Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS07495 and EX31_RS07500 overlap by 4 nucleotides EX31_RS07500 and EX31_RS07505 are separated by 1 nucleotides EX31_RS07505 and EX31_RS07510 overlap by 4 nucleotides
EX31_RS07495: EX31_RS07495 - ABC transporter substrate-binding protein, at 313,202 to 314,695
_RS07495
EX31_RS07500: EX31_RS07500 - ABC transporter permease, at 314,692 to 315,537
_RS07500
EX31_RS07505: EX31_RS07505 - ABC transporter permease, at 315,539 to 316,453
_RS07505
EX31_RS07510: EX31_RS07510 - M20/M25/M40 family metallo-hydrolase, at 316,450 to 317,832
_RS07510
Position (kb) 314
315
316 Strain fitness (log2 ratio) -2
-1
0
1 at 313.748 kb on + strand, within EX31_RS07495 at 313.748 kb on + strand, within EX31_RS07495 at 313.749 kb on - strand, within EX31_RS07495 at 313.765 kb on + strand, within EX31_RS07495 at 313.806 kb on - strand, within EX31_RS07495 at 313.896 kb on + strand, within EX31_RS07495 at 313.897 kb on - strand, within EX31_RS07495 at 313.897 kb on - strand, within EX31_RS07495 at 313.897 kb on - strand, within EX31_RS07495 at 313.897 kb on - strand, within EX31_RS07495 at 313.994 kb on + strand, within EX31_RS07495 at 314.091 kb on + strand, within EX31_RS07495 at 314.256 kb on - strand, within EX31_RS07495 at 314.256 kb on - strand, within EX31_RS07495 at 314.258 kb on - strand, within EX31_RS07495 at 314.258 kb on - strand, within EX31_RS07495 at 314.348 kb on + strand, within EX31_RS07495 at 314.348 kb on + strand, within EX31_RS07495 at 314.349 kb on - strand, within EX31_RS07495 at 314.387 kb on + strand, within EX31_RS07495 at 314.388 kb on - strand, within EX31_RS07495 at 314.388 kb on - strand, within EX31_RS07495 at 314.388 kb on - strand, within EX31_RS07495 at 314.465 kb on + strand, within EX31_RS07495 at 314.619 kb on - strand at 314.774 kb on + strand at 314.775 kb on - strand at 314.967 kb on - strand, within EX31_RS07500 at 315.012 kb on + strand, within EX31_RS07500 at 315.130 kb on - strand, within EX31_RS07500 at 315.163 kb on + strand, within EX31_RS07500 at 315.163 kb on + strand, within EX31_RS07500 at 315.418 kb on + strand, within EX31_RS07500 at 315.418 kb on + strand, within EX31_RS07500 at 315.418 kb on + strand, within EX31_RS07500 at 315.418 kb on + strand, within EX31_RS07500 at 315.418 kb on + strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.419 kb on - strand, within EX31_RS07500 at 315.420 kb on + strand, within EX31_RS07500 at 315.421 kb on - strand, within EX31_RS07500 at 315.421 kb on - strand, within EX31_RS07500 at 315.548 kb on + strand at 315.548 kb on + strand at 315.549 kb on - strand at 315.549 kb on - strand at 315.550 kb on + strand at 315.551 kb on - strand at 315.754 kb on - strand, within EX31_RS07505 at 315.900 kb on + strand, within EX31_RS07505 at 315.901 kb on - strand, within EX31_RS07505 at 315.903 kb on - strand, within EX31_RS07505 at 315.903 kb on - strand, within EX31_RS07505 at 316.082 kb on + strand, within EX31_RS07505 at 316.085 kb on + strand, within EX31_RS07505 