Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS21775 and EX31_RS21780 are separated by 339 nucleotides EX31_RS21780 and EX31_RS21785 are separated by 92 nucleotides EX31_RS21785 and EX31_RS21790 are separated by 156 nucleotides EX31_RS21790 and EX31_RS21795 are separated by 63 nucleotides
EX31_RS21775: EX31_RS21775 - uracil-DNA glycosylase, at 98,154 to 98,834
_RS21775
EX31_RS21780: EX31_RS21780 - autonomous glycyl radical cofactor GrcA, at 99,174 to 99,557
_RS21780
EX31_RS21785: EX31_RS21785 - ATP-dependent RNA helicase SrmB, at 99,650 to 100,978
_RS21785
EX31_RS21790: EX31_RS21790 - tRNA1(Val) (adenine(37)-N6)-methyltransferase, at 101,135 to 101,881
_RS21790
EX31_RS21795: EX31_RS21795 - L-aspartate oxidase, at 101,945 to 103,582
_RS21795
Position (kb) 99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 98.699 kb on + strand, within EX31_RS21775 at 98.699 kb on + strand, within EX31_RS21775 at 98.722 kb on + strand, within EX31_RS21775 at 98.769 kb on - strand at 98.779 kb on + strand at 98.779 kb on + strand at 98.779 kb on + strand at 98.779 kb on + strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.780 kb on - strand at 98.855 kb on + strand at 98.877 kb on + strand at 98.877 kb on + strand at 98.878 kb on - strand at 98.926 kb on + strand at 98.927 kb on - strand at 98.937 kb on + strand at 98.937 kb on + strand at 98.937 kb on + strand at 98.938 kb on - strand at 98.938 kb on - strand at 98.938 kb on - strand at 99.009 kb on - strand at 99.015 kb on + strand at 99.015 kb on + strand at 99.015 kb on + strand at 99.015 kb on + strand at 99.015 kb on + strand at 99.016 kb on - strand at 99.016 kb on - strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.019 kb on + strand at 99.037 kb on + strand at 99.059 kb on - strand at 99.090 kb on + strand at 99.090 kb on + strand at 99.090 kb on + strand at 99.090 kb on + strand at 99.092 kb on + strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.093 kb on - strand at 99.128 kb on + strand at 99.128 kb on + strand at 99.128 kb on + strand at 99.128 kb on + strand at 99.128 kb on + strand at 99.129 kb on - strand at 99.129 kb on - strand at 99.129 kb on - strand at 99.129 kb on - strand at 99.141 kb on + strand at 99.141 kb on + strand at 99.142 kb on - strand at 99.142 kb on - strand at 99.142 kb on - strand at 99.147 kb on + strand at 99.147 kb on + strand at 99.147 kb on + strand at 99.152 kb on + strand at 99.152 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.173 kb on + strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.174 kb on - strand at 99.194 kb on + strand at 99.195 kb on - strand at 99.195 kb on - strand at 99.198 kb on - strand at 99.334 kb on - strand, within EX31_RS21780 at 99.343 kb on + strand, within EX31_RS21780 at 99.343 kb on + strand, within EX31_RS21780 at 99.343 kb on + strand, within EX31_RS21780 at 99.344 kb on - strand, within EX31_RS21780 at 99.367 kb on + strand, within EX31_RS21780 at 