Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02125 and EX31_RS02130 overlap by 4 nucleotides EX31_RS02130 and EX31_RS02135 overlap by 8 nucleotides EX31_RS02135 and EX31_RS02140 are separated by 264 nucleotides EX31_RS02140 and EX31_RS02145 are separated by 31 nucleotides
EX31_RS02125: EX31_RS02125 - helix-turn-helix transcriptional regulator, at 465,344 to 466,054
_RS02125
EX31_RS02130: EX31_RS02130 - amino acid-binding protein, at 466,051 to 466,443
_RS02130
EX31_RS02135: EX31_RS02135 - antibiotic biosynthesis monooxygenase, at 466,436 to 466,771
_RS02135
EX31_RS02140: EX31_RS02140 - DUF1852 domain-containing protein, at 467,036 to 468,019
_RS02140
EX31_RS02145: EX31_RS02145 - methionine synthase, at 468,051 to 469,082
_RS02145
Position (kb) 467
468
469 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 466.119 kb on + strand, within EX31_RS02130 at 466.120 kb on - strand, within EX31_RS02130 at 466.142 kb on + strand, within EX31_RS02130 at 466.143 kb on - strand, within EX31_RS02130 at 466.143 kb on - strand, within EX31_RS02130 at 466.148 kb on - strand, within EX31_RS02130 at 466.303 kb on + strand, within EX31_RS02130 at 466.304 kb on - strand, within EX31_RS02130 at 466.384 kb on - strand, within EX31_RS02130 at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.440 kb on - strand at 466.483 kb on - strand, within EX31_RS02135 at 466.483 kb on - strand, within EX31_RS02135 at 466.494 kb on - strand, within EX31_RS02135 at 466.508 kb on + strand, within EX31_RS02135 at 466.516 kb on - strand, within EX31_RS02135 at 466.666 kb on + strand, within EX31_RS02135 at 466.666 kb on + strand, within EX31_RS02135 at 466.804 kb on - strand at 466.853 kb on - strand at 467.032 kb on + strand at 467.087 kb on + strand at 467.088 kb on - strand at 467.090 kb on - strand at 467.104 kb on + strand at 467.333 kb on - strand, within EX31_RS02140 at 467.434 kb on + strand, within EX31_RS02140 at 467.434 kb on + strand, within EX31_RS02140 at 467.582 kb on + strand, within EX31_RS02140 at 467.582 kb on + strand, within EX31_RS02140 at 467.586 kb on - strand, within EX31_RS02140 at 467.599 kb on + strand, within EX31_RS02140 at 467.657 kb on + strand, within EX31_RS02140 at 467.770 kb on + strand, within EX31_RS02140 at 467.825 kb on + strand, within EX31_RS02140 at 467.826 kb on - strand, within EX31_RS02140 at 467.833 kb on + strand, within EX31_RS02140 at 467.851 kb on - strand, within EX31_RS02140 at 467.889 kb on + strand, within EX31_RS02140 at 467.903 kb on + strand, within EX31_RS02140 at 467.921 kb on - strand at 468.036 kb on + strand at 468.037 kb on - strand at 468.215 kb on + strand, within EX31_RS02145 at 468.420 kb on - strand, within EX31_RS02145 at 468.467 kb on + strand, within EX31_RS02145 at 468.475 kb on - strand, within EX31_RS02145 at 468.486 kb on + strand, within EX31_RS02145 at 468.487 kb on - strand, within EX31_RS02145 at 468.488 kb on + strand, within EX31_RS02145 at 468.488 kb on + strand, within EX31_RS02145
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 466,119 + EX31_RS02130 0.17 +0.2 466,120 - EX31_RS02130 0.18 -0.6 466,142 + EX31_RS02130 0.23 +0.2 466,143 - EX31_RS02130 0.23 -0.6 466,143 - EX31_RS02130 0.23 -0.0 466,148 - EX31_RS02130 0.25 -0.5 466,303 + EX31_RS02130 0.64 +0.3 466,304 - EX31_RS02130 0.64 +0.4 466,384 - EX31_RS02130 0.85 +0.9 466,440 - +1.4 466,440 - +1.0 466,440 - +0.7 466,440 - +0.0 466,440 - -0.5 466,483 - EX31_RS02135 0.14 +0.7 466,483 - EX31_RS02135 0.14 -0.6 466,494 - EX31_RS02135 0.17 +1.1 466,508 + EX31_RS02135 0.21 -1.2 466,516 - EX31_RS02135 0.24 +0.7 466,666 + EX31_RS02135 0.68 -0.4 466,666 + EX31_RS02135 0.68 -2.2 466,804 - +0.4 466,853 - +0.7 467,032 + -0.9 467,087 + +0.7 467,088 - -1.3 467,090 - +0.2 467,104 + +0.8 467,333 - EX31_RS02140 0.30 -0.1 467,434 + EX31_RS02140 0.40 +0.9 467,434 + EX31_RS02140 0.40 -0.5 467,582 + EX31_RS02140 0.55 +0.2 467,582 + EX31_RS02140 0.55 -0.7 467,586 - EX31_RS02140 0.56 -0.3 467,599 + EX31_RS02140 0.57 -1.5 467,657 + EX31_RS02140 0.63 -1.1 467,770 + EX31_RS02140 0.75 +0.3 467,825 + EX31_RS02140 0.80 +0.6 467,826 - EX31_RS02140 0.80 -0.5 467,833 + EX31_RS02140 0.81 +0.3 467,851 - EX31_RS02140 0.83 -1.9 467,889 + EX31_RS02140 0.87 +1.8 467,903 + EX31_RS02140 0.88 +0.3 467,921 - +1.0 468,036 + +0.2 468,037 - +0.1 468,215 + EX31_RS02145 0.16 -0.6 468,420 - EX31_RS02145 0.36 -0.6 468,467 + EX31_RS02145 0.40 +0.3 468,475 - EX31_RS02145 0.41 -2.8 468,486 + EX31_RS02145 0.42 -0.0 468,487 - EX31_RS02145 0.42 -3.2 468,488 + EX31_RS02145 0.42 -0.4 468,488 + EX31_RS02145 0.42 -1.1
Or see this region's nucleotide sequence