Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS16970 and EX31_RS16975 are separated by 169 nucleotides EX31_RS16975 and EX31_RS16980 are separated by 39 nucleotides EX31_RS16980 and EX31_RS16985 are separated by 323 nucleotides
EX31_RS16970: EX31_RS16970 - MFS transporter, at 3,659 to 5,152
_RS16970
EX31_RS16975: EX31_RS16975 - hypothetical protein, at 5,322 to 5,825
_RS16975
EX31_RS16980: EX31_RS16980 - ATP-dependent dethiobiotin synthetase BioD, at 5,865 to 6,551
_RS16980
EX31_RS16985: EX31_RS16985 - ROK family transcriptional regulator, at 6,875 to 8,098
_RS16985
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4.914 kb on + strand, within EX31_RS16970 at 4.915 kb on - strand, within EX31_RS16970 at 4.915 kb on - strand, within EX31_RS16970 at 4.915 kb on - strand, within EX31_RS16970 at 4.915 kb on - strand, within EX31_RS16970 at 4.936 kb on - strand, within EX31_RS16970 at 4.936 kb on - strand, within EX31_RS16970 at 4.968 kb on + strand, within EX31_RS16970 at 5.054 kb on + strand at 5.097 kb on - strand at 5.097 kb on - strand at 5.129 kb on + strand at 5.129 kb on + strand at 5.209 kb on - strand at 5.266 kb on - strand at 5.281 kb on - strand at 5.283 kb on - strand at 5.283 kb on - strand at 5.283 kb on - strand at 5.323 kb on + strand at 5.355 kb on + strand at 5.379 kb on + strand, within EX31_RS16975 at 5.380 kb on - strand, within EX31_RS16975 at 5.423 kb on + strand, within EX31_RS16975 at 5.456 kb on + strand, within EX31_RS16975 at 5.500 kb on - strand, within EX31_RS16975 at 5.500 kb on - strand, within EX31_RS16975 at 5.519 kb on + strand, within EX31_RS16975 at 5.546 kb on + strand, within EX31_RS16975 at 5.546 kb on + strand, within EX31_RS16975 at 5.546 kb on + strand, within EX31_RS16975 at 5.547 kb on - strand, within EX31_RS16975 at 5.547 kb on - strand, within EX31_RS16975 at 5.613 kb on - strand, within EX31_RS16975 at 5.641 kb on - strand, within EX31_RS16975 at 5.665 kb on - strand, within EX31_RS16975 at 5.666 kb on + strand, within EX31_RS16975 at 5.667 kb on - strand, within EX31_RS16975 at 5.667 kb on - strand, within EX31_RS16975 at 5.680 kb on + strand, within EX31_RS16975 at 5.716 kb on + strand, within EX31_RS16975 at 5.716 kb on + strand, within EX31_RS16975 at 5.716 kb on + strand, within EX31_RS16975 at 5.717 kb on - strand, within EX31_RS16975 at 5.717 kb on - strand, within EX31_RS16975 at 5.717 kb on - strand, within EX31_RS16975 at 5.717 kb on - strand, within EX31_RS16975 at 5.843 kb on - strand at 5.874 kb on - strand at 6.010 kb on + strand, within EX31_RS16980 at 6.029 kb on + strand, within EX31_RS16980 at 6.060 kb on + strand, within EX31_RS16980 at 6.127 kb on - strand, within EX31_RS16980 at 6.127 kb on - strand, within EX31_RS16980 at 6.136 kb on - strand, within EX31_RS16980 at 6.175 kb on - strand, within EX31_RS16980 at 6.222 kb on + strand, within EX31_RS16980 at 6.242 kb on + strand, within EX31_RS16980 at 6.308 kb on + strand, within EX31_RS16980 at 6.308 kb on + strand, within EX31_RS16980 at 6.309 kb on - strand, within EX31_RS16980 at 6.507 kb on - strand at 6.591 kb on + strand at 6.592 kb on - strand at 6.717 kb on + strand at 6.717 kb on + strand