Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10585 and EX31_RS10590 are separated by 3 nucleotides EX31_RS10590 and EX31_RS10595 are separated by 347 nucleotides EX31_RS10595 and EX31_RS10600 are separated by 97 nucleotides
EX31_RS10585: EX31_RS10585 - septum site-determining protein MinD, at 29,207 to 30,019
_RS10585
EX31_RS10590: EX31_RS10590 - cell division topological specificity factor MinE, at 30,023 to 30,292
_RS10590
EX31_RS10595: EX31_RS10595 - ribonuclease D, at 30,640 to 31,761
_RS10595
EX31_RS10600: EX31_RS10600 - long-chain-fatty-acid--CoA ligase FadD, at 31,859 to 33,556
_RS10600
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.753 kb on + strand, within EX31_RS10585 at 29.921 kb on + strand, within EX31_RS10585 at 29.921 kb on + strand, within EX31_RS10585 at 29.929 kb on + strand, within EX31_RS10585 at 30.243 kb on + strand, within EX31_RS10590 at 30.370 kb on + strand at 30.388 kb on + strand at 30.420 kb on + strand at 30.511 kb on - strand at 30.511 kb on - strand at 31.290 kb on - strand, within EX31_RS10595 at 31.310 kb on + strand, within EX31_RS10595 at 31.311 kb on - strand, within EX31_RS10595 at 31.311 kb on - strand, within EX31_RS10595 at 31.625 kb on - strand, within EX31_RS10595 at 31.630 kb on + strand, within EX31_RS10595 at 31.630 kb on + strand, within EX31_RS10595 at 31.631 kb on - strand, within EX31_RS10595 at 31.631 kb on - strand, within EX31_RS10595 at 31.631 kb on - strand, within EX31_RS10595 at 31.631 kb on - strand, within EX31_RS10595 at 31.652 kb on + strand at 31.653 kb on - strand at 31.783 kb on - strand at 31.791 kb on - strand at 31.793 kb on - strand at 32.118 kb on - strand, within EX31_RS10600 at 32.161 kb on - strand, within EX31_RS10600 at 32.214 kb on - strand, within EX31_RS10600 at 32.214 kb on - strand, within EX31_RS10600 at 32.237 kb on - strand, within EX31_RS10600 at 32.271 kb on - strand, within EX31_RS10600 at 32.315 kb on - strand, within EX31_RS10600 at 32.431 kb on - strand, within EX31_RS10600 at 32.431 kb on - strand, within EX31_RS10600 at 32.431 kb on - strand, within EX31_RS10600 at 32.635 kb on + strand, within EX31_RS10600 at 32.635 kb on + strand, within EX31_RS10600 at 32.636 kb on - strand, within EX31_RS10600 at 32.636 kb on - strand, within EX31_RS10600 at 32.665 kb on - strand, within EX31_RS10600
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 29,753 + EX31_RS10585 0.67 -0.2 29,921 + EX31_RS10585 0.88 +0.9 29,921 + EX31_RS10585 0.88 +0.1 29,929 + EX31_RS10585 0.89 +0.2 30,243 + EX31_RS10590 0.81 -1.9 30,370 + -0.2 30,388 + +0.5 30,420 + +0.0 30,511 - -1.7 30,511 - -0.3 31,290 - EX31_RS10595 0.58 -1.1 31,310 + EX31_RS10595 0.60 -1.3 31,311 - EX31_RS10595 0.60 -1.6 31,311 - EX31_RS10595 0.60 +0.0 31,625 - EX31_RS10595 0.88 +0.6 31,630 + EX31_RS10595 0.88 -1.1 31,630 + EX31_RS10595 0.88 -1.1 31,631 - EX31_RS10595 0.88 -0.6 31,631 - EX31_RS10595 0.88 -0.7 31,631 - EX31_RS10595 0.88 -0.9 31,631 - EX31_RS10595 0.88 +0.3 31,652 + -0.3 31,653 - -1.0 31,783 - +0.9 31,791 - -0.7 31,793 - +0.4 32,118 - EX31_RS10600 0.15 -0.6 32,161 - EX31_RS10600 0.18 +0.5 32,214 - EX31_RS10600 0.21 +0.3 32,214 - EX31_RS10600 0.21 +0.2 32,237 - EX31_RS10600 0.22 +0.3 32,271 - EX31_RS10600 0.24 +1.0 32,315 - EX31_RS10600 0.27 +0.1 32,431 - EX31_RS10600 0.34 +0.7 32,431 - EX31_RS10600 0.34 -2.0 32,431 - EX31_RS10600 0.34 -0.9 32,635 + EX31_RS10600 0.46 -0.1 32,635 + EX31_RS10600 0.46 -1.1 32,636 - EX31_RS10600 0.46 -0.7 32,636 - EX31_RS10600 0.46 -0.4 32,665 - EX31_RS10600 0.47 -0.1
Or see this region's nucleotide sequence