Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS01910 and EX31_RS01915 overlap by 4 nucleotides EX31_RS01915 and EX31_RS01920 are separated by 31 nucleotides EX31_RS01920 and EX31_RS25095 are separated by 37 nucleotides
EX31_RS01910: EX31_RS01910 - lipase family protein, at 414,175 to 416,124
_RS01910
EX31_RS01915: EX31_RS01915 - hypothetical protein, at 416,121 to 416,895
_RS01915
EX31_RS01920: EX31_RS01920 - hypothetical protein, at 416,927 to 417,685
_RS01920
EX31_RS25095: EX31_RS25095 - hypothetical protein, at 417,723 to 418,470
_RS25095
Position (kb)
416
417 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 415.167 kb on + strand, within EX31_RS01910 at 415.168 kb on - strand, within EX31_RS01910 at 415.172 kb on + strand, within EX31_RS01910 at 415.172 kb on + strand, within EX31_RS01910 at 415.173 kb on - strand, within EX31_RS01910 at 415.221 kb on - strand, within EX31_RS01910 at 415.227 kb on + strand, within EX31_RS01910 at 415.231 kb on + strand, within EX31_RS01910 at 415.231 kb on + strand, within EX31_RS01910 at 415.235 kb on + strand, within EX31_RS01910 at 415.235 kb on + strand, within EX31_RS01910 at 415.235 kb on + strand, within EX31_RS01910 at 415.236 kb on - strand, within EX31_RS01910 at 415.239 kb on + strand, within EX31_RS01910 at 415.240 kb on - strand, within EX31_RS01910 at 415.277 kb on + strand, within EX31_RS01910 at 415.316 kb on - strand, within EX31_RS01910 at 415.365 kb on - strand, within EX31_RS01910 at 415.458 kb on - strand, within EX31_RS01910 at 415.475 kb on + strand, within EX31_RS01910 at 415.481 kb on + strand, within EX31_RS01910 at 415.481 kb on + strand, within EX31_RS01910 at 415.481 kb on + strand, within EX31_RS01910 at 415.509 kb on - strand, within EX31_RS01910 at 415.590 kb on + strand, within EX31_RS01910 at 415.676 kb on + strand, within EX31_RS01910 at 415.677 kb on - strand, within EX31_RS01910 at 415.677 kb on - strand, within EX31_RS01910 at 415.677 kb on - strand, within EX31_RS01910 at 415.855 kb on - strand, within EX31_RS01910 at 416.144 kb on - strand at 416.146 kb on + strand at 416.164 kb on + strand at 416.198 kb on + strand at 416.198 kb on + strand at 416.315 kb on + strand, within EX31_RS01915 at 416.358 kb on + strand, within EX31_RS01915 at 416.358 kb on + strand, within EX31_RS01915 at 416.370 kb on - strand, within EX31_RS01915 at 416.483 kb on + strand, within EX31_RS01915 at 416.556 kb on - strand, within EX31_RS01915 at 416.616 kb on + strand, within EX31_RS01915 at 416.616 kb on + strand, within EX31_RS01915 at 416.617 kb on - strand, within EX31_RS01915 at 416.723 kb on - strand, within EX31_RS01915 at 416.816 kb on + strand, within EX31_RS01915 at 416.847 kb on + strand at 416.983 kb on + strand at 416.999 kb on - strand at 417.092 kb on - strand, within EX31_RS01920 at 417.160 kb on + strand, within EX31_RS01920 at 417.193 kb on + strand, within EX31_RS01920 at 417.278 kb on + strand, within EX31_RS01920 at 417.279 kb on - strand, within EX31_RS01920 at 417.280 kb on + strand, within EX31_RS01920 at 417.280 kb on + strand, within EX31_RS01920 at 417.385 kb on - strand, within EX31_RS01920 at 417.395 kb on - strand, within EX31_RS01920 at 417.718 kb on - strand at 417.869 kb on + strand, within EX31_RS25095 at 417.869 kb on + strand, within EX31_RS25095
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 415,167 + EX31_RS01910 0.51 -0.9 415,168 - EX31_RS01910 0.51 -2.8 415,172 + EX31_RS01910 0.51 -0.6 415,172 + EX31_RS01910 0.51 -0.8 415,173 - EX31_RS01910 0.51 +2.2 415,221 - EX31_RS01910 0.54 +0.7 415,227 + EX31_RS01910 0.54 +1.1 415,231 + EX31_RS01910 0.54 +0.5 415,231 + EX31_RS01910 0.54 -0.5 415,235 + EX31_RS01910 0.54 -0.1 415,235 + EX31_RS01910 0.54 +0.7 415,235 + EX31_RS01910 0.54 -1.3 415,236 - EX31_RS01910 0.54 -0.7 415,239 + EX31_RS01910 0.55 +0.3 415,240 - EX31_RS01910 0.55 -2.3 415,277 + EX31_RS01910 0.57 +1.0 415,316 - EX31_RS01910 0.59 -1.3 415,365 - EX31_RS01910 0.61 -0.6 415,458 - EX31_RS01910 0.66 -1.5 415,475 + EX31_RS01910 0.67 -0.9 415,481 + EX31_RS01910 0.67 -2.9 415,481 + EX31_RS01910 0.67 -0.3 415,481 + EX31_RS01910 0.67 -1.9 415,509 - EX31_RS01910 0.68 -1.8 415,590 + EX31_RS01910 0.73 -0.7 415,676 + EX31_RS01910 0.77 +0.2 415,677 - EX31_RS01910 0.77 +1.2 415,677 - EX31_RS01910 0.77 -0.4 415,677 - EX31_RS01910 0.77 -0.6 415,855 - EX31_RS01910 0.86 -1.5 416,144 - -2.1 416,146 + +0.0 416,164 + +0.1 416,198 + +0.3 416,198 + -0.4 416,315 + EX31_RS01915 0.25 +0.1 416,358 + EX31_RS01915 0.31 +0.8 416,358 + EX31_RS01915 0.31 -1.5 416,370 - EX31_RS01915 0.32 -0.6 416,483 + EX31_RS01915 0.47 +0.2 416,556 - EX31_RS01915 0.56 -0.3 416,616 + EX31_RS01915 0.64 -0.3 416,616 + EX31_RS01915 0.64 +0.4 416,617 - EX31_RS01915 0.64 -0.3 416,723 - EX31_RS01915 0.78 +0.4 416,816 + EX31_RS01915 0.90 -1.7 416,847 + -1.0 416,983 + -1.9 416,999 - -2.8 417,092 - EX31_RS01920 0.22 -2.0 417,160 + EX31_RS01920 0.31 -2.0 417,193 + EX31_RS01920 0.35 -0.8 417,278 + EX31_RS01920 0.46 -1.5 417,279 - EX31_RS01920 0.46 -0.6 417,280 + EX31_RS01920 0.47 -0.2 417,280 + EX31_RS01920 0.47 -1.8 417,385 - EX31_RS01920 0.60 +1.1 417,395 - EX31_RS01920 0.62 -1.4 417,718 - -1.1 417,869 + EX31_RS25095 0.20 +1.7 417,869 + EX31_RS25095 0.20 -1.2
Or see this region's nucleotide sequence