Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS15525 and EX31_RS15530 are separated by 140 nucleotides EX31_RS15530 and EX31_RS15535 overlap by 4 nucleotides EX31_RS15535 and EX31_RS15540 are separated by 133 nucleotides
EX31_RS15525: EX31_RS15525 - nitrogen assimilation transcriptional regulator, at 2,027 to 2,950
_RS15525
EX31_RS15530: EX31_RS15530 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 3,091 to 3,531
_RS15530
EX31_RS15535: EX31_RS15535 - LamB/YcsF family protein, at 3,528 to 4,310
_RS15535
EX31_RS15540: EX31_RS15540 - SDR family oxidoreductase, at 4,444 to 5,193
_RS15540
Position (kb)
3
4 Strain fitness (log2 ratio)
-2
-1
0
1 at 2.220 kb on - strand, within EX31_RS15525 at 2.501 kb on - strand, within EX31_RS15525 at 2.501 kb on - strand, within EX31_RS15525 at 2.591 kb on + strand, within EX31_RS15525 at 2.591 kb on + strand, within EX31_RS15525 at 2.591 kb on + strand, within EX31_RS15525 at 2.592 kb on - strand, within EX31_RS15525 at 2.592 kb on - strand, within EX31_RS15525 at 2.839 kb on + strand, within EX31_RS15525 at 2.948 kb on + strand at 3.011 kb on - strand at 3.011 kb on - strand at 3.011 kb on - strand at 3.011 kb on - strand at 3.022 kb on - strand at 3.205 kb on + strand, within EX31_RS15530 at 3.320 kb on + strand, within EX31_RS15530 at 3.534 kb on + strand at 3.534 kb on + strand at 3.576 kb on + strand at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.700 kb on + strand, within EX31_RS15535 at 3.786 kb on + strand, within EX31_RS15535 at 3.786 kb on + strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.787 kb on - strand, within EX31_RS15535 at 3.796 kb on - strand, within EX31_RS15535 at 3.879 kb on - strand, within EX31_RS15535 at 4.232 kb on + strand at 4.232 kb on + strand at 4.232 kb on + strand at 4.232 kb on + strand at 4.233 kb on - strand at 4.233 kb on - strand at 4.309 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.322 kb on - strand at 4.323 kb on + strand at 4.323 kb on + strand at 4.323 kb on + strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.324 kb on - strand at 4.351 kb on + strand at 4.351 kb on + strand at 4.351 kb on + strand at 4.352 kb on - strand at 4.352 kb on - strand at 4.359 kb on - strand at 4.359 kb on - strand at 4.361 kb on - strand at 4.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 2,220 - EX31_RS15525 0.21 -2.1 2,501 - EX31_RS15525 0.51 -1.1 2,501 - EX31_RS15525 0.51 +0.9 2,591 + EX31_RS15525 0.61 -0.2 2,591 + EX31_RS15525 0.61 +1.5 2,591 + EX31_RS15525 0.61 -0.0 2,592 - EX31_RS15525 0.61 -0.8 2,592 - EX31_RS15525 0.61 -0.4 2,839 + EX31_RS15525 0.88 +0.5 2,948 + -1.9 3,011 - +1.4 3,011 - -1.4 3,011 - -1.7 3,011 - -0.5 3,022 - -0.4 3,205 + EX31_RS15530 0.26 -0.6 3,320 + EX31_RS15530 0.52 +0.9 3,534 + -0.2 3,534 + -0.1 3,576 + +0.3 3,700 + EX31_RS15535 0.22 -0.1 3,700 + EX31_RS15535 0.22 -0.4 3,700 + EX31_RS15535 0.22 -0.3 3,700 + EX31_RS15535 0.22 -0.6 3,700 + EX31_RS15535 0.22 -0.1 3,700 + EX31_RS15535 0.22 +0.2 3,786 + EX31_RS15535 0.33 -1.2 3,786 + EX31_RS15535 0.33 -2.4 3,787 - EX31_RS15535 0.33 -0.2 3,787 - EX31_RS15535 0.33 -0.6 3,787 - EX31_RS15535 0.33 -0.4 3,796 - EX31_RS15535 0.34 -0.9 3,879 - EX31_RS15535 0.45 -1.8 4,232 + -0.6 4,232 + -1.1 4,232 + +1.4 4,232 + -0.5 4,233 - +0.4 4,233 - +1.4 4,309 - -0.5 4,322 - +0.4 4,322 - +0.9 4,322 - +0.4 4,323 + +1.7 4,323 + -0.3 4,323 + -1.1 4,324 - -0.2 4,324 - -0.9 4,324 - +0.1 4,324 - +0.9 4,324 - -1.7 4,351 + -0.4 4,351 + -0.0 4,351 + +0.1 4,352 - -2.1 4,352 - +0.6 4,359 - +0.1 4,359 - +0.2 4,361 - +0.1 4,361 - +0.1
Or see this region's nucleotide sequence