Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS23310 and EX31_RS23315 are separated by 241 nucleotides EX31_RS23315 and EX31_RS23320 are separated by 24 nucleotides EX31_RS23320 and EX31_RS23325 are separated by 182 nucleotides EX31_RS23325 and EX31_RS23330 overlap by 11 nucleotides
EX31_RS23310: EX31_RS23310 - esterase, at 422,916 to 423,698
_RS23310
EX31_RS23315: EX31_RS23315 - replication initiation negative regulator SeqA, at 423,940 to 424,494
_RS23315
EX31_RS23320: EX31_RS23320 - alpha-D-glucose phosphate-specific phosphoglucomutase, at 424,519 to 426,162
_RS23320
EX31_RS23325: EX31_RS23325 - PadR family transcriptional regulator, at 426,345 to 427,061
_RS23325
EX31_RS23330: EX31_RS23330 - two-component system response regulator KdpE, at 427,051 to 427,740
_RS23330
Position (kb) 424
425
426
427 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 423.530 kb on + strand, within EX31_RS23310 at 423.554 kb on + strand, within EX31_RS23310 at 423.555 kb on - strand, within EX31_RS23310 at 423.594 kb on - strand, within EX31_RS23310 at 423.678 kb on + strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.679 kb on - strand at 423.699 kb on - strand at 423.699 kb on - strand at 423.704 kb on + strand at 423.717 kb on + strand at 423.717 kb on + strand at 423.717 kb on + strand at 423.717 kb on + strand at 423.722 kb on + strand at 423.788 kb on + strand at 423.894 kb on - strand at 423.913 kb on + strand at 423.913 kb on + strand at 423.913 kb on + strand at 423.913 kb on + strand at 423.913 kb on + strand at 423.913 kb on + strand at 423.948 kb on + strand at 423.976 kb on + strand at 423.976 kb on + strand at 424.032 kb on + strand, within EX31_RS23315 at 424.032 kb on + strand, within EX31_RS23315 at 424.033 kb on - strand, within EX31_RS23315 at 424.044 kb on + strand, within EX31_RS23315 at 424.070 kb on + strand, within EX31_RS23315 at 424.221 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.243 kb on + strand, within EX31_RS23315 at 424.244 kb on - strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.306 kb on + strand, within EX31_RS23315 at 424.350 kb on + strand, within EX31_RS23315 at 424.350 kb on + strand, within EX31_RS23315 at 424.359 kb on + strand, within EX31_RS23315 at 424.389 kb on + strand, within EX31_RS23315 at 424.392 kb on + strand, within EX31_RS23315 at 424.392 kb on + strand, within EX31_RS23315 at 424.413 kb on + strand, within EX31_RS23315 at 424.413 kb on + strand, within EX31_RS23315 at 424.594 kb on + strand at 424.594 kb on + strand at 424.594 kb on + strand at 424.595 kb on - strand at 424.595 kb on - strand at 424.595 kb on - strand at 424.595 kb on - strand at 424.597 kb on + strand at 424.597 kb on + strand at 424.597 kb on + strand at 424.597 kb on + strand at 424.698 kb on + strand, within EX31_RS23320 at 424.763 kb on - strand, within EX31_RS23320 at 424.987 kb on + strand, within EX31_RS23320 at 424.987 kb on + strand, within EX31_RS23320 at 424.988 kb on - strand, within EX31_RS23320 at 424.988 kb on - strand, within