Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS14225 and EX31_RS14230 are separated by 203 nucleotides EX31_RS14230 and EX31_RS14235 are separated by 99 nucleotides
EX31_RS14225: EX31_RS14225 - primosomal protein N', at 25,688 to 27,883
_RS14225
EX31_RS14230: EX31_RS14230 - DNA-binding transcriptional regulator CytR, at 28,087 to 29,115
_RS14230
EX31_RS14235: EX31_RS14235 - cell division protein FtsN, at 29,215 to 30,156
_RS14235
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-2
-1
0
1 at 27.908 kb on - strand at 27.958 kb on + strand at 27.959 kb on - strand at 27.968 kb on + strand at 27.968 kb on + strand at 27.968 kb on + strand at 27.968 kb on + strand at 27.969 kb on - strand at 27.969 kb on - strand at 27.972 kb on + strand at 27.972 kb on + strand at 27.973 kb on - strand at 27.973 kb on - strand at 27.997 kb on + strand at 27.997 kb on + strand at 27.997 kb on + strand at 28.012 kb on + strand at 28.012 kb on + strand at 28.012 kb on + strand at 28.012 kb on + strand at 28.013 kb on - strand at 28.013 kb on - strand at 28.175 kb on + strand at 28.175 kb on + strand at 28.278 kb on + strand, within EX31_RS14230 at 28.278 kb on + strand, within EX31_RS14230 at 28.279 kb on - strand, within EX31_RS14230 at 28.293 kb on + strand, within EX31_RS14230 at 28.293 kb on + strand, within EX31_RS14230 at 28.293 kb on + strand, within EX31_RS14230 at 28.447 kb on - strand, within EX31_RS14230 at 28.447 kb on - strand, within EX31_RS14230 at 28.487 kb on + strand, within EX31_RS14230 at 28.487 kb on + strand, within EX31_RS14230 at 28.488 kb on - strand, within EX31_RS14230 at 28.488 kb on - strand, within EX31_RS14230 at 28.488 kb on - strand, within EX31_RS14230 at 28.543 kb on - strand, within EX31_RS14230 at 28.618 kb on + strand, within EX31_RS14230 at 28.618 kb on + strand, within EX31_RS14230 at 28.618 kb on + strand, within EX31_RS14230 at 28.618 kb on + strand, within EX31_RS14230 at 28.619 kb on - strand, within EX31_RS14230 at 28.619 kb on - strand, within EX31_RS14230 at 28.619 kb on - strand, within EX31_RS14230 at 28.644 kb on + strand, within EX31_RS14230 at 28.644 kb on + strand, within EX31_RS14230 at 28.645 kb on - strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.671 kb on + strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.672 kb on - strand, within EX31_RS14230 at 28.702 kb on + strand, within EX31_RS14230 at 28.702 kb on + strand, within EX31_RS14230 at 28.702 kb on + strand, within EX31_RS14230 at 28.702 kb on + strand, within EX31_RS14230 at 28.702 kb on + strand, within EX31_RS14230 at 28.703 kb on - strand, within EX31_RS14230 at 28.703 kb on - strand, within EX31_RS14230 at 28.703 kb on - strand, within EX31_RS14230 at 28.703 kb on - strand, within EX31_RS14230 at 28.703 kb on - strand, within EX31_RS14230 at 28.771 kb on + strand, within EX31_RS14230 at 28.772 kb on - strand, within EX31_RS14230 at 28.772 kb on - strand, within EX31_RS14230 at 28.948 kb on + strand, within EX31_RS14230 at 29.012 kb on + strand, within EX31_RS14230 at 29.013 kb on - strand at 29.013 kb on - strand at 29.013 