Experiment: MGL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX31_RS25170 and EX31_RS25175 are separated by 18 nucleotides EX31_RS25175 and EX31_RS25180 overlap by 37 nucleotides EX31_RS25180 and EX31_RS05995 are separated by 2 nucleotides EX31_RS05995 and EX31_RS06000 are separated by 112 nucleotides
EX31_RS25170: EX31_RS25170 - 50S ribosomal protein L34, at 357,537 to 357,677
_RS25170
EX31_RS25175: EX31_RS25175 - ribonuclease P protein component, at 357,696 to 358,055
_RS25175
EX31_RS25180: EX31_RS25180 - membrane protein insertion efficiency factor YidD, at 358,019 to 358,276
_RS25180
EX31_RS05995: EX31_RS05995 - membrane protein insertase YidC, at 358,279 to 359,913
_RS05995
EX31_RS06000: EX31_RS06000 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 360,026 to 361,390
_RS06000
Position (kb) 358
359
360 Strain fitness (log2 ratio) -2
-1
0
1 at 357.289 kb on + strand at 357.289 kb on + strand at 357.290 kb on - strand at 357.305 kb on - strand at 357.305 kb on - strand at 357.305 kb on - strand at 357.305 kb on - strand at 357.335 kb on - strand at 357.346 kb on + strand at 358.018 kb on + strand, within EX31_RS25175 at 358.080 kb on + strand, within EX31_RS25180 at 358.080 kb on + strand, within EX31_RS25180 at 358.136 kb on + strand, within EX31_RS25180 at 358.136 kb on + strand, within EX31_RS25180 at 358.136 kb on + strand, within EX31_RS25180 at 358.136 kb on + strand, within EX31_RS25180 at 358.141 kb on + strand, within EX31_RS25180 at 358.141 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.170 kb on + strand, within EX31_RS25180 at 358.203 kb on + strand, within EX31_RS25180 at 358.203 kb on + strand, within EX31_RS25180 at 358.203 kb on + strand, within EX31_RS25180 at 359.860 kb on + strand at 359.860 kb on + strand at 359.911 kb on + strand at 359.911 kb on + strand at 359.911 kb on + strand at 359.912 kb on - strand at 359.958 kb on + strand at 359.959 kb on - strand at 359.994 kb on + strand at 360.000 kb on + strand at 360.001 kb on - strand at 360.025 kb on + strand at 360.025 kb on + strand at 360.026 kb on - strand at 360.026 kb on - strand at 360.046 kb on + strand at 360.135 kb on + strand at 360.139 kb on + strand at 360.140 kb on - strand at 360.158 kb on + strand at 360.168 kb on - strand, within EX31_RS06000 at 360.168 kb on - strand, within EX31_RS06000 at 360.168 kb on - strand, within EX31_RS06000 at 360.168 kb on - strand, within EX31_RS06000 at 360.317 kb on - strand, within EX31_RS06000 at 360.382 kb on + strand, within EX31_RS06000 at 360.592 kb on - strand, within EX31_RS06000 at 360.607 kb on + strand, within EX31_RS06000 at 360.799 kb on + strand, within EX31_RS06000 at 360.799 kb on + strand, within EX31_RS06000 at 360.800 kb on - strand, within EX31_RS06000
Per-strain Table
Position Strand Gene LocusTag Fraction MGL remove 357,289 + +0.6 357,289 + -0.2 357,290 - -0.2 357,305 - +0.3 357,305 - +0.1 357,305 - -0.6 357,305 - +0.2 357,335 - +0.3 357,346 + -0.5 358,018 + EX31_RS25175 0.89 +0.0 358,080 + EX31_RS25180 0.24 -0.4 358,080 + EX31_RS25180 0.24 -0.8 358,136 + EX31_RS25180 0.45 -0.4 358,136 + EX31_RS25180 0.45 -1.2 358,136 + EX31_RS25180 0.45 -1.6 358,136 + EX31_RS25180 0.45 +0.9 358,141 + EX31_RS25180 0.47 +1.2 358,141 + EX31_RS25180 0.47 +0.7 358,170 + EX31_RS25180 0.59 -0.1 358,170 + EX31_RS25180 0.59 +1.1 358,170 + EX31_RS25180 0.59 +0.8 358,170 + EX31_RS25180 0.59 -0.3 358,170 + EX31_RS25180 0.59 -0.4 358,170 + EX31_RS25180 0.59 +0.5 358,203 + EX31_RS25180 0.71 +0.2 358,203 + EX31_RS25180 0.71 +0.6 358,203 + EX31_RS25180 0.71 -0.3 359,860 + +1.3 359,860 + +0.3 359,911 + -0.1 359,911 + +0.0 359,911 + -0.2 359,912 - +0.8 359,958 + -0.7 359,959 - +0.0 359,994 + +0.3 360,000 + +0.1 360,001 - -1.7 360,025 + +0.3 360,025 + -1.0 360,026 - -2.4 360,026 - -2.6 360,046 + +0.4 360,135 + -0.3 360,139 + -2.0 360,140 - -0.6 360,158 + -0.2 360,168 - EX31_RS06000 0.10 +0.8 360,168 - EX31_RS06000 0.10 -1.1 360,168 - EX31_RS06000 0.10 -2.3 360,168 - EX31_RS06000 0.10 +0.0 360,317 - EX31_RS06000 0.21 +0.6 360,382 + EX31_RS06000 0.26 -1.0 360,592 - EX31_RS06000 0.41 -0.6 360,607 + EX31_RS06000 0.43 -1.0 360,799 + EX31_RS06000 0.57 -1.7 360,799 + EX31_RS06000 0.57 -0.2 360,800 - EX31_RS06000 0.57 -0.3
Or see this region's nucleotide sequence