Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotides Pf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides Pf1N1B4_5502 and Pf1N1B4_5503 are separated by 499 nucleotides
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413
_5501
Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542
_5502
Pf1N1B4_5503 - hypothetical protein, at 253,042 to 253,266
_5503
Position (kb) 251
252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.737 kb on - strand, within Pf1N1B4_5500 at 250.757 kb on - strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.771 kb on - strand at 250.805 kb on - strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.870 kb on + strand at 250.870 kb on + strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.977 kb on - strand at 250.977 kb on - strand at 251.024 kb on + strand at 251.024 kb on + strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.068 kb on - strand at 251.260 kb on + strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.279 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.411 kb on + strand at 251.411 kb on + strand at 251.412 kb on - strand at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.534 kb on + strand, within Pf1N1B4_5502 at 251.590 kb on + strand at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.740 kb on - strand, within Pf1N1B4_5502 at 251.798 kb on + strand, within Pf1N1B4_5502 at 251.994 kb on + strand, within Pf1N1B4_5502 at 251.995 kb on - strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.043 kb on + strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.044 kb on - strand, within Pf1N1B4_5502 at 252.115 kb on - strand, within Pf1N1B4_5502 at 252.143 kb on + strand, within Pf1N1B4_5502 at 252.144 kb on - strand, within Pf1N1B4_5502 at 252.144 kb on - strand at 252.144 kb on - strand, within Pf1N1B4_5502 at 252.179 kb on + strand, within Pf1N1B4_5502 at 252.180 kb on - strand, within Pf1N1B4_5502 at 252.185 kb on + strand, within Pf1N1B4_5502 at 252.239 kb on + strand, within Pf1N1B4_5502 at 252.274 kb on + strand, within Pf1N1B4_5502 at 252.275 kb on - strand, within Pf1N1B4_5502 at 252.382 kb on + strand, within Pf1N1B4_5502 at 252.383 kb on - strand, within Pf1N1B4_5502 at 252.419 kb on + strand, within Pf1N1B4_5502 at 252.420 kb on - strand, within Pf1N1B4_5502 at 252.420 kb on - strand, within Pf1N1B4_5502 at 252.429 kb on + strand at 252.429 kb on + strand at 252.429 kb on + strand at 252.430 kb on - strand at 252.430 kb on - strand at 252.435 kb on + strand at 252.435 kb on + strand at 252.435 kb on + strand at 252.436 kb on - strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.553 kb on + strand at 252.554 kb on - strand at 252.554 kb on - strand at 252.589 kb on + strand at 252.589 kb on + strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.590 kb on - strand at 252.652 kb on - strand at 252.661 kb on + strand at 252.732 kb on + strand at 252.732 kb on + strand at 252.732 kb on + strand at 252.807 kb on - strand at 