kb on - strand at 29.015 kb on + strand at 29.015 kb on + strand at 29.015 kb on + strand at 29.015 kb on + strand at 29.015 kb on + strand at 29.016 kb on - strand at 29.016 kb on - strand at 29.016 kb on - strand at 29.016 kb on - strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.018 kb on + strand at 29.019 kb on - strand at 29.019 kb on - strand at 29.019 kb on - strand at 29.019 kb on - strand at 29.019 kb on - strand at 29.019 kb on - strand at 29.031 kb on - strand at 29.031 kb on - strand at 29.031 kb on - strand at 29.031 kb on - strand at 29.070 kb on + strand at 29.071 kb on - strand at 29.125 kb on + strand at 29.125 kb on + strand at 29.125 kb on + strand at 29.125 kb on + strand at 29.126 kb on - strand at 29.143 kb on + strand at 29.144 kb on - strand at 29.170 kb on + strand at 29.632 kb on + strand, within EX31_RS14235 at 29.633 kb on - strand, within EX31_RS14235 at 29.633 kb on - strand, within EX31_RS14235 at 29.633 kb on - strand, within EX31_RS14235 at 29.635 kb on - strand, within EX31_RS14235 at 29.670 kb on + strand, within EX31_RS14235 at 29.670 kb on + strand, within EX31_RS14235 at 29.671 kb on - strand, within EX31_RS14235 at 29.704 kb on + strand, within EX31_RS14235 at 29.704 kb on + strand, within EX31_RS14235 at 29.705 kb on - strand, within EX31_RS14235 at 29.705 kb on - strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.758 kb on + strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.759 kb on - strand, within EX31_RS14235 at 29.760 kb on + strand, within EX31_RS14235 at 29.760 kb on + strand, within EX31_RS14235 at 29.781 kb on - strand, within EX31_RS14235 at 29.781 kb on - strand, within EX31_RS14235 at 30.067 kb on + strand at 30.067 kb on + strand at 30.067 kb on + strand at 30.067 kb on + strand at 30.068 kb on - strand at 30.068 kb on - strand at 30.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 27,908 - +0.3 27,958 + -0.3 27,959 - +0.3 27,968 + +0.4 27,968 + +0.1 27,968 + -0.5 27,968 + -0.6 27,969 - -0.3 27,969 - -0.1 27,972 + +0.4 27,972 + +0.8 27,973 - +0.3 27,973 - +0.1 27,997 + +0.3 27,997 + +0.0 27,997 + +0.5 28,012 + +0.3 28,012 + +0.5 28,012 + +0.1 28,012 + -0.4 28,013 - +0.8 28,013 - -0.0 28,175 + -1.8 28,175 + +0.0 28,278 + EX31_RS14230 0.19 -0.1 28,278 + EX31_RS14230 0.19 +0.6 28,279 - EX31_RS14230 0.19 +0.4 28,293 + EX31_RS14230 0.20 +0.4 28,293 + EX31_RS14230 0.20 +0.8 28,293 + EX31_RS14230 0.20 -0.2 28,447 - EX31_RS14230 0.35 -0.4 28,447 - EX31_RS14230 0.35 +0.3 28,487 + EX31_RS14230 0.39 -0.1 28,487 + EX31_RS14230 0.39 +0.9 28,488 - EX31_RS14230 0.39 -0.4 28,488 - EX31_RS14230 0.39 +0.0 28,488 - EX31_RS14230 0.39 -0.1 28,543 - EX31_RS14230 0.44 +1.1 28,618 + EX31_RS14230 0.52 -1.4 28,618 + EX31_RS14230 0.52 -0.7 28,618 + EX31_RS14230 0.52 +0.1 28,618 + EX31_RS14230 0.52 -1.0 28,619 - EX31_RS14230 0.52 +0.2 28,619 - EX31_RS14230 0.52 -0.3 28,619 - EX31_RS14230 0.52 -0.5 28,644 + EX31_RS14230 0.54 -0.7 28,644 + EX31_RS14230 0.54 -0.7 28,645 - EX31_RS14230 0.54 +0.5 28,671 + EX31_RS14230 0.57 -0.2 28,671 + EX31_RS14230 0.57 +0.0 28,671 + EX31_RS14230 0.57 +1.2 