at 316.085 kb on + strand, within EX31_RS07505 at 316.086 kb on - strand, within EX31_RS07505 at 316.086 kb on - strand, within EX31_RS07505 at 316.145 kb on + strand, within EX31_RS07505 at 316.145 kb on + strand, within EX31_RS07505 at 316.145 kb on + strand, within EX31_RS07505 at 316.145 kb on + strand, within EX31_RS07505 at 316.145 kb on + strand, within EX31_RS07505 at 316.146 kb on - strand, within EX31_RS07505 at 316.207 kb on - strand, within EX31_RS07505 at 316.421 kb on + strand at 316.421 kb on + strand at 316.422 kb on - strand at 316.422 kb on - strand at 316.422 kb on - strand at 316.422 kb on - strand at 316.428 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 313,748 + EX31_RS07495 0.37 -0.6 313,748 + EX31_RS07495 0.37 +0.7 313,749 - EX31_RS07495 0.37 -1.3 313,765 + EX31_RS07495 0.38 +0.6 313,806 - EX31_RS07495 0.40 -0.4 313,896 + EX31_RS07495 0.46 +0.9 313,897 - EX31_RS07495 0.47 -0.9 313,897 - EX31_RS07495 0.47 +0.5 313,897 - EX31_RS07495 0.47 -0.8 313,897 - EX31_RS07495 0.47 -0.4 313,994 + EX31_RS07495 0.53 -0.3 314,091 + EX31_RS07495 0.60 +0.1 314,256 - EX31_RS07495 0.71 -2.6 314,256 - EX31_RS07495 0.71 -1.6 314,258 - EX31_RS07495 0.71 -0.2 314,258 - EX31_RS07495 0.71 +1.5 314,348 + EX31_RS07495 0.77 -1.0 314,348 + EX31_RS07495 0.77 +1.5 314,349 - EX31_RS07495 0.77 +0.1 314,387 + EX31_RS07495 0.79 -0.0 314,388 - EX31_RS07495 0.79 -0.5 314,388 - EX31_RS07495 0.79 -0.3 314,388 - EX31_RS07495 0.79 +0.9 314,465 + EX31_RS07495 0.85 +0.1 314,619 - -0.8 314,774 + -0.8 314,775 - -0.1 314,967 - EX31_RS07500 0.33 -2.5 315,012 + EX31_RS07500 0.38 -1.8 315,130 - EX31_RS07500 0.52 -0.4 315,163 + EX31_RS07500 0.56 +1.0 315,163 + EX31_RS07500 0.56 -0.5 315,418 + EX31_RS07500 0.86 +0.4 315,418 + EX31_RS07500 0.86 +0.4 315,418 + EX31_RS07500 0.86 -0.9 315,418 + EX31_RS07500 0.86 +1.5 315,418 + EX31_RS07500 0.86 +0.3 315,419 - EX31_RS07500 0.86 +0.6 315,419 - EX31_RS07500 0.86 +0.4 315,419 - EX31_RS07500 0.86 -1.7 315,419 - EX31_RS07500 0.86 -0.2 315,419 - EX31_RS07500 0.86 -0.7 315,419 - EX31_RS07500 0.86 -0.6 315,419 - EX31_RS07500 0.86 +0.6 315,419 - EX31_RS07500 0.86 +0.6 315,420 + EX31_RS07500 0.86 -2.6 315,421 - EX31_RS07500 0.86 -1.1 315,421 - EX31_RS07500 0.86 +0.0 315,548 + -1.1 315,548 + +0.1 315,549 - +0.4 315,549 - -0.1 315,550 + -0.7 315,551 - -0.2 315,754 - EX31_RS07505 0.23 +0.4 315,900 + EX31_RS07505 0.39 -1.1 315,901 - EX31_RS07505 0.40 -1.0 315,903 - EX31_RS07505 0.40 +0.4 315,903 - EX31_RS07505 0.40 -1.3 316,082 + EX31_RS07505 0.59 +0.2 316,085 + EX31_RS07505 0.60 +0.7 316,085 + EX31_RS07505 0.60 +1.3 316,086 - EX31_RS07505 0.60 -0.3 316,086 - EX31_RS07505 0.60 -0.7 316,145 + EX31_RS07505 0.66 +1.5 316,145 + EX31_RS07505 0.66 +0.5 316,145 + EX31_RS07505 0.66 -1.0 316,145 + EX31_RS07505 0.66 +0.3 316,145 + EX31_RS07505 0.66 -0.7 316,146 - EX31_RS07505 0.66 -0.2 316,207 - EX31_RS07505 0.73 -0.4 316,421 + +0.8 316,421 + +1.2 316,422 - -1.0 316,422 - -1.4 316,422 - +1.6 316,422 - -0.7 316,428 + -0.1
Or see this region's nucleotide sequence