Strain Fitness in Rahnella sp. WP5 around EX31_RS11500

Experiment: NF_with10percent_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS11495 and EX31_RS11500 are separated by 50 nucleotidesEX31_RS11500 and EX31_RS11505 are separated by 123 nucleotidesEX31_RS11505 and EX31_RS11510 overlap by 4 nucleotides EX31_RS11495: EX31_RS11495 - glycosyltransferase family 2 protein, at 236,205 to 237,101 _RS11495 EX31_RS11500: EX31_RS11500 - glycosyltransferase family 2 protein, at 237,152 to 237,985 _RS11500 EX31_RS11505: EX31_RS11505 - dTDP-4-dehydrorhamnose reductase, at 238,109 to 238,981 _RS11505 EX31_RS11510: EX31_RS11510 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 238,978 to 239,514 _RS11510 Position (kb) 237 238Strain fitness (log2 ratio) -2 -1 0 1at 236.197 kb on - strandat 237.372 kb on - strand, within EX31_RS11500at 237.391 kb on - strand, within EX31_RS11500at 237.513 kb on + strand, within EX31_RS11500at 237.727 kb on - strand, within EX31_RS11500at 237.807 kb on - strand, within EX31_RS11500at 237.925 kb on - strandat 237.928 kb on + strandat 237.929 kb on - strandat 238.067 kb on - strandat 238.096 kb on - strandat 238.111 kb on - strandat 238.256 kb on - strand, within EX31_RS11505at 238.369 kb on - strand, within EX31_RS11505at 238.904 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with10percent_fullN
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236,197 - +1.0
237,372 - EX31_RS11500 0.26 -1.2
237,391 - EX31_RS11500 0.29 -0.8
237,513 + EX31_RS11500 0.43 -1.2
237,727 - EX31_RS11500 0.69 -0.7
237,807 - EX31_RS11500 0.79 +0.5
237,925 - -0.5
237,928 + -0.6
237,929 - -0.7
238,067 - +0.2
238,096 - -0.0
238,111 - +0.5
238,256 - EX31_RS11505 0.17 -0.5
238,369 - EX31_RS11505 0.30 -0.1
238,904 + -2.7

Or see this region's nucleotide sequence