Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23460 and EX31_RS23465 are separated by 14 nucleotides EX31_RS23465 and EX31_RS25650 are separated by 10 nucleotides EX31_RS25650 and EX31_RS23470 are separated by 2 nucleotides EX31_RS23470 and EX31_RS23475 are separated by 149 nucleotides EX31_RS23475 and EX31_RS23480 overlap by 4 nucleotides
EX31_RS23460: EX31_RS23460 - cytochrome ubiquinol oxidase subunit I, at 461,314 to 462,882
_RS23460
EX31_RS23465: EX31_RS23465 - cytochrome d ubiquinol oxidase subunit II, at 462,897 to 464,036
_RS23465
EX31_RS25650: EX31_RS25650 - cytochrome bd-I oxidase subunit CydX, at 464,047 to 464,160
_RS25650
EX31_RS23470: EX31_RS23470 - cyd operon protein YbgE, at 464,163 to 464,456
_RS23470
EX31_RS23475: EX31_RS23475 - tol-pal system-associated acyl-CoA thioesterase, at 464,606 to 465,010
_RS23475
EX31_RS23480: EX31_RS23480 - Tol-Pal system protein TolQ, at 465,007 to 465,696
_RS23480
Position (kb) 462
463
464
465 Strain fitness (log2 ratio) -2
-1
0
1
2 at 461.917 kb on - strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.926 kb on + strand, within EX31_RS23460 at 461.929 kb on + strand, within EX31_RS23460 at 461.929 kb on + strand, within EX31_RS23460 at 461.930 kb on - strand, within EX31_RS23460 at 461.936 kb on + strand, within EX31_RS23460 at 462.056 kb on + strand, within EX31_RS23460 at 462.164 kb on - strand, within EX31_RS23460 at 462.164 kb on - strand, within EX31_RS23460 at 462.181 kb on + strand, within EX31_RS23460 at 462.217 kb on - strand, within EX31_RS23460 at 462.287 kb on - strand, within EX31_RS23460 at 462.287 kb on - strand, within EX31_RS23460 at 462.318 kb on + strand, within EX31_RS23460 at 462.371 kb on - strand, within EX31_RS23460 at 462.375 kb on + strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.376 kb on - strand, within EX31_RS23460 at 462.472 kb on + strand, within EX31_RS23460 at 462.473 kb on - strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.538 kb on + strand, within EX31_RS23460 at 462.541 kb on - strand, within EX31_RS23460 at 462.578 kb on + strand, within EX31_RS23460 at 462.579 kb on - strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.725 kb on + strand, within EX31_RS23460 at 462.757 kb on + strand at 462.799 kb on + strand at 462.800 kb on - strand at 462.839 kb on - strand at 462.883 kb on + strand at 462.883 kb on + strand at 462.884 kb on - strand at 462.896 kb on + strand at 462.896 kb on + strand at 462.896 kb on + strand at 462.897 kb on - strand at 462.897 kb on - strand at 462.906 kb on + strand at 462.991 kb on + strand at 463.091 kb on + strand, within EX31_RS23465 at 463.132 kb on - strand, within EX31_RS23465 at 463.155 kb on + strand, within EX31_RS23465 at 463.155 kb on + strand, within EX31_RS23465 at 463.156 kb on - strand, within EX31_RS23465 at 463.430 kb on + strand, within EX31_RS23465 at 463.451 kb on + strand, within EX31_RS23465 at 463.451 kb on + strand, within EX31_RS23465 at 463.469 kb on + strand, within EX31_RS23465 at 463.470 kb on - strand, within EX31_RS23465 at 463.708 kb on + strand, within EX31_RS23465 at 463.754 kb on + strand, within EX31_RS23465 at 463.754 kb on + strand, within EX31_RS23465 at 463.778 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.823 kb on + strand, within EX31_RS23465 at 463.824 kb on - strand, within EX31_RS23465 at 463.824 kb on - strand, within EX31_RS23465 at 463.847 kb on + strand, within EX31_RS23465 at 463.848 kb on - strand, within EX31_RS23465 at 463.848 kb on - strand, within EX31_RS23465 at 463.892 kb on + strand, within EX31_RS23465 at 463.892 kb on + strand, within EX31_RS23465 at 463.892 kb on + strand, within EX31_RS23465 at 463.892 kb on + strand, within EX31_RS23465 at 