Strain Fitness in Rahnella sp. WP5 around EX31_RS18435

Experiment: NF_with10percent_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS18425 and EX31_RS18430 are separated by 44 nucleotidesEX31_RS18430 and EX31_RS18435 are separated by 206 nucleotidesEX31_RS18435 and EX31_RS18440 are separated by 2 nucleotidesEX31_RS18440 and EX31_RS18445 overlap by 1 nucleotidesEX31_RS18445 and EX31_RS18450 are separated by 59 nucleotides EX31_RS18425: EX31_RS18425 - replication endonuclease, at 258,029 to 260,056 _RS18425 EX31_RS18430: EX31_RS18430 - hypothetical protein, at 260,101 to 260,358 _RS18430 EX31_RS18435: EX31_RS18435 - DUF5405 family protein, at 260,565 to 260,846 _RS18435 EX31_RS18440: EX31_RS18440 - TraR/DksA family transcriptional regulator, at 260,849 to 261,067 _RS18440 EX31_RS18445: EX31_RS18445 - DUF2732 domain-containing protein, at 261,067 to 261,309 _RS18445 EX31_RS18450: EX31_RS18450 - hypothetical protein, at 261,369 to 261,869 _RS18450 Position (kb) 260 261Strain fitness (log2 ratio) -3 -2 -1 0 1at 259.573 kb on + strand, within EX31_RS18425at 259.574 kb on - strand, within EX31_RS18425at 259.599 kb on + strand, within EX31_RS18425at 259.631 kb on - strand, within EX31_RS18425at 259.677 kb on + strand, within EX31_RS18425at 259.677 kb on + strand, within EX31_RS18425at 259.678 kb on - strand, within EX31_RS18425at 259.700 kb on + strand, within EX31_RS18425at 259.708 kb on + strand, within EX31_RS18425at 259.722 kb on - strand, within EX31_RS18425at 259.734 kb on + strand, within EX31_RS18425at 259.734 kb on + strand, within EX31_RS18425at 259.734 kb on + strand, within EX31_RS18425at 259.735 kb on - strand, within EX31_RS18425at 259.735 kb on - strand, within EX31_RS18425at 259.735 kb on - strand, within EX31_RS18425at 259.735 kb on - strand, within EX31_RS18425at 259.735 kb on - strand, within EX31_RS18425at 259.756 kb on + strand, within EX31_RS18425at 259.756 kb on + strand, within EX31_RS18425at 259.757 kb on - strand, within EX31_RS18425at 259.757 kb on - strand, within EX31_RS18425at 259.757 kb on - strand, within EX31_RS18425at 259.794 kb on + strand, within EX31_RS18425at 259.829 kb on + strand, within EX31_RS18425at 259.833 kb on + strand, within EX31_RS18425at 259.833 kb on + strand, within EX31_RS18425at 259.834 kb on - strand, within EX31_RS18425at 259.834 kb on - strand, within EX31_RS18425at 259.839 kb on + strand, within EX31_RS18425at 259.862 kb on + strandat 259.863 kb on - strandat 259.863 kb on - strandat 259.892 kb on + strandat 259.927 kb on + strandat 260.049 kb on + strandat 260.049 kb on + strandat 260.059 kb on + strandat 260.063 kb on - strandat 260.153 kb on + strand, within EX31_RS18430at 260.172 kb on - strand, within EX31_RS18430at 260.174 kb on + strand, within EX31_RS18430at 260.174 kb on + strand, within EX31_RS18430at 260.174 kb on + strand, within EX31_RS18430at 260.174 kb on + strand, within EX31_RS18430at 260.174 kb on + strand, within EX31_RS18430at 260.175 kb on - strand, within EX31_RS18430at 260.175 kb on - strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.202 kb on + strand, within EX31_RS18430at 260.260 kb on + strand, within EX31_RS18430at 260.260 kb on + strand, within EX31_RS18430at 260.307 kb on + strand, within EX31_RS18430at 260.307 kb on + strand, within EX31_RS18430at 260.308 kb on - strand, within EX31_RS18430at 260.311 kb on + strand, within EX31_RS18430at 260.311 kb on + strand, within EX31_RS18430at 260.311 kb on + strand, within EX31_RS18430at 260.312 kb on - strand, within EX31_RS18430at 260.319 kb on + strand, within EX31_RS18430at 260.367 kb on + strandat 260.385 kb on - strandat 260.407 kb on + strandat 260.407 kb on + strandat 260.489 kb on + strandat 260.490 kb on - strandat 260.490 kb on - strandat 260.574 kb on - strandat 260.582 kb on + strandat 260.583 kb on - strandat 260.653 kb on - strand, within EX31_RS18435at 260.698 kb on - strand, within EX31_RS18435at 260.698 kb on - strand, within EX31_RS18435at 260.719 kb on + strand, within EX31_RS18435at 260.719 kb on + strand, within EX31_RS18435at 260.719 kb on + strand, within EX31_RS18435at 260.719 kb on + strand, within EX31_RS18435at 260.720 kb on - strand, within EX31_RS18435at 260.720 kb on - strand, within EX31_RS18435at 260.720 kb on - strand, within EX31_RS18435at 260.720 kb on - strand, within EX31_RS18435at 260.733 kb on + strand, within EX31_RS18435at 260.734 kb on - strand, within EX31_RS18435at 260.734 kb on - strand, within EX31_RS18435at 260.738 kb on - strand, within EX31_RS18435at 260.812 kb on - strand, within EX31_RS18435at 260.850 kb on + strandat 260.850 kb on + strandat 260.989 kb on + strand, within EX31_RS18440at 260.990 kb on - strand, within EX31_RS18440at 260.990 kb on - strand, within EX31_RS18440at 260.995 kb on + strand, within EX31_RS18440at 260.995 kb on + strand, within EX31_RS18440at 260.996 kb on - strand, within EX31_RS18440at 261.008 kb on + strand, within EX31_RS18440at 261.008 kb on + strand, within EX31_RS18440at 261.068 kb on + strandat 261.069 kb on - strandat 261.069 kb on - strandat 261.069 kb on - strandat 261.128 kb on - strand, within EX31_RS18445at 261.174 kb on + strand, within EX31_RS18445at 261.179 kb on - strand, within EX31_RS18445at 261.245 kb on - strand, within EX31_RS18445at 261.370 kb on + strandat 261.371 kb on - strandat 261.379 kb on + strandat 261.402 kb on + strandat 261.406 kb on - strandat 261.567 kb on + strand, within EX31_RS18450at 261.606 kb on - strand, within EX31_RS18450at 261.654 kb on - strand, within EX31_RS18450at 261.763 kb on + strand, within EX31_RS18450at 261.844 kb on - strandat 261.844 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with10percent_fullN
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259,573 + EX31_RS18425 0.76 +0.2
259,574 - EX31_RS18425 0.76 +0.3
259,599 + EX31_RS18425 0.77 -0.6
259,631 - EX31_RS18425 0.79 +0.2
259,677 + EX31_RS18425 0.81 +0.3
259,677 + EX31_RS18425 0.81 -0.5
259,678 - EX31_RS18425 0.81 +0.1
259,700 + EX31_RS18425 0.82 -1.7
259,708 + EX31_RS18425 0.83 -0.3
259,722 - EX31_RS18425 0.83 +1.6
259,734 + EX31_RS18425 0.84 +1.0
