Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS09285 and EX31_RS09290 are separated by 14 nucleotides EX31_RS09290 and EX31_RS09295 are separated by 266 nucleotides EX31_RS09295 and EX31_RS09300 are separated by 168 nucleotides EX31_RS09300 and EX31_RS09305 are separated by 96 nucleotides
EX31_RS09285: EX31_RS09285 - hypothetical protein, at 58,624 to 58,929
_RS09285
EX31_RS09290: EX31_RS09290 - hypothetical protein, at 58,944 to 59,468
_RS09290
EX31_RS09295: EX31_RS09295 - D-amino-acid transaminase, at 59,735 to 60,598
_RS09295
EX31_RS09300: EX31_RS09300 - hypothetical protein, at 60,767 to 61,006
_RS09300
EX31_RS09305: EX31_RS09305 - respiratory nitrate reductase subunit gamma, at 61,103 to 61,783
_RS09305
Position (kb) 59
60
61 Strain fitness (log2 ratio) -2
-1
0
1 at 58.820 kb on - strand, within EX31_RS09285 at 58.987 kb on + strand at 59.042 kb on - strand, within EX31_RS09290 at 59.042 kb on - strand, within EX31_RS09290 at 59.099 kb on + strand, within EX31_RS09290 at 59.142 kb on - strand, within EX31_RS09290 at 59.376 kb on - strand, within EX31_RS09290 at 59.393 kb on - strand, within EX31_RS09290 at 59.432 kb on + strand at 59.432 kb on + strand at 59.505 kb on + strand at 59.606 kb on - strand at 59.732 kb on + strand at 59.733 kb on - strand at 59.737 kb on - strand at 59.769 kb on + strand at 59.932 kb on + strand, within EX31_RS09295 at 60.029 kb on + strand, within EX31_RS09295 at 60.030 kb on - strand, within EX31_RS09295 at 60.030 kb on - strand, within EX31_RS09295 at 60.083 kb on + strand, within EX31_RS09295 at 60.101 kb on - strand, within EX31_RS09295 at 60.115 kb on - strand, within EX31_RS09295 at 60.115 kb on - strand, within EX31_RS09295 at 60.227 kb on + strand, within EX31_RS09295 at 60.228 kb on - strand, within EX31_RS09295 at 60.228 kb on - strand, within EX31_RS09295 at 60.330 kb on + strand, within EX31_RS09295 at 60.330 kb on + strand, within EX31_RS09295 at 60.332 kb on + strand, within EX31_RS09295 at 60.332 kb on + strand, within EX31_RS09295 at 60.332 kb on + strand, within EX31_RS09295 at 60.332 kb on + strand, within EX31_RS09295 at 60.332 kb on + strand, within EX31_RS09295 at 60.333 kb on - strand, within EX31_RS09295 at 60.501 kb on + strand, within EX31_RS09295 at 60.501 kb on + strand, within EX31_RS09295 at 60.583 kb on - strand at 60.583 kb on - strand at 60.584 kb on + strand at 60.585 kb on - strand at 60.602 kb on - strand at 60.633 kb on + strand at 60.636 kb on + strand at 60.636 kb on + strand at 60.637 kb on - strand at 60.643 kb on - strand at 60.803 kb on + strand, within EX31_RS09300 at 60.985 kb on + strand at 60.985 kb on + strand at 60.986 kb on - strand at 61.048 kb on - strand at 61.067 kb on - strand at 61.067 kb on - strand at 61.128 kb on + strand at 61.143 kb on + strand at 61.143 kb on + strand at 61.331 kb on - strand, within EX31_RS09305 at 61.354 kb on + strand, within EX31_RS09305 at 61.551 kb on + strand, within EX31_RS09305 at 61.551 kb on + strand, within EX31_RS09305 at 61.551 kb on + strand, within EX31_RS09305 at 61.551 kb on + strand, within EX31_RS09305 at 61.551 kb on + strand, within EX31_RS09305 at 61.552 kb on - strand, within EX31_RS09305 at 61.552 kb on - strand, within EX31_RS09305
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 58,820 - EX31_RS09285 0.64 +0.8 58,987 + -0.5 59,042 - EX31_RS09290 0.19 -1.1 59,042 - EX31_RS09290 0.19 -0.1 59,099 + EX31_RS09290 0.30 -0.2 59,142 - EX31_RS09290 0.38 -0.4 59,376 - EX31_RS09290 0.82 -0.3 59,393 - EX31_RS09290 0.86 -0.7 59,432 + -1.2 59,432 + -0.1 59,505 + +0.4 59,606 - -0.0 59,732 + -1.4 59,733 - -0.3 59,737 - +0.6 59,769 + -0.2 59,932 + EX31_RS09295 0.23 +1.4 60,029 + EX31_RS09295 0.34 -0.6 60,030 - EX31_RS09295 0.34 -0.1 60,030 - EX31_RS09295 0.34 +0.3 60,083 + EX31_RS09295 0.40 +0.7 60,101 - EX31_RS09295 0.42 +1.2 60,115 - EX31_RS09295 0.44 -0.3 60,115 - EX31_RS09295 0.44 +0.7 60,227 + EX31_RS09295 0.57 +0.7 60,228 - EX31_RS09295 0.57 -0.7 60,228 - EX31_RS09295 0.57 +1.1 60,330 + EX31_RS09295 0.69 +0.0 60,330 + EX31_RS09295 0.69 +0.9 60,332 + EX31_RS09295 0.69 -1.1 60,332 + EX31_RS09295 0.69 -0.8 60,332 + EX31_RS09295 0.69 -0.4 60,332 + EX31_RS09295 0.69 -0.1 60,332 + EX31_RS09295 0.69 -1.4 60,333 - EX31_RS09295 0.69 +0.1 60,501 + EX31_RS09295 0.89 -1.6 60,501 + EX31_RS09295 0.89 -2.5 60,583 - -0.0 60,583 - -1.2 60,584 + -0.8 60,585 - -0.0 60,602 - -0.1 60,633 + +0.6 60,636 + -1.5 60,636 + +0.8 60,637 - +1.0 60,643 - +0.0 60,803 + EX31_RS09300 0.15 +0.5 60,985 + +0.2 60,985 + +0.8 60,986 - +0.6 61,048 - -0.1 61,067 - -1.1 61,067 - +0.2 61,128 + +0.2 61,143 + +0.9 61,143 + -0.5 61,331 - EX31_RS09305 0.33 +0.1 61,354 + EX31_RS09305 0.37 -1.4 61,551 + EX31_RS09305 0.66 +0.9 61,551 + EX31_RS09305 0.66 +1.1 61,551 + EX31_RS09305 0.66 +0.3 61,551 + EX31_RS09305 0.66 -0.4 61,551 + EX31_RS09305 0.66 +0.2 61,552 - EX31_RS09305 0.66 -0.3 61,552 - EX31_RS09305 0.66 -1.0
Or see this region's nucleotide sequence