Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5616

Experiment: Putrescine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5614 and Pf1N1B4_5615 overlap by 4 nucleotidesPf1N1B4_5615 and Pf1N1B4_5616 are separated by 61 nucleotidesPf1N1B4_5616 and Pf1N1B4_6052 are separated by 168 nucleotidesPf1N1B4_6052 and Pf1N1B4_5617 are separated by 63 nucleotidesPf1N1B4_5617 and Pf1N1B4_5618 are separated by 33 nucleotides Pf1N1B4_5614 - COGs COG3777, at 40,025 to 40,867 _5614 Pf1N1B4_5615 - Citrate lyase beta chain (EC 4.1.3.6), at 40,864 to 41,679 _5615 Pf1N1B4_5616 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 41,741 to 43,132 _5616 Pf1N1B4_6052 - tRNA-Gly-GCC, at 43,301 to 43,373 _6052 Pf1N1B4_5617 - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (EC 2.7.8.5), at 43,437 to 43,997 _5617 Pf1N1B4_5618 - Excinuclease ABC subunit C, at 44,031 to 45,854 _5618 Position (kb) 41 42 43 44Strain fitness (log2 ratio) -1 0 1 2at 41.433 kb on + strand, within Pf1N1B4_5615at 41.682 kb on - strandat 41.807 kb on + strandat 41.931 kb on + strand, within Pf1N1B4_5616at 42.212 kb on + strand, within Pf1N1B4_5616at 42.212 kb on + strand, within Pf1N1B4_5616at 42.212 kb on + strand, within Pf1N1B4_5616at 42.213 kb on - strand, within Pf1N1B4_5616at 42.213 kb on - strand, within Pf1N1B4_5616at 42.251 kb on + strand, within Pf1N1B4_5616at 42.659 kb on + strand, within Pf1N1B4_5616at 42.659 kb on + strandat 42.659 kb on + strand, within Pf1N1B4_5616at 42.660 kb on - strand, within Pf1N1B4_5616at 42.660 kb on - strand, within Pf1N1B4_5616at 42.687 kb on + strand, within Pf1N1B4_5616at 42.936 kb on - strand, within Pf1N1B4_5616at 43.439 kb on - strandat 44.033 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Putrescine (N)
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41,433 + Pf1N1B4_5615 0.70 +0.1
41,682 - -0.3
41,807 + +0.7
41,931 + Pf1N1B4_5616 0.14 +1.2
42,212 + Pf1N1B4_5616 0.34 +0.9
42,212 + Pf1N1B4_5616 0.34 -0.3
42,212 + Pf1N1B4_5616 0.34 -0.3
42,213 - Pf1N1B4_5616 0.34 -0.7
42,213 - Pf1N1B4_5616 0.34 +2.0
42,251 + Pf1N1B4_5616 0.37 -1.3
42,659 + Pf1N1B4_5616 0.66 -1.7
42,659 + +2.0
42,659 + Pf1N1B4_5616 0.66 -0.1
42,660 - Pf1N1B4_5616 0.66 +0.4
42,660 - Pf1N1B4_5616 0.66 +0.1
42,687 + Pf1N1B4_5616 0.68 -1.3
42,936 - Pf1N1B4_5616 0.86 -0.8
43,439 - +2.0
44,033 - +1.1

Or see this region's nucleotide sequence