252.821 kb on + strand at 252.836 kb on - strand at 252.902 kb on + strand at 252.936 kb on + strand at 252.936 kb on + strand at 252.936 kb on + strand at 252.944 kb on - strand at 252.946 kb on + strand at 252.993 kb on + strand at 252.993 kb on + strand at 252.994 kb on - strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.995 kb on + strand at 252.996 kb on - strand at 252.996 kb on - strand at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.123 kb on + strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.124 kb on - strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.129 kb on + strand, within Pf1N1B4_5503 at 253.130 kb on - strand, within Pf1N1B4_5503 at 253.130 kb on - strand, within Pf1N1B4_5503 at 253.267 kb on - strand at 253.315 kb on - strand at 253.315 kb on - strand at 253.380 kb on - strand at 253.380 kb on - strand at 253.426 kb on + strand at 253.427 kb on - strand at 253.427 kb on - strand at 253.441 kb on - strand at 253.473 kb on + strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.474 kb on - strand at 253.477 kb on - strand at 253.477 kb on - strand at 253.492 kb on - strand at 253.494 kb on + strand at 253.495 kb on - strand at 253.518 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 250,429 + Pf1N1B4_5500 0.56 -2.5 250,429 + Pf1N1B4_5500 0.56 +0.0 250,430 - Pf1N1B4_5500 0.56 -1.8 250,430 - Pf1N1B4_5500 0.56 +1.3 250,430 - Pf1N1B4_5500 0.56 +2.4 250,430 - Pf1N1B4_5500 0.56 -0.8 250,689 - Pf1N1B4_5500 0.82 -0.4 250,689 - Pf1N1B4_5500 0.82 -0.4 250,737 - Pf1N1B4_5500 0.87 +1.0 250,757 - +1.6 250,758 + Pf1N1B4_5500 0.89 +1.8 250,758 + -1.5 250,758 + Pf1N1B4_5500 0.89 +0.0 250,758 + Pf1N1B4_5500 0.89 +0.0 250,759 - Pf1N1B4_5500 0.89 -2.5 250,759 - Pf1N1B4_5500 0.89 +0.0 250,759 - Pf1N1B4_5500 0.89 +0.8 250,759 - Pf1N1B4_5500 0.89 +0.3 250,759 - Pf1N1B4_5500 0.89 -1.0 250,759 - Pf1N1B4_5500 0.89 -0.3 250,759 - Pf1N1B4_5500 0.89 +0.6 250,759 - Pf1N1B4_5500 0.89 -0.5 250,771 - +0.0 250,805 - -2.5 250,807 + -0.4 250,807 + +3.2 250,807 + +0.6 250,870 + -1.0 250,870 + +1.8 250,871 - +0.6 250,871 - -1.0 250,871 - -1.3 250,976 + +0.0 250,976 + -1.4 250,976 + -0.5 250,977 - -0.5 250,977 - -0.3 251,024 + +0.4 251,024 + -1.0 251,025 - -1.0 251,025 - -0.7 251,025 - -0.2 251,025 - -1.2 251,025 - +0.0 251,025 - +2.4 251,067 + +0.5 251,067 + -1.0 251,067 + +1.0 251,068 - +0.0 251,260 + Pf1N1B4_5501 0.55 +0.0 251,261 - Pf1N1B4_5501 0.55 -0.1 251,261 - Pf1N1B4_5501 0.55 +0.0 251,279 + Pf1N1B4_5501 0.61 -0.1 251,302 + Pf1N1B4_5501 0.67 -0.2 251,302 + Pf1N1B4_5501 0.67 -2.0 251,302 + Pf1N1B4_5501 0.67 +0.3 251,302 + Pf1N1B4_5501 0.67 +0.0 251,303 - Pf1N1B4_5501 0.68 -0.5 251,303 - Pf1N1B4_5501 0.68 -1.0 251,303 - Pf1N1B4_5501 0.68 +0.0 251,411 + +0.6 251,411 + -0.4 251,412 - -1.0 251,517 + Pf1N1B4_5502 0.12 +0.5 251,517 + Pf1N1B4_5502 0.12 +0.0 251,517 + Pf1N1B4_5502 0.12 +0.0 251,517 + Pf1N1B4_5502 0.12 -0.8 251,517 + Pf1N1B4_5502 0.12 -0.7 251,517 + Pf1N1B4_5502 0.12 +0.0 251,518 - Pf1N1B4_5502 0.12 +1.6 