99.367 kb on + strand, within EX31_RS21780 at 99.367 kb on + strand, within EX31_RS21780 at 99.367 kb on + strand, within EX31_RS21780 at 99.367 kb on + strand, within EX31_RS21780 at 99.368 kb on - strand, within EX31_RS21780 at 99.368 kb on - strand, within EX31_RS21780 at 99.403 kb on + strand, within EX31_RS21780 at 99.403 kb on + strand, within EX31_RS21780 at 99.403 kb on + strand, within EX31_RS21780 at 99.494 kb on + strand, within EX31_RS21780 at 99.495 kb on - strand, within EX31_RS21780 at 99.495 kb on - strand, within EX31_RS21780 at 99.536 kb on + strand at 99.537 kb on - strand at 99.596 kb on + strand at 99.597 kb on - strand at 99.598 kb on + strand at 99.599 kb on - strand at 99.599 kb on - strand at 99.652 kb on - strand at 99.652 kb on - strand at 99.655 kb on + strand at 99.727 kb on + strand at 99.728 kb on - strand at 99.728 kb on - strand at 99.803 kb on - strand, within EX31_RS21785 at 99.803 kb on - strand, within EX31_RS21785 at 99.878 kb on - strand, within EX31_RS21785 at 99.883 kb on - strand, within EX31_RS21785 at 99.947 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.985 kb on - strand, within EX31_RS21785 at 99.989 kb on - strand, within EX31_RS21785 at 99.989 kb on - strand, within EX31_RS21785 at 99.989 kb on - strand, within EX31_RS21785 at 99.989 kb on - strand, within EX31_RS21785 at 99.989 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.100 kb on - strand, within EX31_RS21785 at 100.150 kb on - strand, within EX31_RS21785 at 100.159 kb on + strand, within EX31_RS21785 at 100.160 kb on - strand, within EX31_RS21785 at 100.160 kb on - strand, within EX31_RS21785 at 100.160 kb on - strand, within EX31_RS21785 at 100.160 kb on - strand, within EX31_RS21785 at 100.160 kb on - strand, within EX31_RS21785 at 100.211 kb on - strand, within EX31_RS21785 at 100.277 kb on - strand, within EX31_RS21785 at 100.556 kb on - strand, within EX31_RS21785 at 100.561 kb on - strand, within EX31_RS21785 at 100.561 kb on - strand, within EX31_RS21785 at 100.630 kb on + strand, within EX31_RS21785 at 100.630 kb on + strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.631 kb on - strand, within EX31_RS21785 at 100.632 kb on + strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.633 kb on - strand, within EX31_RS21785 at 100.673 kb on - strand, within EX31_RS21785 at 100.673 kb on - strand, within EX31_RS21785 at 100.673 kb on - strand, within EX31_RS21785 at 100.897 kb on + strand at 100.907 kb on - strand at 100.909 kb on - strand at 100.971 kb on - strand at 100.989 kb on + strand at 100.990 kb on - strand at 100.990 kb on - strand at 101.005 kb on + strand at 101.007 kb on + strand at 101.008 kb on - strand at 101.008 kb on - strand at 101.008 kb on - strand at 101.016 kb on + strand at 101.033 kb on + strand at 101.033 kb on + strand at 101.033 kb on + strand at 101.034 kb on - strand at 101.034 kb on - strand at 101.034 kb on - strand at 101.034 kb on - strand