Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS00805 and EX31_RS00810 are separated by 2 nucleotides EX31_RS00810 and EX31_RS00815 are separated by 246 nucleotides
EX31_RS00805: EX31_RS00805 - translesion error-prone DNA polymerase V subunit UmuC, at 175,916 to 177,178
_RS00805
EX31_RS00810: EX31_RS00810 - translesion error-prone DNA polymerase V autoproteolytic subunit, at 177,181 to 177,600
_RS00810
EX31_RS00815: EX31_RS00815 - alpha/beta hydrolase, at 177,847 to 178,860
_RS00815
Position (kb) 177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 176.233 kb on + strand, within EX31_RS00805 at 176.247 kb on - strand, within EX31_RS00805 at 176.272 kb on - strand, within EX31_RS00805 at 176.275 kb on - strand, within EX31_RS00805 at 176.344 kb on + strand, within EX31_RS00805 at 176.345 kb on - strand, within EX31_RS00805 at 176.432 kb on - strand, within EX31_RS00805 at 176.578 kb on + strand, within EX31_RS00805 at 176.582 kb on - strand, within EX31_RS00805 at 176.647 kb on + strand, within EX31_RS00805 at 176.736 kb on - strand, within EX31_RS00805 at 176.798 kb on + strand, within EX31_RS00805 at 176.804 kb on - strand, within EX31_RS00805 at 177.034 kb on + strand, within EX31_RS00805 at 177.281 kb on - strand, within EX31_RS00810 at 177.286 kb on + strand, within EX31_RS00810 at 177.287 kb on - strand, within EX31_RS00810 at 177.287 kb on - strand, within EX31_RS00810 at 177.300 kb on + strand, within EX31_RS00810 at 177.359 kb on - strand, within EX31_RS00810 at 177.386 kb on - strand, within EX31_RS00810 at 177.420 kb on + strand, within EX31_RS00810 at 177.421 kb on - strand, within EX31_RS00810 at 177.421 kb on - strand, within EX31_RS00810 at 177.421 kb on - strand, within EX31_RS00810 at 177.421 kb on - strand, within EX31_RS00810 at 177.446 kb on + strand, within EX31_RS00810 at 177.446 kb on + strand, within EX31_RS00810 at 177.446 kb on + strand, within EX31_RS00810 at 177.446 kb on + strand, within EX31_RS00810 at 177.447 kb on - strand, within EX31_RS00810 at 177.503 kb on + strand, within EX31_RS00810 at 177.617 kb on + strand at 177.728 kb on - strand at 177.784 kb on - strand at 177.784 kb on - strand at 177.784 kb on - strand at 177.784 kb on - strand at 177.833 kb on - strand at 177.835 kb on + strand at 177.836 kb on - strand at 177.914 kb on + strand at 177.914 kb on + strand at 177.914 kb on + strand at 177.915 kb on - strand at 178.131 kb on + strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.132 kb on - strand, within EX31_RS00815 at 178.197 kb on + strand, within EX31_RS00815 at 178.197 kb on + strand, within EX31_RS00815 at 178.198 kb on - strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.328 kb on + strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.329 kb on - strand, within EX31_RS00815 at 178.331 kb on + strand, within EX31_RS00815 at 178.331 kb on + strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.332 kb on - strand, within EX31_RS00815 at 178.333 kb on + strand, within EX31_RS00815 at 178.333 kb on + strand, within EX31_RS00815 at 178.334 kb on - strand, within EX31_RS00815 at 178.443 kb on - strand, within EX31_RS00815 at 178.543 kb on - strand, within EX31_RS00815 at 178.549 kb on - strand, within EX31_RS00815
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 176,233 + EX31_RS00805 0.25 +0.2 176,247 - EX31_RS00805 0.26 +0.6 176,272 - EX31_RS00805 0.28 +1.7 176,275 - EX31_RS00805 0.28 -1.1 176,344 + EX31_RS00805 0.34 -0.4 176,345 - EX31_RS00805 0.34 -1.0 176,432 - EX31_RS00805 0.41 +0.5 176,578 + EX31_RS00805 0.52 +0.7 176,582 - EX31_RS00805 0.53 -0.4 176,647 + EX31_RS00805 0.58 -0.9 176,736 - EX31_RS00805 0.65 +0.2 176,798 + EX31_RS00805 0.70 -2.1 176,804 - EX31_RS00805 0.70 +0.9 177,034 + EX31_RS00805 0.89 +0.2 177,281 - EX31_RS00810 0.24 -0.8 177,286 + EX31_RS00810 0.25 +0.6 177,287 - EX31_RS00810 0.25 +1.1 177,287 - EX31_RS00810 0.25 -1.9 177,300 + EX31_RS00810 0.28 -0.4 177,359 - EX31_RS00810 0.42 -0.9 177,386 - EX31_RS00810 0.49 -0.8 177,420 + EX31_RS00810 0.57 -0.4 177,421 - EX31_RS00810 0.57 -0.9 177,421 - EX31_RS00810 0.57 -0.6 177,421 - EX31_RS00810 0.57 -0.7 177,421 - EX31_RS00810 0.57 -1.3 177,446 + EX31_RS00810 0.63 -0.0 177,446 + EX31_RS00810 0.63 -0.7 177,446 + EX31_RS00810 0.63 -0.7 177,446 + EX31_RS00810 0.63 +0.3 177,447 - EX31_RS00810 0.63 -0.4 177,503 + EX31_RS00810 0.77 -1.6 177,617 + +0.1 177,728 - -0.6 177,784 - -0.4 177,784 - +0.7 177,784 - +0.6 177,784 - -0.4 177,833 - -1.9 177,835 + -0.8 177,836 - +0.9 177,914 + -1.4 177,914 + +0.3 177,914 + -1.1 177,915 - -0.3 178,131 + EX31_RS00815 0.28 -0.3 178,132 - EX31_RS00815 0.28 +0.1 178,132 - EX31_RS00815 0.28 -0.6 178,132 - EX31_RS00815 0.28 +0.2 178,132 - EX31_RS00815 0.28 +0.3 178,197 + EX31_RS00815 0.35 -0.0 178,197 + EX31_RS00815 0.35 -1.0 178,198 - EX31_RS00815 0.35 -0.1 178,328 + EX31_RS00815 0.47 -0.0 178,328 + EX31_RS00815 0.47 -1.1 178,328 + EX31_RS00815 0.47 +0.3 178,328 + EX31_RS00815 0.47 +2.1 178,328 + EX31_RS00815 0.47 -1.1 178,328 + EX31_RS00815 0.47 -2.9 178,329 - EX31_RS00815 0.48 +0.1 178,329 - EX31_RS00815 0.48 +0.3 178,329 - EX31_RS00815 0.48 -0.6 178,329 - EX31_RS00815 0.48 -0.8 178,331 + EX31_RS00815 0.48 +0.2 178,331 + EX31_RS00815 0.48 -0.5 178,332 - EX31_RS00815 0.48 -0.8 178,332 - EX31_RS00815 0.48 +1.4 178,332 - EX31_RS00815 0.48 -1.5 178,332 - EX31_RS00815 0.48 -0.7 178,332 - EX31_RS00815 0.48 +0.1 178,332 - EX31_RS00815 0.48 -0.1 178,333 + EX31_RS00815 0.48 -0.6 178,333 + EX31_RS00815 0.48 +1.3 178,334 - EX31_RS00815 0.48 -1.8 178,443 - EX31_RS00815 0.59 -1.8 178,543 - EX31_RS00815 0.69 -0.7 178,549 - EX31_RS00815 0.69 -0.7
Or see this region's nucleotide sequence