463.892 kb on + strand, within EX31_RS23465 at 463.893 kb on - strand, within EX31_RS23465 at 463.893 kb on - strand, within EX31_RS23465 at 463.931 kb on + strand at 463.931 kb on + strand at 463.932 kb on - strand at 463.932 kb on - strand at 463.932 kb on - strand at 463.957 kb on - strand at 463.957 kb on - strand at 463.971 kb on + strand at 463.974 kb on + strand at 463.974 kb on + strand at 463.975 kb on - strand at 463.975 kb on - strand at 464.004 kb on + strand at 464.004 kb on + strand at 464.004 kb on + strand at 464.004 kb on + strand at 464.005 kb on - strand at 464.005 kb on - strand at 464.031 kb on + strand at 464.031 kb on + strand at 464.031 kb on + strand at 464.032 kb on - strand at 464.032 kb on - strand at 464.032 kb on - strand at 464.034 kb on + strand at 464.158 kb on + strand at 464.158 kb on + strand at 464.158 kb on + strand at 464.158 kb on + strand at 464.158 kb on + strand at 464.158 kb on + strand at 464.159 kb on - strand at 464.159 kb on - strand at 464.159 kb on - strand at 464.159 kb on - strand at 464.161 kb on + strand at 464.161 kb on + strand at 464.161 kb on + strand at 464.161 kb on + strand at 464.162 kb on - strand at 464.187 kb on + strand at 464.187 kb on + strand at 464.188 kb on - strand at 464.242 kb on + strand, within EX31_RS23470 at 464.407 kb on + strand, within EX31_RS23470 at 464.426 kb on - strand, within EX31_RS23470 at 464.439 kb on + strand at 464.439 kb on + strand at 464.440 kb on - strand at 464.440 kb on - strand at 464.440 kb on - strand at 464.440 kb on - strand at 464.521 kb on - strand at 464.521 kb on - strand at 464.521 kb on - strand at 464.569 kb on + strand at 464.569 kb on + strand at 464.569 kb on + strand at 464.569 kb on + strand at 464.569 kb on + strand at 464.569 kb on + strand at 464.571 kb on + strand at 464.571 kb on + strand at 464.575 kb on - strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.602 kb on + strand at 464.603 kb on - strand at 464.611 kb on + strand at 464.642 kb on + strand at 464.675 kb on + strand, within EX31_RS23475 at 464.675 kb on + strand, within EX31_RS23475 at 464.699 kb on + strand, within EX31_RS23475 at 464.801 kb on + strand, within EX31_RS23475 at 464.801 kb on + strand, within EX31_RS23475 at 464.820 kb on + strand, within EX31_RS23475 at 464.820 kb on + strand, within EX31_RS23475 at 464.851 kb on + strand, within EX31_RS23475 at 464.851 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.875 kb on + strand, within EX31_RS23475 at 464.980 kb on + strand at 464.980 kb on + strand at 464.980 kb on + strand at 464.980 kb on + strand at 464.981 kb on - strand at 464.986 kb on + strand at 464.986 kb on + strand at 464.986 kb on + strand at 464.986 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand at 465.001 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 461,917 - EX31_RS23460 0.38 -1.0 461,926 + EX31_RS23460 0.39 +0.2 461,926 + EX31_RS23460 0.39 +1.1 461,926 + EX31_RS23460 0.39 +0.1 461,926 + EX31_RS23460 0.39 -1.6 461,926 + EX31_RS23460 0.39 -0.4 461,929 + EX31_RS23460 0.39 -1.3 461,929 + EX31_RS23460 0.39 -1.2 461,930 - EX31_RS23460 0.39 -0.3 461,936 + EX31_RS23460 0.40 +1.0 462,056 + EX31_RS23460 0.47 +0.3 462,164 - EX31_RS23460 0.54 -0.0 462,164 - EX31_RS23460 0.54 -0.7 462,181 + EX31_RS23460 0.55 -0.1 462,217 - EX31_RS23460 0.58 -0.3 462,287 - EX31_RS23460 0.62 -2.0 462,287 - EX31_RS23460 0.62 +0.4 462,318 + EX31_RS23460 0.64 -1.0 462,371 - EX31_RS23460 0.67 +0.5 462,375 + EX31_RS23460 0.68 +0.2 462,376 - EX31_RS23460 0.68 -0.7 462,376 - EX31_RS23460 0.68 -0.6 462,376 - EX31_RS23460 0.68 +0.3 462,472 + EX31_RS23460 0.74 -2.5 462,473 - EX31_RS23460 0.74 -1.8 462,538 + EX31_RS23460 0.78 -2.7 462,538 + EX31_RS23460 0.78 -0.7 