at 101.034 kb on - strand at 101.037 kb on + strand at 101.037 kb on + strand at 101.038 kb on - strand at 101.038 kb on - strand at 101.038 kb on - strand at 101.038 kb on - strand at 101.038 kb on - strand at 101.057 kb on + strand at 101.057 kb on + strand at 101.058 kb on - strand at 101.077 kb on + strand at 101.077 kb on + strand at 101.077 kb on + strand at 101.078 kb on - strand at 101.078 kb on - strand at 101.078 kb on - strand at 101.082 kb on + strand at 101.083 kb on - strand at 101.083 kb on - strand at 101.098 kb on + strand at 101.099 kb on - strand at 101.099 kb on - strand at 101.099 kb on - strand at 101.099 kb on - strand at 101.118 kb on + strand at 101.118 kb on + strand at 101.118 kb on + strand at 101.122 kb on + strand at 101.122 kb on + strand at 101.123 kb on - strand at 101.146 kb on - strand at 101.166 kb on + strand at 101.166 kb on + strand at 101.183 kb on - strand at 101.188 kb on + strand at 101.189 kb on - strand at 101.189 kb on - strand at 101.294 kb on - strand, within EX31_RS21790 at 101.342 kb on - strand, within EX31_RS21790 at 101.342 kb on - strand, within EX31_RS21790 at 101.444 kb on + strand, within EX31_RS21790 at 101.444 kb on + strand, within EX31_RS21790 at 101.445 kb on - strand, within EX31_RS21790 at 101.445 kb on - strand, within EX31_RS21790 at 101.445 kb on - strand, within EX31_RS21790 at 101.459 kb on - strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.465 kb on + strand, within EX31_RS21790 at 101.466 kb on - strand, within EX31_RS21790 at 101.490 kb on - strand, within EX31_RS21790 at 101.517 kb on - strand, within EX31_RS21790 at 101.517 kb on - strand, within EX31_RS21790 at 101.611 kb on + strand, within EX31_RS21790 at 101.611 kb on + strand, within EX31_RS21790 at 101.611 kb on + strand, within EX31_RS21790 at 101.611 kb on + strand, within EX31_RS21790 at 101.611 kb on + strand, within EX31_RS21790 at 101.612 kb on - strand, within EX31_RS21790 at 101.612 kb on - strand, within EX31_RS21790 at 101.621 kb on - strand, within EX31_RS21790 at 101.638 kb on + strand, within EX31_RS21790 at 101.639 kb on - strand, within EX31_RS21790 at 101.639 kb on - strand, within EX31_RS21790 at 101.654 kb on + strand, within EX31_RS21790 at 101.655 kb on - strand, within EX31_RS21790 at 101.769 kb on + strand, within EX31_RS21790 at 101.770 kb on - strand, within EX31_RS21790 at 101.770 kb on - strand, within EX31_RS21790 at 101.831 kb on + strand at 101.943 kb on + strand at 101.943 kb on + strand at 101.943 kb on + strand at 101.944 kb on - strand at 101.944 kb on - strand at 101.