EX31_RS23320 at 424.988 kb on - strand, within EX31_RS23320 at 425.424 kb on + strand, within EX31_RS23320 at 425.437 kb on + strand, within EX31_RS23320 at 425.736 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.815 kb on + strand, within EX31_RS23320 at 425.851 kb on + strand, within EX31_RS23320 at 425.852 kb on - strand, within EX31_RS23320 at 425.859 kb on + strand, within EX31_RS23320 at 425.860 kb on - strand, within EX31_RS23320 at 426.061 kb on + strand at 426.061 kb on + strand at 426.061 kb on + strand at 426.061 kb on + strand at 426.070 kb on + strand at 426.070 kb on + strand at 426.156 kb on + strand at 426.156 kb on + strand at 426.160 kb on + strand at 426.160 kb on + strand at 426.160 kb on + strand at 426.160 kb on + strand at 426.160 kb on + strand at 426.196 kb on - strand at 426.251 kb on + strand at 426.251 kb on + strand at 426.251 kb on + strand at 426.251 kb on + strand at 426.251 kb on + strand at 426.251 kb on + strand at 426.252 kb on - strand at 426.255 kb on + strand at 426.256 kb on - strand at 426.295 kb on + strand at 426.322 kb on + strand at 426.328 kb on + strand at 426.329 kb on - strand at 426.329 kb on - strand at 426.339 kb on + strand at 426.339 kb on + strand at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.562 kb on + strand, within EX31_RS23325 at 426.636 kb on + strand, within EX31_RS23325 at 426.678 kb on + strand, within EX31_RS23325 at 426.678 kb on + strand, within EX31_RS23325 at 426.679 kb on - strand, within EX31_RS23325 at 426.682 kb on + strand, within EX31_RS23325 at 426.682 kb on + strand, within EX31_RS23325 at 427.053 kb on - strand at 427.053 kb on - strand at 427.059 kb on + strand at 427.060 kb on - strand at 427.060 kb on - strand at 427.091 kb on - strand at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.145 kb on + strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330 at 427.146 kb on - strand, within EX31_RS23330
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 423,530 + EX31_RS23310 0.78 +0.4 423,554 + EX31_RS23310 0.81 +0.9 423,555 - EX31_RS23310 0.82 -1.8 423,594 - EX31_RS23310 0.87 -1.6 423,678 + -1.9 423,679 - -0.1 423,679 - +0.5 423,679 - +0.6 423,679 - +0.4 423,679 - +0.3 423,679 - -0.7 423,679 - -1.1 423,699 - -0.2 423,699 - -2.0 423,704 + +0.2 423,717 + -0.3 423,717 + -0.4 423,717 + +1.0 423,717 + -0.9 423,722 + +0.4 423,788 + +0.3 423,894 - -0.1 423,913 + -0.9 423,913 + +1.0 423,913 + +0.1 423,913 + +0.1 423,913 + +0.4 423,913 + -0.8 423,948 + +0.9 423,976 + +0.4 423,976 + -1.5 424,032 + EX31_RS23315 0.17 -0.9 424,032 + EX31_RS23315 0.17 -0.3 424,033 - EX31_RS23315 0.17 +0.5 424,044 + EX31_RS23315 0.19 -0.4 424,070 + EX31_RS23315 0.23 +0.3 424,221 + EX31_RS23315 0.51 +0.2 424,243 + EX31_RS23315 0.55 +1.7 424,243 + EX31_RS23315 0.55 -0.7 424,243 + EX31_RS23315 0.55 -2.2 424,243 + EX31_RS23315 0.55 -0.7 424,243 + EX31_RS23315 0.55 +0.5 424,243 + EX31_RS23315 0.55 +0.7 424,244 - EX31_RS23315 0.55 -1.6 424,306 + EX31_RS23315 0.66 -0.7 424,306 + EX31_RS23315 0.66 +0.6 424,306 + EX31_RS23315 0.66 -1.8 424,306 + EX31_RS23315 0.66 -3.1 424,306 + EX31_RS23315 0.66 +0.9 424,306 + EX31_RS23315 0.66 -0.1 424,350 + EX31_RS23315 0.74 -2.9 424,350 + EX31_RS23315 0.74 -1.3 424,359 + EX31_RS23315 0.75 +0.9 424,389 + EX31_RS23315 0.81 -1.4 424,392 + EX31_RS23315 0.81 +0.3 424,392 + EX31_RS23315 0.81 -0.1 424,413 + EX31_RS23315 0.85 +0.7 424,413 + EX31_RS23315 0.85 -0.4 424,594 + -2.3 424,594 + -2.1 424,594 + +0.2 424,595 - -1.5 424,595 - -1.3 424,595 - -0.8 424,595 - -1.0 424,597 + -0.9 424,597 + -1.0 424,597 + -1.5 424,597 + -1.5 424,698 + EX31_RS23320 0.11 -1.6 424,763 - EX31_RS23320 0.15 +1.0 424,987 + EX31_RS23320 0.28 -0.7 424,987 + EX31_RS23320 0.28 -0.9 424,988 - EX31_RS23320 0.29 -0.4 424,988 - EX31_RS23320 0.29 +0.4 424,988 - EX31_RS23320 0.29 -1.1 425,424 + EX31_RS23320 0.55 +0.9 425,437 + EX31_RS23320 0.56 -2.5 425,736 + EX31_RS23320 0.74 -0.9 425,815 + EX31_RS23320 0.79 -2.6 425,815 + EX31_RS23320 0.79 -1.2 425,815 + EX31_RS23320 0.79 +0.2 425,815 + EX31_RS23320 0.79 -0.3 425,815 + EX31_RS23320 0.79 -1.3 425,815 + EX31_RS23320 0.79 -2.9 425,851 + EX31_RS23320 0.81 -1.7 425,852 - EX31_RS23320 0.81 +0.0 425,859 + EX31_RS23320 0.82 -2.7 425,860 - EX31_RS23320 0.82 +0.0 426,061 + -0.6 426,061 + -1.8 426,061 + -1.8 426,061 + +2.2 426,070 + -0.6 426,070 + -1.3 426,156 + +0.1 426,156 + -1.9 426,160 + +0.5 426,160 + -1.5 426,160 + -0.1 426,160 + +0.7 426,160 + +0.9 426,196 - +0.5 426,251 + +0.1 426,251 + +1.1 426,251 + -0.1 426,251 + +0.8 426,251 + -1.3 426,251 + +0.5 426,252 - -0.1 426,255 + -0.6 426,256 - +0.1 426,295 + +0.3 426,322 + -0.6 426,328 + -0.3 426,329 - +0.6 426,329 - -0.4 426,339 + -0.5 426,339 + +0.1 426,562 + EX31_RS23325 0.30 +0.4 426,562 + EX31_RS23325 0.30 +0.4 426,562 + EX31_RS23325 0.30 +0.4 426,562 + EX31_RS23325 0.30 +1.2 426,562 + EX31_RS23325 0.30 +0.6 426,562 + EX31_RS23325 0.30 -0.1 426,562 + EX31_RS23325 0.30 -1.0 426,636 + EX31_RS23325 0.41 +0.0 426,678 + EX31_RS23325 0.46 +0.7 426,678 + EX31_RS23325 0.46 +0.1 426,679 - EX31_RS23325 0.47 +1.4 426,682 + EX31_RS23325 0.47 +0.1 426,682 + EX31_RS23325 0.47 +0.4 427,053 - -0.8 427,053 - -0.2 427,059 + +0.1 427,060 - +0.7 427,060 - -2.2 427,091 - -2.1 427,145 + EX31_RS23330 0.14 -0.7 427,145 + EX31_RS23330 0.14 -1.3 427,145 + EX31_RS23330 0.14 -0.4 427,145 + EX31_RS23330 0.14 -1.2 427,145 + EX31_RS23330 0.14 +0.4 427,145 + EX31_RS23330 0.14 -0.1 427,145 + EX31_RS23330 0.14 +0.4 427,145 + EX31_RS23330 0.14 +0.7 427,145 + EX31_RS23330 0.14 -2.7 427,145 + EX31_RS23330 0.14 -0.3 427,145 + EX31_RS23330 0.14 -2.5 427,145 + EX31_RS23330 0.14 +2.1 427,146 - EX31_RS23330 0.14 -1.2 427,146 - EX31_RS23330 0.14 +1.2 427,146 - EX31_RS23330 0.14 -1.6 427,146 - EX31_RS23330 0.14 +0.4 427,146 - EX31_RS23330 0.14 -1.8 427,146 - EX31_RS23330 0.14 -0.6 427,146 - EX31_RS23330 0.14 +0.4 427,146 - EX31_RS23330 0.14 +0.2 427,146 - EX31_RS23330 0.14 -1.0 427,146 - EX31_RS23330 0.14 +0.2 427,146 - EX31_RS23330 0.14 -1.0 427,146 - EX31_RS23330 0.14 +0.5
Or see this region's nucleotide sequence