at 6.720 kb on - strand at 6.750 kb on + strand at 6.751 kb on - strand at 6.751 kb on - strand at 6.752 kb on + strand at 6.772 kb on - strand at 6.854 kb on + strand at 6.911 kb on - strand at 6.915 kb on - strand at 6.915 kb on - strand at 6.997 kb on - strand at 7.148 kb on - strand, within EX31_RS16985 at 7.230 kb on - strand, within EX31_RS16985 at 7.483 kb on - strand, within EX31_RS16985 at 7.485 kb on - strand, within EX31_RS16985 at 7.485 kb on - strand, within EX31_RS16985 at 7.527 kb on + strand, within EX31_RS16985 at 7.527 kb on + strand, within EX31_RS16985 at 7.527 kb on + strand, within EX31_RS16985 at 7.528 kb on - strand, within EX31_RS16985 at 7.528 kb on - strand, within EX31_RS16985 at 7.528 kb on - strand, within EX31_RS16985 at 7.528 kb on - strand, within EX31_RS16985 at 7.528 kb on - strand, within EX31_RS16985
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 4,914 + EX31_RS16970 0.84 +2.0 4,915 - EX31_RS16970 0.84 -0.5 4,915 - EX31_RS16970 0.84 -1.1 4,915 - EX31_RS16970 0.84 +0.9 4,915 - EX31_RS16970 0.84 -0.6 4,936 - EX31_RS16970 0.85 -1.3 4,936 - EX31_RS16970 0.85 +0.1 4,968 + EX31_RS16970 0.88 +0.7 5,054 + -0.2 5,097 - +1.5 5,097 - +0.3 5,129 + +0.1 5,129 + +0.8 5,209 - -0.0 5,266 - -0.8 5,281 - -0.3 5,283 - -0.8 5,283 - +0.9 5,283 - +0.3 5,323 + -0.9 5,355 + -1.7 5,379 + EX31_RS16975 0.11 -2.5 5,380 - EX31_RS16975 0.12 -0.1 5,423 + EX31_RS16975 0.20 +2.7 5,456 + EX31_RS16975 0.27 -0.4 5,500 - EX31_RS16975 0.35 +1.3 5,500 - EX31_RS16975 0.35 -0.8 5,519 + EX31_RS16975 0.39 +1.6 5,546 + EX31_RS16975 0.44 -0.6 5,546 + EX31_RS16975 0.44 -2.0 5,546 + EX31_RS16975 0.44 -1.4 5,547 - EX31_RS16975 0.45 -2.6 5,547 - EX31_RS16975 0.45 +0.8 5,613 - EX31_RS16975 0.58 +1.7 5,641 - EX31_RS16975 0.63 +0.6 5,665 - EX31_RS16975 0.68 -1.2 5,666 + EX31_RS16975 0.68 +0.0 5,667 - EX31_RS16975 0.68 -1.1 5,667 - EX31_RS16975 0.68 -0.2 5,680 + EX31_RS16975 0.71 -1.3 5,716 + EX31_RS16975 0.78 -1.2 5,716 + EX31_RS16975 0.78 -1.8 5,716 + EX31_RS16975 0.78 +1.2 5,717 - EX31_RS16975 0.78 +0.7 5,717 - EX31_RS16975 0.78 -0.3 5,717 - EX31_RS16975 0.78 +0.6 5,717 - EX31_RS16975 0.78 -1.6 5,843 - +0.6 5,874 - -1.1 6,010 + EX31_RS16980 0.21 -2.3 6,029 + EX31_RS16980 0.24 -0.1 6,060 + EX31_RS16980 0.28 -1.0 6,127 - EX31_RS16980 0.38 +1.1 6,127 - EX31_RS16980 0.38 -1.8 6,136 - EX31_RS16980 0.39 -0.2 6,175 - EX31_RS16980 0.45 -0.1 6,222 + EX31_RS16980 0.52 +0.2 6,242 + EX31_RS16980 0.55 -2.0 6,308 + EX31_RS16980 0.64 -2.5 6,308 + EX31_RS16980 0.64 -0.3 6,309 - EX31_RS16980 0.65 +0.2 6,507 - -1.5 6,591 + -1.1 6,592 - -0.2 6,717 + -1.0 6,717 + +1.0 6,720 - -1.8 6,750 + +0.1 6,751 - -0.5 6,751 - +0.5 6,752 + -2.1 6,772 - -0.8 6,854 + +0.5 6,911 - -1.9 6,915 - +0.9 6,915 - -0.3 6,997 - +0.7 7,148 - EX31_RS16985 0.22 -1.5 7,230 - EX31_RS16985 0.29 -1.7 7,483 - EX31_RS16985 0.50 -0.5 7,485 - EX31_RS16985 0.50 -0.0 7,485 - EX31_RS16985 0.50 +0.0 7,527 + EX31_RS16985 0.53 -0.6 7,527 + EX31_RS16985 0.53 +0.7 7,527 + EX31_RS16985 0.53 -0.9 7,528 - EX31_RS16985 0.53 -0.0 7,528 - EX31_RS16985 0.53 -0.9 7,528 - EX31_RS16985 0.53 +0.8 7,528 - EX31_RS16985 0.53 +0.1 7,528 - EX31_RS16985 0.53 -1.6
Or see this region's nucleotide sequence