Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS02205 and EX31_RS02215 are separated by 579 nucleotides EX31_RS02215 and EX31_RS02220 overlap by 48 nucleotides EX31_RS02220 and EX31_RS02225 are separated by 504 nucleotides
EX31_RS02205: EX31_RS02205 - DUF2778 domain-containing protein, at 479,745 to 480,221
_RS02205
EX31_RS02215: EX31_RS02215 - isopentenyl-diphosphate Delta-isomerase, at 480,801 to 481,334
_RS02215
EX31_RS02220: EX31_RS02220 - adenylate kinase, at 481,287 to 481,823
_RS02220
EX31_RS02225: EX31_RS02225 - hypothetical protein, at 482,328 to 482,585
_RS02225
Position (kb) 480
481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 479.901 kb on + strand, within EX31_RS02205 at 479.901 kb on + strand, within EX31_RS02205 at 479.904 kb on + strand, within EX31_RS02205 at 479.904 kb on + strand, within EX31_RS02205 at 479.904 kb on + strand, within EX31_RS02205 at 479.904 kb on + strand, within EX31_RS02205 at 479.904 kb on + strand, within EX31_RS02205 at 479.905 kb on - strand, within EX31_RS02205 at 479.905 kb on - strand, within EX31_RS02205 at 479.905 kb on - strand, within EX31_RS02205 at 479.905 kb on - strand, within EX31_RS02205 at 479.905 kb on - strand, within EX31_RS02205 at 479.937 kb on + strand, within EX31_RS02205 at 479.938 kb on - strand, within EX31_RS02205 at 479.957 kb on + strand, within EX31_RS02205 at 479.957 kb on + strand, within EX31_RS02205 at 480.014 kb on + strand, within EX31_RS02205 at 480.014 kb on + strand, within EX31_RS02205 at 480.015 kb on - strand, within EX31_RS02205 at 480.117 kb on - strand, within EX31_RS02205 at 480.122 kb on + strand, within EX31_RS02205 at 480.122 kb on + strand, within EX31_RS02205 at 480.154 kb on + strand, within EX31_RS02205 at 480.155 kb on - strand, within EX31_RS02205 at 480.155 kb on - strand, within EX31_RS02205 at 480.216 kb on + strand at 480.216 kb on + strand at 480.219 kb on + strand at 480.236 kb on + strand at 480.236 kb on + strand at 480.236 kb on + strand at 480.236 kb on + strand at 480.236 kb on + strand at 480.237 kb on - strand at 480.237 kb on - strand at 480.237 kb on - strand at 480.238 kb on + strand at 480.238 kb on + strand at 480.238 kb on + strand at 480.238 kb on + strand at 480.238 kb on + strand at 480.238 kb on + strand at 480.239 kb on - strand at 480.276 kb on + strand at 480.280 kb on - strand at 480.280 kb on - strand at 480.280 kb on - strand at 480.280 kb on - strand at 480.280 kb on - strand at 480.300 kb on + strand at 480.300 kb on + strand at 480.300 kb on + strand at 480.351 kb on + strand at 480.351 kb on + strand at 480.379 kb on + strand at 480.461 kb on + strand at 480.492 kb on - strand at 480.498 kb on + strand at 480.498 kb on + strand at 480.543 kb on + strand at 480.543 kb on + strand at 480.543 kb on + strand at 480.544 kb on - strand at 480.544 kb on - strand at 480.545 kb on + strand at 480.545 kb on + strand at 480.545 kb on + strand at 480.545 kb on + strand at 480.545 kb on + strand at 480.545 kb on + strand at 480.546 kb on - strand at 480.553 kb on + strand at 480.553 kb on + strand at 480.556 kb on - strand at 480.570 kb on + strand at 480.570 kb on + strand at 480.596 kb on - strand at 480.596 kb on - strand at 480.626 kb on + strand at 480.642 kb on + strand at 480.642 kb on + strand at 480.643 kb on - strand at 480.664 kb on + strand at 480.665 kb on - strand at 480.765 kb on + strand at 480.765 kb on + strand at 480.765 kb on + strand at 480.765 kb on + strand at 480.766 kb on - strand at 480.766 kb on - strand at 480.766 kb on - strand at 480.774 kb on + strand at 480.774 kb on + strand at 480.775 kb on - strand at 480.800 kb on + strand at 480.820 kb on + strand at 480.820 kb on + strand at 480.821 kb on - strand at 480.889 kb on + strand, within EX31_RS02215 at 480.889 kb on + strand, within EX31_RS02215 at 480.889 kb on + strand, within EX31_RS02215 at 480.890 kb on - strand, within EX31_RS02215 at 480.890 kb on - strand, within EX31_RS02215 at 480.890 kb on - strand, within EX31_RS02215 at 480.890 kb on - strand, within EX31_RS02215 at 480.890 kb on - strand, within EX31_RS02215 at 480.909 kb on + strand, within EX31_RS02215 at 480.910 kb on - strand, within EX31_RS02215 at 480.910 kb on - strand, within EX31_RS02215 at 480.911 kb on + strand, within EX31_RS02215 at 480.911 kb on + strand, within EX31_RS02215 at 480.912 kb on - strand, within EX31_RS02215 at 480.912 kb on - strand, within EX31_RS02215 at 481.289 kb on - strand at 481.434 kb on - strand, within EX31_RS02220 at 481.434 kb on - strand, within EX31_RS02220 at 481.434 kb on - strand, within EX31_RS02220 at 481.434 kb on - strand, within EX31_RS02220 at 481.544 kb on - strand, within EX31_RS02220 at 481.544 kb on - strand, within EX31_RS02220 at 481.545 kb on + strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.546 kb on - strand, within EX31_RS02220 at 481.550 kb on + strand, within EX31_RS02220 at 481.551 kb on - strand, within EX31_RS02220 at 481.564 kb on + strand, within EX31_RS02220 at 481.584 kb on - strand, within EX31_RS02220 at 481.613 kb on + strand, within EX31_RS02220 at 481.622 kb on - strand, within EX31_RS02220 at 481.739 kb on + strand, within EX31_RS02220 at 481.739 kb on + strand, within EX31_RS02220 at 481.739 kb on + strand, within EX31_RS02220 at 481.739 kb on + strand, within EX31_RS02220 at 481.739 kb on + strand, within EX31_RS02220 at 481.740 kb on - strand, within EX31_RS02220 at 481.740 kb on - strand, within EX31_RS02220 at 481.742 kb on - strand, within EX31_RS02220 at 481.742 kb on - strand, within EX31_RS02220 at 481.742 kb on - strand, within EX31_RS02220 at 481.749 kb on - strand, within EX31_RS02220 at 481.749 kb on - strand, within EX31_RS02220 at 481.880 kb on + strand at 481.881 kb on - strand at 481.881 kb on - strand at 481.884 kb on - strand at 481.884 kb on - strand at 481.895 kb on + strand at 481.895 kb on + strand at 481.896 kb on - strand at 481.896 kb on - strand at 481.906 kb on + strand at 481.907 kb on - strand at 481.907 kb on - strand at 481.922 kb on - strand at 481.937 kb on + strand at 481.949 kb on + strand at 481.949 kb on + strand at 481.949 kb on + strand at 481.949 kb on + strand at 481.949 kb on + strand at 481.949 kb on + strand at 481.950 kb on - strand at 481.967 kb on + strand at 481.968 kb on - strand at 481.968 kb on - strand at 481.968 kb on - strand at 481.968 kb on - strand at 481.971 kb on - strand at 482.033 kb on - strand at 482.045 kb on + strand at 482.045 kb on + strand at 482.046 kb on - strand at 482.046 kb on - strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.100 kb on + strand at 482.101 kb on - strand at 482.101 kb on - strand at 482.101 kb on - strand at 482.112 kb on + strand at 482.201 kb on + strand at 482.202 kb on - strand at 482.202 kb on - strand at 482.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 479,901 + EX31_RS02205 0.33 -0.3 479,901 + EX31_RS02205 0.33 -0.9 479,904 + EX31_RS02205 0.33 -2.4 479,904 + EX31_RS02205 0.33 +0.8 479,904 + EX31_RS02205 0.33 +0.8 479,904 + EX31_RS02205 0.33 +0.5 479,904 + EX31_RS02205 0.33 +0.5 479,905 - EX31_RS02205 0.34 +0.8 479,905 - EX31_RS02205 0.34 -2.6 479,905 - EX31_RS02205 0.34 +0.2 479,905 - EX31_RS02205 0.34 +1.2 479,905 - EX31_RS02205 0.34 +0.8 479,937 + EX31_RS02205 0.40 +0.7 479,938 - EX31_RS02205 0.40 +0.7 479,957 + EX31_RS02205 0.44 -1.2 479,957 + EX31_RS02205 0.44 -1.0 480,014 + EX31_RS02205 