251,518 - Pf1N1B4_5502 0.12 +0.4 251,534 + Pf1N1B4_5502 0.13 +0.2 251,590 + -1.0 251,739 + Pf1N1B4_5502 0.31 -2.0 251,739 + Pf1N1B4_5502 0.31 -0.7 251,739 + Pf1N1B4_5502 0.31 -0.6 251,740 - Pf1N1B4_5502 0.31 +0.5 251,798 + Pf1N1B4_5502 0.36 -0.1 251,994 + Pf1N1B4_5502 0.53 +0.8 251,995 - Pf1N1B4_5502 0.53 -1.5 252,043 + Pf1N1B4_5502 0.57 -0.5 252,043 + Pf1N1B4_5502 0.57 +0.0 252,043 + Pf1N1B4_5502 0.57 +0.0 252,043 + Pf1N1B4_5502 0.57 +1.0 252,043 + Pf1N1B4_5502 0.57 -0.4 252,044 - Pf1N1B4_5502 0.57 +0.4 252,044 - Pf1N1B4_5502 0.57 -0.5 252,044 - -2.0 252,044 - Pf1N1B4_5502 0.57 -0.3 252,044 - Pf1N1B4_5502 0.57 +1.0 252,044 - Pf1N1B4_5502 0.57 +0.3 252,044 - Pf1N1B4_5502 0.57 -1.5 252,044 - Pf1N1B4_5502 0.57 -0.3 252,044 - Pf1N1B4_5502 0.57 +0.0 252,115 - Pf1N1B4_5502 0.63 -2.3 252,143 + Pf1N1B4_5502 0.66 +0.0 252,144 - Pf1N1B4_5502 0.66 -1.2 252,144 - +1.6 252,144 - Pf1N1B4_5502 0.66 +1.2 252,179 + Pf1N1B4_5502 0.69 -0.7 252,180 - Pf1N1B4_5502 0.69 +0.2 252,185 + Pf1N1B4_5502 0.69 +1.0 252,239 + Pf1N1B4_5502 0.74 +0.0 252,274 + Pf1N1B4_5502 0.77 +0.6 252,275 - Pf1N1B4_5502 0.77 -1.5 252,382 + Pf1N1B4_5502 0.86 -0.5 252,383 - Pf1N1B4_5502 0.86 +2.2 252,419 + Pf1N1B4_5502 0.89 -0.6 252,420 - Pf1N1B4_5502 0.89 -0.4 252,420 - Pf1N1B4_5502 0.89 +0.0 252,429 + +0.0 252,429 + -0.0 252,429 + +0.5 252,430 - -0.4 252,430 - +0.0 252,435 + +1.8 252,435 + -0.2 252,435 + -0.6 252,436 - +0.6 252,553 + -0.4 252,553 + -0.8 252,553 + +1.6 252,553 + -0.5 252,554 - +0.0 252,554 - -0.0 252,589 + -1.0 252,589 + +1.0 252,590 - -3.1 252,590 - -1.0 252,590 - -1.3 252,590 - -0.3 252,652 - +1.0 252,661 + -1.0 252,732 + +0.8 252,732 + +0.5 252,732 + +0.4 252,807 - -1.0 252,821 + +0.0 252,836 - -1.0 252,902 + +1.6 252,936 + +0.0 252,936 + -1.8 252,936 + +1.2 252,944 - -0.5 252,946 + -0.4 252,993 + -0.5 252,993 + -0.3 252,994 - -1.0 252,995 + -1.5 252,995 + +1.2 252,995 + +0.0 252,995 + +1.8 252,996 - -0.4 252,996 - -0.3 253,123 + Pf1N1B4_5503 0.36 -1.0 253,123 + Pf1N1B4_5503 0.36 +0.6 253,123 + Pf1N1B4_5503 0.36 -0.6 253,123 + Pf1N1B4_5503 0.36 +0.0 253,123 + Pf1N1B4_5503 0.36 +0.0 253,124 - Pf1N1B4_5503 0.36 -1.0 253,124 - Pf1N1B4_5503 0.36 -1.0 253,124 - Pf1N1B4_5503 0.36 -1.8 253,124 - Pf1N1B4_5503 0.36 -0.3 253,124 - Pf1N1B4_5503 0.36 +0.0 253,124 - Pf1N1B4_5503 0.36 +0.0 253,124 - Pf1N1B4_5503 0.36 +0.6 253,124 - Pf1N1B4_5503 0.36 -1.0 253,124 - Pf1N1B4_5503 0.36 +0.0 253,129 + Pf1N1B4_5503 0.39 -0.5 253,129 + Pf1N1B4_5503 0.39 +0.3 253,129 + Pf1N1B4_5503 0.39 +0.0 253,129 + Pf1N1B4_5503 0.39 -0.5 253,129 + Pf1N1B4_5503 0.39 -0.1 253,130 - Pf1N1B4_5503 0.39 -0.7 253,130 - Pf1N1B4_5503 0.39 +0.0 253,267 - -0.5 253,315 - -1.7 253,315 - -0.8 253,380 - +0.8 253,380 - +0.5 253,426 + -1.0 253,427 - +0.6 253,427 - -0.4 253,441 - -1.3 253,473 + +0.0 253,474 - -0.5 253,474 - +1.0 253,474 - -1.0 253,474 - -1.5 253,474 - +0.2 253,474 - -0.4 253,477 - -1.0 253,477 - -2.0 253,492 - +1.6 253,494 + +0.4 253,495 - +2.6 253,518 + +1.0
Or see this region's nucleotide sequence