Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS10035 and EX31_RS10040 are separated by 80 nucleotides EX31_RS10040 and EX31_RS10045 are separated by 181 nucleotides EX31_RS10045 and EX31_RS10050 overlap by 4 nucleotides
EX31_RS10035: EX31_RS10035 - cardiolipin synthase, at 213,041 to 214,501
_RS10035
EX31_RS10040: EX31_RS10040 - YciU family protein, at 214,582 to 214,905
_RS10040
EX31_RS10045: EX31_RS10045 - ABC transporter ATP-binding protein, at 215,087 to 216,124
_RS10045
EX31_RS10050: EX31_RS10050 - ABC transporter ATP-binding protein, at 216,121 to 217,137
_RS10050
Position (kb) 215
216
217 Strain fitness (log2 ratio) -2
-1
0
1 at 214.500 kb on - strand at 214.522 kb on + strand at 214.522 kb on + strand at 214.522 kb on + strand at 214.525 kb on + strand at 214.525 kb on + strand at 214.525 kb on + strand at 214.581 kb on + strand at 214.582 kb on - strand at 214.724 kb on + strand, within EX31_RS10040 at 214.724 kb on + strand, within EX31_RS10040 at 214.724 kb on + strand, within EX31_RS10040 at 214.724 kb on + strand, within EX31_RS10040 at 214.724 kb on + strand, within EX31_RS10040 at 214.724 kb on + strand, within EX31_RS10040 at 214.726 kb on + strand, within EX31_RS10040 at 214.727 kb on - strand, within EX31_RS10040 at 214.727 kb on - strand, within EX31_RS10040 at 214.727 kb on - strand, within EX31_RS10040 at 214.827 kb on + strand, within EX31_RS10040 at 214.827 kb on + strand, within EX31_RS10040 at 214.827 kb on + strand, within EX31_RS10040 at 214.827 kb on + strand, within EX31_RS10040 at 214.828 kb on - strand, within EX31_RS10040 at 214.828 kb on - strand, within EX31_RS10040 at 214.836 kb on + strand, within EX31_RS10040 at 214.903 kb on + strand at 214.904 kb on - strand at 214.984 kb on + strand at 214.985 kb on - strand at 214.985 kb on - strand at 214.995 kb on + strand at 214.995 kb on + strand at 214.995 kb on + strand at 215.082 kb on - strand at 215.216 kb on - strand, within EX31_RS10045 at 215.229 kb on - strand, within EX31_RS10045 at 215.314 kb on - strand, within EX31_RS10045 at 215.347 kb on - strand, within EX31_RS10045 at 215.347 kb on - strand, within EX31_RS10045 at 215.347 kb on - strand, within EX31_RS10045 at 215.376 kb on + strand, within EX31_RS10045 at 215.376 kb on + strand, within EX31_RS10045 at 215.377 kb on - strand, within EX31_RS10045 at 215.377 kb on - strand, within EX31_RS10045 at 215.377 kb on - strand, within EX31_RS10045 at 215.408 kb on + strand, within EX31_RS10045 at 215.646 kb on - strand, within EX31_RS10045 at 215.699 kb on - strand, within EX31_RS10045 at 215.700 kb on + strand, within EX31_RS10045 at 215.701 kb on - strand, within EX31_RS10045 at 215.822 kb on - strand, within EX31_RS10045 at 215.950 kb on - strand, within EX31_RS10045 at 216.145 kb on - strand at 216.145 kb on - strand at 216.145 kb on - strand at 216.146 kb on + strand at 216.146 kb on + strand at 216.147 kb on - strand at 216.147 kb on - strand at 216.147 kb on - strand at 216.147 kb on - strand at 216.147 kb on - strand at 216.147 kb on - strand at 216.363 kb on - strand, within EX31_RS10050 at 216.387 kb on - strand, within EX31_RS10050 at 216.407 kb on + strand, within