67 -0.6
176,029 + EX31_RS11215 0.67 -1.3
176,030 - EX31_RS11215 0.67 +0.9
176,030 - EX31_RS11215 0.67 +0.9
176,040 + EX31_RS11215 0.69 -0.7
176,041 - EX31_RS11215 0.69 +1.2
176,046 + EX31_RS11215 0.70 -1.5
176,159 - EX31_RS11215 0.87 +0.5
176,159 - EX31_RS11215 0.87 +0.5
176,344 + +1.2
176,410 - -1.8
176,417 - -0.2
176,460 + +0.0
176,461 - +0.5
176,462 + +0.4
176,484 + +0.5
176,607 + EX31_RS11220 0.14 -1.1
176,608 - EX31_RS11220 0.14 +0.7
176,789 - EX31_RS11220 0.28 -0.0
176,910 - EX31_RS11220 0.37 +0.7
176,968 - EX31_RS11220 0.41 +0.7
177,021 - EX31_RS11220 0.45 +0.9
177,075 + EX31_RS11220 0.49 +2.2
177,198 + EX31_RS11220 0.58 -1.0
177,198 + EX31_RS11220 0.58 -2.1

Or see this region's nucleotide sequence