Strain Fitness in Rahnella sp. WP5 around EX31_RS10595

Experiment: NF_with10percent_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS10585 and EX31_RS10590 are separated by 3 nucleotidesEX31_RS10590 and EX31_RS10595 are separated by 347 nucleotidesEX31_RS10595 and EX31_RS10600 are separated by 97 nucleotides EX31_RS10585: EX31_RS10585 - septum site-determining protein MinD, at 29,207 to 30,019 _RS10585 EX31_RS10590: EX31_RS10590 - cell division topological specificity factor MinE, at 30,023 to 30,292 _RS10590 EX31_RS10595: EX31_RS10595 - ribonuclease D, at 30,640 to 31,761 _RS10595 EX31_RS10600: EX31_RS10600 - long-chain-fatty-acid--CoA ligase FadD, at 31,859 to 33,556 _RS10600 Position (kb) 30 31 32Strain fitness (log2 ratio) -2 -1 0 1at 29.753 kb on + strand, within EX31_RS10585at 29.921 kb on + strand, within EX31_RS10585at 29.921 kb on + strand, within EX31_RS10585at 29.929 kb on + strand, within EX31_RS10585at 30.243 kb on + strand, within EX31_RS10590at 30.370 kb on + strandat 30.388 kb on + strandat 30.420 kb on + strandat 30.511 kb on - strandat 30.511 kb on - strandat 31.290 kb on - strand, within EX31_RS10595at 31.310 kb on + strand, within EX31_RS10595at 31.311 kb on - strand, within EX31_RS10595at 31.311 kb on - strand, within EX31_RS10595at 31.625 kb on - strand, within EX31_RS10595at 31.630 kb on + strand, within EX31_RS10595at 31.630 kb on + strand, within EX31_RS10595at 31.631 kb on - strand, within EX31_RS10595at 31.631 kb on - strand, within EX31_RS10595at 31.631 kb on - strand, within EX31_RS10595at 31.631 kb on - strand, within EX31_RS10595at 31.652 kb on + strandat 31.653 kb on - strandat 31.783 kb on - strandat 31.791 kb on - strandat 31.793 kb on - strandat 32.118 kb on - strand, within EX31_RS10600at 32.161 kb on - strand, within EX31_RS10600at 32.214 kb on - strand, within EX31_RS10600at 32.214 kb on - strand, within EX31_RS10600at 32.237 kb on - strand, within EX31_RS10600at 32.271 kb on - strand, within EX31_RS10600at 32.315 kb on - strand, within EX31_RS10600at 32.431 kb on - strand, within EX31_RS10600at 32.431 kb on - strand, within EX31_RS10600at 32.431 kb on - strand, within EX31_RS10600at 32.635 kb on + strand, within EX31_RS10600at 32.635 kb on + strand, within EX31_RS10600at 32.636 kb on - strand, within EX31_RS10600at 32.636 kb on - strand, within EX31_RS10600at 32.665 kb on - strand, within EX31_RS10600

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with10percent_fullN
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29,753 + EX31_RS10585 0.67 -0.2
29,921 + EX31_RS10585 0.88 +0.9
29,921 + EX31_RS10585 0.88 +0.1
29,929 + EX31_RS10585 0.89 +0.2
30,243 + EX31_RS10590 0.81 -1.9
30,370 + -0.2
30,388 + +0.5
30,420 + +0.0
30,511 - -1.7
30,511 - -0.3
31,290 - EX31_RS10595 0.58 -1.1
31,310 + EX31_RS10595 0.60 -1.3
31,311 - EX31_RS10595 0.60 -1.6
31,311 - EX31_RS10595 0.60 +0.0
31,625 - EX31_RS10595 0.88 +0.6
31,630 + EX31_RS10595 0.88 -1.1
31,630 + EX31_RS10595 0.88 -1.1
31,631 - EX31_RS10595 0.88 -0.6
31,631 - EX31_RS10595 0.88 -0.7
31,631 - EX31_RS10595 0.88 -0.9
31,631 - EX31_RS10595 0.88 +0.3
31,652 + -0.3
31,653 - -1.0
31,783 - +0.9
31,791 - -0.7
31,793 - +0.4
32,118 - EX31_RS10600 0.15 -0.6
32,161 - EX31_RS10600 0.18 +0.5
32,214 - EX31_RS10600 0.21 +0.3
32,214 - EX31_RS10600 0.21 +0.2
32,237 - EX31_RS10600 0.22 +0.3
32,271 - EX31_RS10600 0.24 +1.0
32,315 - EX31_RS10600 0.27 +0.1
32,431 - EX31_RS10600 0.34 +0.7
32,431 - EX31_RS10600 0.34 -2.0
32,431 - EX31_RS10600 0.34 -0.9
32,635 + EX31_RS10600 0.46 -0.1
32,635 + EX31_RS10600 0.46 -1.1
32,636 - EX31_RS10600 0.46 -0.7
32,636 - EX31_RS10600 0.46 -0.4
32,665 - EX31_RS10600 0.47 -0.1

Or see this region's nucleotide sequence