Strain Fitness in Rahnella sp. WP5 around EX31_RS05995

Experiment: MGL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX31_RS25170 and EX31_RS25175 are separated by 18 nucleotidesEX31_RS25175 and EX31_RS25180 overlap by 37 nucleotidesEX31_RS25180 and EX31_RS05995 are separated by 2 nucleotidesEX31_RS05995 and EX31_RS06000 are separated by 112 nucleotides EX31_RS25170: EX31_RS25170 - 50S ribosomal protein L34, at 357,537 to 357,677 _RS25170 EX31_RS25175: EX31_RS25175 - ribonuclease P protein component, at 357,696 to 358,055 _RS25175 EX31_RS25180: EX31_RS25180 - membrane protein insertion efficiency factor YidD, at 358,019 to 358,276 _RS25180 EX31_RS05995: EX31_RS05995 - membrane protein insertase YidC, at 358,279 to 359,913 _RS05995 EX31_RS06000: EX31_RS06000 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 360,026 to 361,390 _RS06000 Position (kb) 358 359 360Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 357.289 kb on + strandat 357.289 kb on + strandat 357.290 kb on - strandat 357.305 kb on - strandat 357.305 kb on - strandat 357.305 kb on - strandat 357.305 kb on - strandat 357.335 kb on - strandat 357.346 kb on + strandat 358.018 kb on + strand, within EX31_RS25175at 358.080 kb on + strand, within EX31_RS25180at 358.080 kb on + strand, within EX31_RS25180at 358.136 kb on + strand, within EX31_RS25180at 358.136 kb on + strand, within EX31_RS25180at 358.136 kb on + strand, within EX31_RS25180at 358.136 kb on + strand, within EX31_RS25180at 358.141 kb on + strand, within EX31_RS25180at 358.141 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.170 kb on + strand, within EX31_RS25180at 358.203 kb on + strand, within EX31_RS25180at 358.203 kb on + strand, within EX31_RS25180at 358.203 kb on + strand, within EX31_RS25180at 359.860 kb on + strandat 359.860 kb on + strandat 359.911 kb on + strandat 359.911 kb on + strandat 359.911 kb on + strandat 359.912 kb on - strandat 359.958 kb on + strandat 359.959 kb on - strandat 359.994 kb on + strandat 360.000 kb on + strandat 360.001 kb on - strandat 360.025 kb on + strandat 360.025 kb on + strandat 360.026 kb on - strandat 360.026 kb on - strandat 360.046 kb on + strandat 360.135 kb on + strandat 360.139 kb on + strandat 360.140 kb on - strandat 360.158 kb on + strandat 360.168 kb on - strand, within EX31_RS06000at 360.168 kb on - strand, within EX31_RS06000at 360.168 kb on - strand, within EX31_RS06000at 360.168 kb on - strand, within EX31_RS06000at 360.317 kb on - strand, within EX31_RS06000at 360.382 kb on + strand, within EX31_RS06000at 360.592 kb on - strand, within EX31_RS06000at 360.607 kb on + strand, within EX31_RS06000at 360.799 kb on + strand, within EX31_RS06000at 360.799 kb on + strand, within EX31_RS06000at 360.800 kb on - strand, within EX31_RS06000

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Per-strain Table

Position Strand Gene LocusTag Fraction MGL
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357,289 + +0.7
357,289 + +0.3
357,290 - -0.6
357,305 - +0.4
357,305 - -0.2
357,305 - +0.3
357,305 - -0.4
357,335 - -0.3
357,346 + -0.6
358,018 + EX31_RS25175 0.89 +0.2
358,080 + EX31_RS25180 0.24 +0.3
358,080 + EX31_RS25180 0.24 +0.8
358,136 + EX31_RS25180 0.45 -0.6
358,136 + EX31_RS25180 0.45 -2.0
358,136 + EX31_RS25180 0.45 -0.6
358,136 + EX31_RS25180 0.45 +0.1
358,141 + EX31_RS25180 0.47 -0.1
358,141 + EX31_RS25180 0.47 -1.3
358,170 + EX31_RS25180 0.59 +1.1
358,170 + EX31_RS25180 0.59 +1.0
358,170 + EX31_RS25180 0.59 +1.2
358,170 + EX31_RS25180 0.59 -0.1
358,170 + EX31_RS25180 0.59 +0.3
358,170 + EX31_RS25180 0.59 +0.6
358,203 + EX31_RS25180 0.71 -1.4
358,203 + EX31_RS25180 0.71 -0.8
358,203 + EX31_RS25180 0.71 -2.5
359,860 + +0.3
359,860 + -0.1
359,911 + -0.0
359,911 + +0.3
359,911 + +0.1
359,912 - +1.0
359,958 + +0.0
359,959 - -0.8
359,994 + -3.8
360,000 + -1.5
360,001 - -2.5
360,025 + +0.5
360,025 + -1.2
360,026 - -2.2
360,026 - -0.2
360,046 + -1.5
360,135 + -1.7
360,139 + -1.8
360,140 - -0.4
360,158 + -2.0
360,168 - EX31_RS06000 0.10 -1.0
360,168 - EX31_RS06000 0.10 -0.9
360,168 - EX31_RS06000 0.10 -0.5
360,168 - EX31_RS06000 0.10 +0.2
360,317 - EX31_RS06000 0.21 -1.5
360,382 + EX31_RS06000 0.26 -0.8
360,592 - EX31_RS06000 0.41 -0.4
360,607 + EX31_RS06000 0.43 -1.3
360,799 + EX31_RS06000 0.57 -1.5
360,799 + EX31_RS06000 0.57 -0.0
360,800 - EX31_RS06000 0.57 +0.9

Or see this region's nucleotide sequence