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 98,699 + EX31_RS21775 0.80 -0.9 98,699 + EX31_RS21775 0.80 +0.3 98,722 + EX31_RS21775 0.83 +0.0 98,769 - +0.3 98,779 + -0.0 98,779 + +0.1 98,779 + -0.3 98,779 + +1.1 98,780 - -1.8 98,780 - +0.3 98,780 - -0.6 98,780 - -0.1 98,780 - +0.4 98,780 - -1.9 98,780 - -0.2 98,780 - +0.4 98,855 + -0.8 98,877 + -1.0 98,877 + +0.4 98,878 - +0.1 98,926 + -0.4 98,927 - +0.5 98,937 + +0.0 98,937 + -1.7 98,937 + -0.2 98,938 - -1.6 98,938 - -0.8 98,938 - +0.1 99,009 - -0.2 99,015 + +1.0 99,015 + +0.2 99,015 + -0.7 99,015 + +0.6 99,015 + +0.8 99,016 - +0.6 99,016 - -1.8 99,019 + -0.1 99,019 + +1.4 99,019 + -0.1 99,019 + -0.1 99,019 + -0.8 99,019 + +0.1 99,037 + -0.1 99,059 - -1.0 99,090 + -0.3 99,090 + +0.5 99,090 + +0.3 99,090 + -2.5 99,092 + -0.5 99,093 - +0.6 99,093 - +0.3 99,093 - +0.2 99,093 - +0.4 99,093 - +0.1 99,093 - +1.3 99,093 - -1.6 99,093 - +1.4 99,128 + -0.7 99,128 + -0.1 99,128 + +1.0 99,128 + +0.4 99,128 + -0.0 99,129 - +1.2 99,129 - +0.2 99,129 - -0.0 99,129 - +1.3 99,141 + -0.2 99,141 + +0.4 99,142 - +0.4 99,142 - -0.4 99,142 - +0.4 99,147 + +0.9 99,147 + -0.2 99,147 + +0.2 99,152 + +0.1 99,152 + +0.8 99,173 + -0.0 99,173 + +0.0 99,173 + +0.2 99,173 + -0.9 99,173 + +0.4 99,173 + +0.8 99,173 + -1.0 99,173 + -0.6 99,173 + +0.7 99,173 + +0.5 99,174 - -1.0 99,174 - -0.7 99,174 - -0.3 99,174 - +0.1 99,174 - +1.4 99,174 - +1.0 99,174 - +0.0 99,174 - +1.2 99,174 - +0.9 99,174 - -0.5 99,194 + -1.3 99,195 - -1.1 99,195 - +0.1 99,198 - +0.3 99,334 - EX31_RS21780 0.42 -0.7 99,343 + EX31_RS21780 0.44 +0.2 99,343 + EX31_RS21780 0.44 +1.0 99,343 + EX31_RS21780 0.44 -0.2 99,344 - EX31_RS21780 0.44 -1.2 99,367 + EX31_RS21780 0.50 +0.6 99,367 + EX31_RS21780 0.50 +0.6 99,367 + EX31_RS21780 0.50 +1.8 99,367 + EX31_RS21780 0.50 +0.3 99,367 + EX31_RS21780 0.50 -1.2 99,368 - EX31_RS21780 0.51 +1.3 99,368 - EX31_RS21780 0.51 -0.5 99,403 + EX31_RS21780 0.60 +0.5 99,403 + EX31_RS21780 0.60 -1.4 99,403 + EX31_RS21780 0.60 -1.3 99,494 + EX31_RS21780 0.83 -0.0 99,495 - EX31_RS21780 0.84 +0.9 99,495 - EX31_RS21780 0.84 -0.4 99,536 + +0.8 99,537 - +0.0 99,596 + +0.8 99,597 - +0.1 99,598 + +0.4 99,599 - +0.1 99,599 - -0.4 99,652 - +0.1 99,652 - +0.8 99,655 + -0.7 99,727 + -0.1 99,728 - +0.3 99,728 - +0.8 99,803 - EX31_RS21785 0.12 -0.9 99,803 - EX31_RS21785 0.12 -0.4 99,878 - EX31_RS21785 0.17 -1.1 99,883 - EX31_RS21785 0.18 -1.5 99,947 - EX31_RS21785 0.22 +0.1 99,985 - EX31_RS21785 0.25 +0.8 99,985 - EX31_RS21785 0.25 -0.5 99,985 - EX31_RS21785 0.25 +0.2 99,985 - EX31_RS21785 0.25 -0.6 99,985 - EX31_RS21785 0.25 +1.3 99,985 - EX31_RS21785 0.25 +0.1 99,989 - EX31_RS21785 0.26 +1.3 99,989 - EX31_RS21785 0.26 -0.9 99,989 - EX31_RS21785 0.26 -1.5 99,989 - EX31_RS21785 0.26 -1.4 99,989 - EX31_RS21785 0.26 +0.4 100,100 - EX31_RS21785 0.34 -0.1 100,100 - EX31_RS21785 0.34 -1.9 100,100 - EX31_RS21785 0.34 -1.9 100,100 - EX31_RS21785 0.34 -2.6 100,100 - EX31_RS21785 0.34 +0.1 100,100 - EX31_RS21785 0.34 -0.1 100,150 - EX31_RS21785 0.38 +0.3 100,159 + EX31_RS21785 0.38 -0.1 100,160 - EX31_RS21785 0.38 -0.6 100,160 - EX31_RS21785 0.38 -2.6 100,160 - EX31_RS21785 0.38 -1.0 100,160 - EX31_RS21785 0.38 -2.8 100,160 - EX31_RS21785 0.38 +0.0 100,211 - EX31_RS21785 0.42 -1.7 100,277 - EX31_RS21785 0.47 -1.6 100,556 - EX31_RS21785 0.68 -0.7 100,561 - EX31_RS21785 0.69 +0.2 100,561 - EX31_RS21785 0.69 +1.8 100,630 + EX31_RS21785 0.74 -0.4 100,630 + EX31_RS21785 0.74 -1.6 100,631 - EX31_RS21785 0.74 -1.9 100,631 - EX31_RS21785 0.74 +1.1 100,631 - EX31_RS21785 0.74 -1.5 100,631 - EX31_RS21785 0.74 +0.3 100,631 - EX31_RS21785 0.74 -1.2 100,631 - EX31_RS21785 0.74 -0.4 100,632 + EX31_RS21785 0.74 -0.3 100,633 - EX31_RS21785 0.74 -1.1 100,633 - EX31_RS21785 0.74 +0.2 100,633 - EX31_RS21785 0.74 -0.4 100,633 - EX31_RS21785 0.74 -1.8 100,633 - EX31_RS21785 0.74 -2.4 100,633 - EX31_RS21785 0.74 -1.3 100,633 - EX31_RS21785 0.74 -0.0 100,633 - EX31_RS21785 0.74 -0.6 100,633 - EX31_RS21785 0.74 -1.0 100,673 - EX31_RS21785 0.77 -1.3 100,673 - EX31_RS21785 0.77 -0.6 100,673 - EX31_RS21785 0.77 +0.2 100,897 + -0.6 100,907 - -1.3 100,909 - -2.3 100,971 - -1.1 100,989 + -1.1 100,990 - -0.1 100,990 - +0.8 101,005 + -1.4 101,007 + -0.1 101,008 - +0.4 101,008 - +0.9 101,008 - +0.6 101,016 + -1.9 101,033 + +0.1 101,033 + +0.4 101,033 + -2.5 101,034 - -1.6 101,034 - +0.8 101,034 - +0.6 101,034 - -0.3 101,034 - -0.5 101,037 + +0.2 101,037 + +0.4 101,038 - +0.6 101,038 - +0.2 101,038 - +0.8 101,038 - -0.2 101,038 - -2.5 101,057 + +0.6 101,057 + -0.3 101,058 - -0.3 101,077 + +0.6 101,077 + -0.0 101,077 + -0.6 101,078 - -0.7 101,078 - -0.0 101,078 - -0.2 101,082 + +0.3 101,083 - +0.2 101,083 - -0.2 101,098 + -0.3 101,099 - +0.9 101,099 - -0.3 101,099 - -2.1 101,099 - -1.4 101,118 + -0.8 101,118 + -0.1 101,118 + -0.4 101,122 + +0.7 101,122 + -0.1 101,123 - +0.7 101,146 - -0.2 101,166 + +0.7 101,166 + +1.0 101,183 - +0.2 101,188 + -0.7 101,189 - -2.0 101,189 - +0.7 101,294 - EX31_RS21790 0.21 -1.2 101,342 - EX31_RS21790 0.28 -0.2 101,342 - EX31_RS21790 0.28 -0.2 101,444 + EX31_RS21790 0.41 -0.2 101,444 + EX31_RS21790 0.41 +0.6 101,445 - EX31_RS21790 0.41 +0.2 101,445 - EX31_RS21790 0.41 +0.1 101,445 - EX31_RS21790 0.41 -0.9 101,459 - EX31_RS21790 0.43 +0.1 101,465 + EX31_RS21790 0.44 +0.5 101,465 + EX31_RS21790 0.44 +1.3 101,465 + EX31_RS21790 0.44 -2.5 101,465 + EX31_RS21790 0.44 +0.0 101,465 + EX31_RS21790 0.44 +0.4 101,465 + EX31_RS21790 0.44 -1.2 101,465 + EX31_RS21790 0.44 -0.5 101,466 - EX31_RS21790 0.44 +0.6 101,490 - EX31_RS21790 0.48 -0.5 101,517 - EX31_RS21790 0.51 -0.7 101,517 - EX31_RS21790 0.51 -0.0 101,611 + EX31_RS21790 0.64 -1.1 101,611 + EX31_RS21790 0.64 +0.4 101,611 + EX31_RS21790 0.64 -0.1 101,611 + EX31_RS21790 0.64 -0.6 101,611 + EX31_RS21790 0.64 +1.1 101,612 - EX31_RS21790 0.64 -0.2 101,612 - EX31_RS21790 0.64 +0.2 101,621 - EX31_RS21790 0.65 +1.3 101,638 + EX31_RS21790 0.67 -0.1 101,639 - EX31_RS21790 0.67 +0.4 101,639 - EX31_RS21790 0.67 -0.4 101,654 + EX31_RS21790 0.69 +0.1 101,655 - EX31_RS21790 0.70 -0.8 101,769 + EX31_RS21790 0.85 -0.6 101,770 - EX31_RS21790 0.85 -1.1 101,770 - EX31_RS21790 0.85 -0.4 101,831 + +0.7 101,943 + -0.8 101,943 + -0.6 101,943 + -0.0 101,944 - +0.7 101,944 - +0.9 101,947 - +0.7
Or see this region's nucleotide sequence