EX31_RS10050 at 216.407 kb on + strand, within EX31_RS10050 at 216.408 kb on - strand, within EX31_RS10050 at 216.432 kb on + strand, within EX31_RS10050 at 216.459 kb on + strand, within EX31_RS10050 at 216.460 kb on - strand, within EX31_RS10050 at 216.460 kb on - strand, within EX31_RS10050 at 216.466 kb on - strand, within EX31_RS10050 at 216.466 kb on - strand, within EX31_RS10050 at 216.573 kb on + strand, within EX31_RS10050 at 216.694 kb on + strand, within EX31_RS10050 at 216.695 kb on - strand, within EX31_RS10050 at 216.721 kb on - strand, within EX31_RS10050 at 216.838 kb on - strand, within EX31_RS10050 at 216.910 kb on - strand, within EX31_RS10050 at 216.952 kb on - strand, within EX31_RS10050 at 216.952 kb on - strand, within EX31_RS10050 at 217.078 kb on + strand at 217.079 kb on - strand at 217.079 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 214,500 - -0.1 214,522 + -1.5 214,522 + -0.7 214,522 + -0.5 214,525 + +0.0 214,525 + +0.5 214,525 + +0.6 214,581 + -0.4 214,582 - +0.5 214,724 + EX31_RS10040 0.44 -0.8 214,724 + EX31_RS10040 0.44 -1.3 214,724 + EX31_RS10040 0.44 -2.0 214,724 + EX31_RS10040 0.44 +1.4 214,724 + EX31_RS10040 0.44 -0.9 214,724 + EX31_RS10040 0.44 +0.4 214,726 + EX31_RS10040 0.44 -0.5 214,727 - EX31_RS10040 0.45 -0.9 214,727 - EX31_RS10040 0.45 -1.2 214,727 - EX31_RS10040 0.45 +0.2 214,827 + EX31_RS10040 0.76 -1.4 214,827 + EX31_RS10040 0.76 -0.3 214,827 + EX31_RS10040 0.76 +0.7 214,827 + EX31_RS10040 0.76 -1.0 214,828 - EX31_RS10040 0.76 -1.4 214,828 - EX31_RS10040 0.76 -1.1 214,836 + EX31_RS10040 0.78 -0.8 214,903 + +1.2 214,904 - -0.6 214,984 + +1.1 214,985 - -1.7 214,985 - -0.2 214,995 + -0.7 214,995 + +1.1 214,995 + +0.6 215,082 - +0.8 215,216 - EX31_RS10045 0.12 -1.1 215,229 - EX31_RS10045 0.14 +0.3 215,314 - EX31_RS10045 0.22 -0.9 215,347 - EX31_RS10045 0.25 -2.0 215,347 - EX31_RS10045 0.25 +1.0 215,347 - EX31_RS10045 0.25 +1.2 215,376 + EX31_RS10045 0.28 +0.0 215,376 + EX31_RS10045 0.28 -1.6 215,377 - EX31_RS10045 0.28 -1.1 215,377 - EX31_RS10045 0.28 -0.5 215,377 - EX31_RS10045 0.28 -0.2 215,408 + EX31_RS10045 0.31 -2.0 215,646 - EX31_RS10045 0.54 -0.2 215,699 - EX31_RS10045 0.59 +0.0 215,700 + EX31_RS10045 0.59 -1.8 215,701 - EX31_RS10045 0.59 -0.0 215,822 - EX31_RS10045 0.71 +0.8 215,950 - EX31_RS10045 0.83 +0.2 216,145 - -0.1 216,145 - -2.0 216,145 - +0.3 216,146 + -0.2 216,146 + +0.0 216,147 - +0.6 216,147 - +0.6 216,147 - -1.0 216,147 - -1.7 216,147 - +0.5 216,147 - +0.4 216,363 - EX31_RS10050 0.24 +0.4 216,387 - EX31_RS10050 0.26 +1.1 216,407 + EX31_RS10050 0.28 -1.8 216,407 + EX31_RS10050 0.28 +0.4 216,408 - EX31_RS10050 0.28 -0.6 216,432 + EX31_RS10050 0.31 +0.4 216,459 + EX31_RS10050 0.33 +1.0 216,460 - EX31_RS10050 0.33 +1.1 216,460 - EX31_RS10050 0.33 -0.5 216,466 - EX31_RS10050 0.34 +0.9 216,466 - EX31_RS10050 0.34 -0.1 216,573 + EX31_RS10050 0.44 +0.2 216,694 + EX31_RS10050 0.56 -0.5 216,695 - EX31_RS10050 0.56 +1.2 216,721 - EX31_RS10050 0.59 -0.8 216,838 - EX31_RS10050 0.71 -0.0 216,910 - EX31_RS10050 0.78 -0.5 216,952 - EX31_RS10050 0.82 -0.8 216,952 - EX31_RS10050 0.82 -0.5 217,078 + -1.3 217,079 - +0.1 217,079 - +0.1
Or see this region's nucleotide sequence