462,538 + EX31_RS23460 0.78 -2.6 462,538 + EX31_RS23460 0.78 -0.2 462,538 + EX31_RS23460 0.78 -1.4 462,538 + EX31_RS23460 0.78 -0.6 462,538 + EX31_RS23460 0.78 -0.1 462,541 - EX31_RS23460 0.78 +0.0 462,578 + EX31_RS23460 0.81 -1.3 462,579 - EX31_RS23460 0.81 +0.7 462,725 + EX31_RS23460 0.90 +1.7 462,725 + EX31_RS23460 0.90 -0.9 462,725 + EX31_RS23460 0.90 -0.7 462,725 + EX31_RS23460 0.90 +0.2 462,725 + EX31_RS23460 0.90 +0.1 462,725 + EX31_RS23460 0.90 -2.2 462,725 + EX31_RS23460 0.90 +1.2 462,725 + EX31_RS23460 0.90 +1.3 462,725 + EX31_RS23460 0.90 -0.8 462,757 + -1.9 462,799 + -0.9 462,800 - +0.9 462,839 - +0.5 462,883 + -0.5 462,883 + +0.8 462,884 - -1.0 462,896 + -1.3 462,896 + -0.5 462,896 + -0.5 462,897 - -0.1 462,897 - -1.0 462,906 + -0.9 462,991 + +0.8 463,091 + EX31_RS23465 0.17 -0.3 463,132 - EX31_RS23465 0.21 -1.3 463,155 + EX31_RS23465 0.23 -0.8 463,155 + EX31_RS23465 0.23 +0.4 463,156 - EX31_RS23465 0.23 +0.0 463,430 + EX31_RS23465 0.47 +0.8 463,451 + EX31_RS23465 0.49 +0.3 463,451 + EX31_RS23465 0.49 -0.6 463,469 + EX31_RS23465 0.50 -1.7 463,470 - EX31_RS23465 0.50 +0.2 463,708 + EX31_RS23465 0.71 +0.3 463,754 + EX31_RS23465 0.75 -1.5 463,754 + EX31_RS23465 0.75 -1.1 463,778 + EX31_RS23465 0.77 -0.2 463,823 + EX31_RS23465 0.81 +0.1 463,823 + EX31_RS23465 0.81 +1.0 463,823 + EX31_RS23465 0.81 +1.0 463,823 + EX31_RS23465 0.81 +0.7 463,823 + EX31_RS23465 0.81 +0.0 463,824 - EX31_RS23465 0.81 +0.0 463,824 - EX31_RS23465 0.81 -1.1 463,847 + EX31_RS23465 0.83 -0.0 463,848 - EX31_RS23465 0.83 -0.7 463,848 - EX31_RS23465 0.83 -0.4 463,892 + EX31_RS23465 0.87 -0.6 463,892 + EX31_RS23465 0.87 +0.3 463,892 + EX31_RS23465 0.87 -1.0 463,892 + EX31_RS23465 0.87 -1.2 463,892 + EX31_RS23465 0.87 -1.0 463,893 - EX31_RS23465 0.87 +0.0 463,893 - EX31_RS23465 0.87 -1.4 463,931 + -1.7 463,931 + -1.3 463,932 - +1.7 463,932 - -0.9 463,932 - -0.9 463,957 - +0.0 463,957 - +0.3 463,971 + +1.2 463,974 + +1.5 463,974 + +0.7 463,975 - -0.4 463,975 - +0.2 464,004 + -0.4 464,004 + +0.4 464,004 + -0.4 464,004 + -0.2 464,005 - -1.0 464,005 - +0.4 464,031 + +0.6 464,031 + -0.5 464,031 + -0.5 464,032 - -0.2 464,032 - +1.0 464,032 - +1.4 464,034 + +1.0 464,158 + +0.2 464,158 + +0.9 464,158 + +0.3 464,158 + -0.5 464,158 + -0.3 464,158 + -0.6 464,159 - -0.2 464,159 - -2.2 464,159 - -1.4 464,159 - +0.0 464,161 + +0.1 464,161 + -1.0 464,161 + -1.2 464,161 + -0.5 464,162 - -2.2 464,187 + -0.1 464,187 + +0.6 464,188 - -1.1 464,242 + EX31_RS23470 0.27 -2.3 464,407 + EX31_RS23470 0.83 +1.0 464,426 - EX31_RS23470 0.89 -0.4 464,439 + -0.4 464,439 + +0.2 464,440 - -0.3 464,440 - +0.8 464,440 - +1.3 464,440 - -0.4 464,521 - +0.7 464,521 - -0.1 464,521 - +0.6 464,569 + -1.2 464,569 + -0.6 464,569 + -0.9 464,569 + -0.7 464,569 + +0.1 464,569 + -1.5 464,571 + -0.9 464,571 + -2.0 464,575 - +0.7 464,602 + -0.4 464,602 + +0.6 464,602 + -1.0 464,602 + -0.2 464,602 + +0.8 464,602 + +0.2 464,602 + -0.5 464,602 + +0.1 464,602 + +0.2 464,602 + +1.9 464,602 + +0.5 464,602 + -0.8 464,602 + -0.9 464,602 + +0.0 464,602 + +0.4 464,602 + -0.6 464,603 - -1.2 464,611 + +0.4 464,642 + +1.2 464,675 + EX31_RS23475 0.17 +0.1 464,675 + EX31_RS23475 0.17 -0.2 464,699 + EX31_RS23475 0.23 +0.4 464,801 + EX31_RS23475 0.48 -2.0 464,801 + EX31_RS23475 0.48 -0.1 464,820 + EX31_RS23475 0.53 -0.4 464,820 + EX31_RS23475 0.53 -0.1 464,851 + EX31_RS23475 0.60 +0.4 464,851 + EX31_RS23475 0.60 -0.3 464,875 + EX31_RS23475 0.66 -0.3 464,875 + EX31_RS23475 0.66 -0.3 464,875 + EX31_RS23475 0.66 -1.9 464,980 + -1.2 464,980 + +0.0 464,980 + -1.8 464,980 + +0.7 464,981 - -0.8 464,986 + +0.4 464,986 + +0.4 464,986 + -0.5 464,986 + -0.4 465,001 + -0.3 465,001 + -0.1 465,001 + +0.2 465,001 + +0.5
Or see this region's nucleotide sequence