0.56 -1.9 480,014 + EX31_RS02205 0.56 -2.3 480,015 - EX31_RS02205 0.57 -0.8 480,117 - EX31_RS02205 0.78 -1.5 480,122 + EX31_RS02205 0.79 +0.5 480,122 + EX31_RS02205 0.79 +0.8 480,154 + EX31_RS02205 0.86 -0.5 480,155 - EX31_RS02205 0.86 -0.1 480,155 - EX31_RS02205 0.86 -1.3 480,216 + +2.9 480,216 + -1.7 480,219 + +0.1 480,236 + -0.8 480,236 + +0.6 480,236 + +0.0 480,236 + -1.0 480,236 + +0.3 480,237 - -1.4 480,237 - -1.1 480,237 - -1.5 480,238 + -0.1 480,238 + -0.6 480,238 + -3.1 480,238 + -1.5 480,238 + -0.1 480,238 + -0.3 480,239 - +0.3 480,276 + -0.4 480,280 - +0.2 480,280 - -1.8 480,280 - +1.3 480,280 - -1.0 480,280 - +0.1 480,300 + +0.2 480,300 + +1.2 480,300 + +0.2 480,351 + -0.2 480,351 + +1.0 480,379 + +0.1 480,461 + -1.5 480,492 - -0.4 480,498 + -0.8 480,498 + +0.3 480,543 + +0.7 480,543 + -0.4 480,543 + +0.3 480,544 - +0.7 480,544 - +0.1 480,545 + +0.1 480,545 + -0.5 480,545 + -0.6 480,545 + -2.0 480,545 + -0.6 480,545 + -0.5 480,546 - -0.2 480,553 + +1.1 480,553 + -0.6 480,556 - -0.5 480,570 + +0.6 480,570 + -0.6 480,596 - -1.4 480,596 - +0.0 480,626 + -1.0 480,642 + -0.8 480,642 + +0.1 480,643 - -1.1 480,664 + +1.6 480,665 - -2.0 480,765 + -0.8 480,765 + +0.6 480,765 + +0.7 480,765 + -1.7 480,766 - +0.4 480,766 - +0.2 480,766 - -0.9 480,774 + +1.1 480,774 + -1.5 480,775 - -1.1 480,800 + +1.1 480,820 + +0.0 480,820 + -0.9 480,821 - -0.2 480,889 + EX31_RS02215 0.16 +0.7 480,889 + EX31_RS02215 0.16 -0.8 480,889 + EX31_RS02215 0.16 -0.9 480,890 - EX31_RS02215 0.17 -1.3 480,890 - EX31_RS02215 0.17 -1.2 480,890 - EX31_RS02215 0.17 +0.3 480,890 - EX31_RS02215 0.17 -0.2 480,890 - EX31_RS02215 0.17 -0.8 480,909 + EX31_RS02215 0.20 +1.3 480,910 - EX31_RS02215 0.20 -0.4 480,910 - EX31_RS02215 0.20 -1.2 480,911 + EX31_RS02215 0.21 -3.9 480,911 + EX31_RS02215 0.21 +0.5 480,912 - EX31_RS02215 0.21 -1.2 480,912 - EX31_RS02215 0.21 -0.9 481,289 - +1.1 481,434 - EX31_RS02220 0.27 -1.5 481,434 - EX31_RS02220 0.27 -2.9 481,434 - EX31_RS02220 0.27 -0.1 481,434 - EX31_RS02220 0.27 +0.1 481,544 - EX31_RS02220 0.48 -0.4 481,544 - EX31_RS02220 0.48 -1.1 481,545 + EX31_RS02220 0.48 -0.7 481,546 - EX31_RS02220 0.48 -0.5 481,546 - EX31_RS02220 0.48 +1.0 481,546 - EX31_RS02220 0.48 +0.2 481,546 - EX31_RS02220 0.48 -0.5 481,546 - EX31_RS02220 0.48 -2.9 481,546 - EX31_RS02220 0.48 -0.2 481,546 - EX31_RS02220 0.48 -0.0 481,550 + EX31_RS02220 0.49 -0.7 481,551 - EX31_RS02220 0.49 -1.8 481,564 + EX31_RS02220 0.52 -0.1 481,584 - EX31_RS02220 0.55 +0.5 481,613 + EX31_RS02220 0.61 +0.6 481,622 - EX31_RS02220 0.62 -0.9 481,739 + EX31_RS02220 0.84 -0.8 481,739 + EX31_RS02220 0.84 -0.3 481,739 + EX31_RS02220 0.84 -0.1 481,739 + EX31_RS02220 0.84 -0.8 481,739 + EX31_RS02220 0.84 -0.5 481,740 - EX31_RS02220 0.84 +1.8 481,740 - EX31_RS02220 0.84 +0.7 481,742 - EX31_RS02220 0.85 -1.4 481,742 - EX31_RS02220 0.85 -0.3 481,742 - EX31_RS02220 0.85 -0.7 481,749 - EX31_RS02220 0.86 -0.3 481,749 - EX31_RS02220 0.86 +0.2 481,880 + -0.1 481,881 - -2.8 481,881 - -0.4 481,884 - -0.4 481,884 - -0.5 481,895 + +0.3 481,895 + -1.4 481,896 - -0.0 481,896 - -0.2 481,906 + +0.1 481,907 - -0.2 481,907 - -0.1 481,922 - +0.4 481,937 + -0.1 481,949 + +0.6 481,949 + +1.6 481,949 + -2.0 481,949 + -0.6 481,949 + -0.1 481,949 + +0.1 481,950 - -0.8 481,967 + -0.1 481,968 - +1.1 481,968 - +0.4 481,968 - +0.1 481,968 - -1.7 481,971 - -0.9 482,033 - +0.5 482,045 + -0.9 482,045 + -1.6 482,046 - -2.2 482,046 - -0.4 482,100 + -0.2 482,100 + +0.8 482,100 + -0.4 482,100 + +0.2 482,100 + +0.2 482,100 + +0.7 482,100 + +0.6 482,100 + +0.2 482,101 - +0.1 482,101 - -0.9 482,101 - +0.9 482,112 + -0.9 482,201 + +0.1 482,202 - -1.2 482,202 - -0.3 482,255 + +1.3
Or see this region's nucleotide sequence