Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS06965 and EX31_RS06970 are separated by 246 nucleotides EX31_RS06970 and EX31_RS06975 are separated by 134 nucleotides
EX31_RS06965: EX31_RS06965 - class II fumarate hydratase, at 181,077 to 182,474
_RS06965
EX31_RS06970: EX31_RS06970 - mannose-6-phosphate isomerase, at 182,721 to 183,902
_RS06970
EX31_RS06975: EX31_RS06975 - YdgA family protein, at 184,037 to 185,578
_RS06975
Position (kb)
182
183
184 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 181.764 kb on - strand, within EX31_RS06965 at 181.812 kb on - strand, within EX31_RS06965 at 182.096 kb on + strand, within EX31_RS06965 at 182.159 kb on + strand, within EX31_RS06965 at 182.159 kb on + strand, within EX31_RS06965 at 182.160 kb on - strand, within EX31_RS06965 at 182.160 kb on - strand, within EX31_RS06965 at 182.298 kb on - strand, within EX31_RS06965 at 182.486 kb on + strand at 182.495 kb on - strand at 182.498 kb on - strand at 182.498 kb on - strand at 182.498 kb on - strand at 182.506 kb on - strand at 182.526 kb on + strand at 182.526 kb on + strand at 182.574 kb on + strand at 182.574 kb on + strand at 182.574 kb on + strand at 182.575 kb on - strand at 182.575 kb on - strand at 182.577 kb on - strand at 182.577 kb on - strand at 182.584 kb on + strand at 182.584 kb on + strand at 182.584 kb on + strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.585 kb on - strand at 182.586 kb on + strand at 182.586 kb on + strand at 182.586 kb on + strand at 182.587 kb on - strand at 182.587 kb on - strand at 182.587 kb on - strand at 182.597 kb on + strand at 182.599 kb on + strand at 182.599 kb on + strand at 182.600 kb on - strand at 182.600 kb on - strand at 182.663 kb on + strand at 182.663 kb on + strand at 182.688 kb on + strand at 182.689 kb on - strand at 182.698 kb on + strand at 182.698 kb on + strand at 182.706 kb on - strand at 182.735 kb on + strand at 182.787 kb on + strand at 182.795 kb on + strand at 182.853 kb on - strand, within EX31_RS06970 at 182.905 kb on + strand, within EX31_RS06970 at 182.905 kb on + strand, within EX31_RS06970 at 182.906 kb on - strand, within EX31_RS06970 at 183.097 kb on - strand, within EX31_RS06970 at 183.302 kb on + strand, within EX31_RS06970 at 183.302 kb on + strand, within EX31_RS06970 at 183.302 kb on + strand, within EX31_RS06970 at 183.378 kb on - strand, within EX31_RS06970 at 183.433 kb on + strand, within EX31_RS06970 at 183.434 kb on - strand, within EX31_RS06970 at 183.434 kb on - strand, within EX31_RS06970 at 183.434 kb on - strand, within EX31_RS06970 at 183.489 kb on + strand, within EX31_RS06970 at 183.562 kb on - strand, within EX31_RS06970 at 183.755 kb on + strand, within EX31_RS06970 at 183.756 kb on - strand, within EX31_RS06970 at 183.965 kb on + strand at 183.966 kb on - strand at 183.979 kb on + strand at 184.036 kb on + strand at 184.036 kb on + strand at 184.036 kb on + strand at 184.071 kb on + strand at 184.132 kb on + strand at 184.132 kb on + strand at 184.132 kb on + strand at 184.535 kb on + strand, within EX31_RS06975 at 184.723 kb on + strand, within EX31_RS06975 at 184.723 kb on + strand, within EX31_RS06975 at 184.810 kb on + strand, within EX31_RS06975 at 184.849 kb on + strand, within EX31_RS06975 at 184.849 kb on + strand, within EX31_RS06975 at 184.871 kb on + strand, within EX31_RS06975 at 184.871 kb on + strand, within EX31_RS06975 at 184.872 kb on - strand, within EX31_RS06975 at 184.872 kb on - strand, within EX31_RS06975
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 181,764 - EX31_RS06965 0.49 +0.3 181,812 - EX31_RS06965 0.53 +0.9 182,096 + EX31_RS06965 0.73 -0.3 182,159 + EX31_RS06965 0.77 +1.3 182,159 + EX31_RS06965 0.77 +0.9 182,160 - EX31_RS06965 0.77 -0.3 182,160 - EX31_RS06965 0.77 -2.1 182,298 - EX31_RS06965 0.87 +0.8 182,486 + +1.1 182,495 - -1.9 182,498 - -0.0 182,498 - +0.9 182,498 - +0.8 182,506 - +0.8 182,526 + +0.2 182,526 + +0.0 182,574 + -0.9 182,574 + +0.7 182,574 + -0.9 182,575 - +1.3 182,575 - +0.4 182,577 - +0.6 182,577 - -0.5 182,584 + -0.8 182,584 + +2.0 182,584 + +1.1 182,585 - +1.1 182,585 - -0.6 182,585 - +1.7 182,585 - +0.4 182,585 - -1.2 182,585 - +0.7 182,585 - +0.4 182,585 - -0.3 182,586 + +0.8 182,586 + -1.3 182,586 + -1.1 182,587 - -1.0 182,587 - +0.5 182,587 - +1.5 182,597 + -1.6 182,599 + +1.1 182,599 + -0.6 182,600 - +0.1 182,600 - -0.0 182,663 + +0.7 182,663 + -1.9 182,688 + +0.1 182,689 - -1.5 182,698 + -1.2 182,698 + -0.3 182,706 - -1.8 182,735 + +0.1 182,787 + +0.6 182,795 + -0.3 182,853 - EX31_RS06970 0.11 -0.2 182,905 + EX31_RS06970 0.16 -0.9 182,905 + EX31_RS06970 0.16 -0.4 182,906 - EX31_RS06970 0.16 -1.9 183,097 - EX31_RS06970 0.32 -1.1 183,302 + EX31_RS06970 0.49 +0.2 183,302 + EX31_RS06970 0.49 -1.5 183,302 + EX31_RS06970 0.49 -0.8 183,378 - EX31_RS06970 0.56 -1.8 183,433 + EX31_RS06970 0.60 -2.1 183,434 - EX31_RS06970 0.60 +0.7 183,434 - EX31_RS06970 0.60 -0.4 183,434 - EX31_RS06970 0.60 -1.5 183,489 + EX31_RS06970 0.65 +0.5 183,562 - EX31_RS06970 0.71 -2.2 183,755 + EX31_RS06970 0.87 -0.0 183,756 - EX31_RS06970 0.88 +0.7 183,965 + +1.2 183,966 - +1.2 183,979 + -0.3 184,036 + -0.3 184,036 + +1.2 184,036 + +0.2 184,071 + -0.1 184,132 + +0.3 184,132 + +0.4 184,132 + +0.2 184,535 + EX31_RS06975 0.32 +0.6 184,723 + EX31_RS06975 0.44 +1.0 184,723 + EX31_RS06975 0.44 +0.1 184,810 + EX31_RS06975 0.50 -0.2 184,849 + EX31_RS06975 0.53 -1.0 184,849 + EX31_RS06975 0.53 +1.4 184,871 + EX31_RS06975 0.54 -0.9 184,871 + EX31_RS06975 0.54 +0.3 184,872 - EX31_RS06975 0.54 +2.5 184,872 - EX31_RS06975 0.54 -0.5
Or see this region's nucleotide sequence