Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10425 and EX31_RS10430 are separated by 3 nucleotides EX31_RS10430 and EX31_RS10435 overlap by 4 nucleotides EX31_RS10435 and EX31_RS10440 are separated by 116 nucleotides
EX31_RS10425: EX31_RS10425 - transporter substrate-binding domain-containing protein, at 296,134 to 296,973
_RS10425
EX31_RS10430: EX31_RS10430 - 5-oxoprolinase subunit PxpB, at 296,977 to 297,687
_RS10430
EX31_RS10435: EX31_RS10435 - biotin-dependent carboxyltransferase family protein, at 297,684 to 298,685
_RS10435
EX31_RS10440: EX31_RS10440 - IS110 family transposase, at 298,802 to 299,063
_RS10440
Position (kb) 297
298
299 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 296.872 kb on - strand, within EX31_RS10425 at 296.933 kb on + strand at 296.933 kb on + strand at 296.934 kb on - strand at 296.934 kb on - strand at 296.934 kb on - strand at 296.934 kb on - strand at 296.934 kb on - strand at 297.018 kb on + strand at 297.406 kb on + strand, within EX31_RS10430 at 297.406 kb on + strand, within EX31_RS10430 at 297.406 kb on + strand, within EX31_RS10430 at 297.446 kb on - strand, within EX31_RS10430 at 297.446 kb on - strand, within EX31_RS10430 at 297.504 kb on + strand, within EX31_RS10430 at 297.504 kb on + strand, within EX31_RS10430 at 297.505 kb on - strand, within EX31_RS10430 at 297.505 kb on - strand, within EX31_RS10430 at 297.505 kb on - strand, within EX31_RS10430 at 297.731 kb on + strand at 298.017 kb on + strand, within EX31_RS10435 at 298.033 kb on - strand, within EX31_RS10435 at 298.114 kb on + strand, within EX31_RS10435 at 298.228 kb on + strand, within EX31_RS10435 at 298.268 kb on + strand, within EX31_RS10435 at 298.534 kb on + strand, within EX31_RS10435 at 298.535 kb on - strand, within EX31_RS10435 at 298.655 kb on + strand at 298.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 296,872 - EX31_RS10425 0.88 -2.6 296,933 + +0.1 296,933 + -1.4 296,934 - +0.7 296,934 - -2.2 296,934 - -0.0 296,934 - -0.2 296,934 - +0.1 297,018 + -0.2 297,406 + EX31_RS10430 0.60 -0.2 297,406 + EX31_RS10430 0.60 +2.8 297,406 + EX31_RS10430 0.60 +0.6 297,446 - EX31_RS10430 0.66 -0.3 297,446 - EX31_RS10430 0.66 +1.1 297,504 + EX31_RS10430 0.74 -2.2 297,504 + EX31_RS10430 0.74 -0.0 297,505 - EX31_RS10430 0.74 +0.6 297,505 - EX31_RS10430 0.74 -0.6 297,505 - EX31_RS10430 0.74 -0.2 297,731 + +0.2 298,017 + EX31_RS10435 0.33 -0.4 298,033 - EX31_RS10435 0.35 +0.4 298,114 + EX31_RS10435 0.43 -2.3 298,228 + EX31_RS10435 0.54 -2.1 298,268 + EX31_RS10435 0.58 -1.0 298,534 + EX31_RS10435 0.85 -1.0 298,535 - EX31_RS10435 0.85 -1.3 298,655 + +1.5 298,732 - -1.2
Or see this region's nucleotide sequence