28,671 + EX31_RS14230 0.57 +0.3 28,671 + EX31_RS14230 0.57 -0.4 28,671 + EX31_RS14230 0.57 -0.2 28,671 + EX31_RS14230 0.57 +0.4 28,671 + EX31_RS14230 0.57 -0.2 28,671 + EX31_RS14230 0.57 -1.2 28,671 + EX31_RS14230 0.57 +0.3 28,672 - EX31_RS14230 0.57 +0.2 28,672 - EX31_RS14230 0.57 +0.7 28,672 - EX31_RS14230 0.57 +1.0 28,672 - EX31_RS14230 0.57 +0.2 28,672 - EX31_RS14230 0.57 +1.3 28,672 - EX31_RS14230 0.57 +0.6 28,702 + EX31_RS14230 0.60 +0.8 28,702 + EX31_RS14230 0.60 +0.3 28,702 + EX31_RS14230 0.60 +0.3 28,702 + EX31_RS14230 0.60 -0.6 28,702 + EX31_RS14230 0.60 +0.8 28,703 - EX31_RS14230 0.60 +0.0 28,703 - EX31_RS14230 0.60 +0.8 28,703 - EX31_RS14230 0.60 +0.4 28,703 - EX31_RS14230 0.60 +0.1 28,703 - EX31_RS14230 0.60 +0.3 28,771 + EX31_RS14230 0.66 +0.4 28,772 - EX31_RS14230 0.67 -0.2 28,772 - EX31_RS14230 0.67 +0.1 28,948 + EX31_RS14230 0.84 -0.3 29,012 + EX31_RS14230 0.90 -0.1 29,013 - -0.2 29,013 - +0.7 29,013 - +0.3 29,015 + -0.2 29,015 + -1.4 29,015 + +0.6 29,015 + +0.3 29,015 + -0.7 29,016 - -0.8 29,016 - -0.7 29,016 - -0.9 29,016 - -0.2 29,018 + -0.7 29,018 + +0.3 29,018 + -0.6 29,018 + +0.5 29,018 + -0.8 29,018 + -0.6 29,018 + +0.5 29,018 + -0.1 29,019 - +0.2 29,019 - +0.6 29,019 - -0.1 29,019 - -0.8 29,019 - -0.3 29,019 - +0.3 29,031 - -0.7 29,031 - +0.1 29,031 - -1.5 29,031 - +0.2 29,070 + +0.5 29,071 - +0.9 29,125 + -0.1 29,125 + +0.7 29,125 + -0.0 29,125 + +0.6 29,126 - +0.3 29,143 + +0.3 29,144 - -1.7 29,170 + +0.1 29,632 + EX31_RS14235 0.44 -1.2 29,633 - EX31_RS14235 0.44 -0.8 29,633 - EX31_RS14235 0.44 -0.1 29,633 - EX31_RS14235 0.44 +0.6 29,635 - EX31_RS14235 0.45 -2.0 29,670 + EX31_RS14235 0.48 -1.0 29,670 + EX31_RS14235 0.48 -0.8 29,671 - EX31_RS14235 0.48 -1.2 29,704 + EX31_RS14235 0.52 -1.3 29,704 + EX31_RS14235 0.52 -1.1 29,705 - EX31_RS14235 0.52 +0.4 29,705 - EX31_RS14235 0.52 -1.2 29,758 + EX31_RS14235 0.58 -0.1 29,758 + EX31_RS14235 0.58 -0.3 29,758 + EX31_RS14235 0.58 -0.5 29,758 + EX31_RS14235 0.58 -1.2 29,758 + EX31_RS14235 0.58 -1.0 29,758 + EX31_RS14235 0.58 -0.5 29,758 + EX31_RS14235 0.58 -0.7 29,758 + EX31_RS14235 0.58 +0.3 29,758 + EX31_RS14235 0.58 -1.0 29,758 + EX31_RS14235 0.58 -1.5 29,758 + EX31_RS14235 0.58 -0.6 29,758 + EX31_RS14235 0.58 +0.3 29,758 + EX31_RS14235 0.58 -0.7 29,758 + EX31_RS14235 0.58 -0.5 29,758 + EX31_RS14235 0.58 -1.5 29,759 - EX31_RS14235 0.58 -0.6 29,759 - EX31_RS14235 0.58 -1.0 29,759 - EX31_RS14235 0.58 -1.6 29,759 - EX31_RS14235 0.58 +0.5 29,759 - EX31_RS14235 0.58 -1.4 29,759 - EX31_RS14235 0.58 -0.1 29,759 - EX31_RS14235 0.58 -0.3 29,759 - EX31_RS14235 0.58 -0.4 29,759 - EX31_RS14235 0.58 -0.2 29,759 - EX31_RS14235 0.58 -1.2 29,759 - EX31_RS14235 0.58 -1.8 29,759 - EX31_RS14235 0.58 -1.0 29,759 - EX31_RS14235 0.58 -1.3 29,759 - EX31_RS14235 0.58 -0.2 29,759 - EX31_RS14235 0.58 -0.5 29,759 - EX31_RS14235 0.58 -1.0 29,760 + EX31_RS14235 0.58 -0.1 29,760 + EX31_RS14235 0.58 -0.5 29,781 - EX31_RS14235 0.60 -0.7 29,781 - EX31_RS14235 0.60 -0.6 30,067 + -0.5 30,067 + -0.9 30,067 + -0.7 30,067 + -0.3 30,068 - -0.1 30,068 - +0.4 30,099 - -0.7
Or see this region's nucleotide sequence