259,734 + EX31_RS18425 0.84 -0.2
259,734 + EX31_RS18425 0.84 -0.6
259,735 - EX31_RS18425 0.84 -0.0
259,735 - EX31_RS18425 0.84 -0.7
259,735 - EX31_RS18425 0.84 +0.2
259,735 - EX31_RS18425 0.84 +0.3
259,735 - EX31_RS18425 0.84 -0.9
259,756 + EX31_RS18425 0.85 -0.1
259,756 + EX31_RS18425 0.85 -0.0
259,757 - EX31_RS18425 0.85 +0.3
259,757 - EX31_RS18425 0.85 +0.3
259,757 - EX31_RS18425 0.85 +0.1
259,794 + EX31_RS18425 0.87 -1.0
259,829 + EX31_RS18425 0.89 -0.4
259,833 + EX31_RS18425 0.89 +1.0
259,833 + EX31_RS18425 0.89 +0.1
259,834 - EX31_RS18425 0.89 -0.8
259,834 - EX31_RS18425 0.89 -0.8
259,839 + EX31_RS18425 0.89 -0.2
259,862 + -0.1
259,863 - +0.6
259,863 - +0.4
259,892 + -0.0
259,927 + -0.4
260,049 + -0.1
260,049 + -0.0
260,059 + +0.7
260,063 - -0.5
260,153 + EX31_RS18430 0.20 -1.2
260,172 - EX31_RS18430 0.28 -0.3
260,174 + EX31_RS18430 0.28 +0.1
260,174 + EX31_RS18430 0.28 +0.2
260,174 + EX31_RS18430 0.28 +0.2
260,174 + EX31_RS18430 0.28 -0.0
260,174 + EX31_RS18430 0.28 +0.4
260,175 - EX31_RS18430 0.29 -0.8
260,175 - EX31_RS18430 0.29 +0.6
260,202 + EX31_RS18430 0.39 -1.0
260,202 + EX31_RS18430 0.39 +0.1
260,202 + EX31_RS18430 0.39 +0.1
260,202 + EX31_RS18430 0.39 -1.8
260,202 + EX31_RS18430 0.39 +0.1
260,202 + EX31_RS18430 0.39 +0.1
260,260 + EX31_RS18430 0.62 +0.5
260,260 + EX31_RS18430 0.62 -1.8
260,307 + EX31_RS18430 0.80 -1.8
260,307 + EX31_RS18430 0.80 +0.7
260,308 - EX31_RS18430 0.80 -0.4
260,311 + EX31_RS18430 0.81 -0.2
260,311 + EX31_RS18430 0.81 +0.8
260,311 + EX31_RS18430 0.81 -0.2
260,312 - EX31_RS18430 0.82 +0.6
260,319 + EX31_RS18430 0.84 -0.6
260,367 + -0.6
260,385 - -0.8
260,407 + +0.4
260,407 + -0.4
260,489 + +0.3
260,490 - +0.9
260,490 - -2.4
260,574 - -0.3
260,582 + -0.4
260,583 - +1.1
260,653 - EX31_RS18435 0.31 -0.9
260,698 - EX31_RS18435 0.47 +0.8
260,698 - EX31_RS18435 0.47 -1.2
260,719 + EX31_RS18435 0.55 -0.4
260,719 + EX31_RS18435 0.55 -1.3
260,719 + EX31_RS18435 0.55 -1.7
260,719 + EX31_RS18435 0.55 -0.4
260,720 - EX31_RS18435 0.55 +0.3
260,720 - EX31_RS18435 0.55 -0.1
260,720 - EX31_RS18435 0.55 +0.5
260,720 - EX31_RS18435 0.55 -1.3
260,733 + EX31_RS18435 0.60 -0.6
260,734 - EX31_RS18435 0.60 +0.4
260,734 - EX31_RS18435 0.60 -0.1
260,738 - EX31_RS18435 0.61 -0.6
260,812 - EX31_RS18435 0.88 +0.1
260,850 + -1.6
260,850 + -0.2
260,989 + EX31_RS18440 0.64 -0.0
260,990 - EX31_RS18440 0.64 -0.1
260,990 - EX31_RS18440 0.64 -1.3
260,995 + EX31_RS18440 0.67 -0.8
260,995 + EX31_RS18440 0.67 -2.5
260,996 - EX31_RS18440 0.67 +1.0
261,008 + EX31_RS18440 0.73 +0.2
261,008 + EX31_RS18440 0.73 -1.2
261,068 + +0.6
261,069 - +0.5
261,069 - -0.6
261,069 - -0.4
261,128 - EX31_RS18445 0.25 +0.4
261,174 + EX31_RS18445 0.44 -0.0
261,179 - EX31_RS18445 0.46 -0.9
261,245 - EX31_RS18445 0.73 -0.3
261,370 + -0.0
261,371 - -3.2
261,379 + -0.5
261,402 + +1.4
261,406 - -2.7
261,567 + EX31_RS18450 0.40 -0.0
261,606 - EX31_RS18450 0.47 +0.1
261,654 - EX31_RS18450 0.57 -1.1
261,763 + EX31_RS18450 0.79 +0.2
261,844 - +1.2
261,844 - +0.5

Or see this region's nucleotide sequence