Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4013 and Pf1N1B4_4014 are separated by 183 nucleotides Pf1N1B4_4014 and Pf1N1B4_4015 overlap by 11 nucleotides Pf1N1B4_4015 and Pf1N1B4_4016 are separated by 236 nucleotides
Pf1N1B4_4013 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 4,463,669 to 4,465,411
_4013
Pf1N1B4_4014 - FIG00954026: hypothetical protein, at 4,465,595 to 4,465,909
_4014
Pf1N1B4_4015 - FIG00958844: hypothetical protein, at 4,465,899 to 4,466,249
_4015
Pf1N1B4_4016 - Heavy metal sensor histidine kinase, at 4,466,486 to 4,467,889
_4016
Position (kb)
4465
4466
4467 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4464.929 kb on + strand, within Pf1N1B4_4013 at 4464.930 kb on - strand, within Pf1N1B4_4013 at 4464.930 kb on - strand at 4465.064 kb on + strand, within Pf1N1B4_4013 at 4465.251 kb on + strand at 4465.251 kb on + strand at 4465.252 kb on - strand at 4465.252 kb on - strand at 4465.351 kb on + strand at 4465.370 kb on + strand at 4465.370 kb on + strand at 4465.370 kb on + strand at 4465.409 kb on + strand at 4465.409 kb on + strand at 4465.410 kb on - strand at 4465.504 kb on + strand at 4465.505 kb on - strand at 4465.505 kb on - strand at 4465.521 kb on + strand at 4465.521 kb on + strand at 4465.522 kb on - strand at 4465.534 kb on + strand at 4465.535 kb on - strand at 4465.673 kb on - strand, within Pf1N1B4_4014 at 4465.673 kb on - strand, within Pf1N1B4_4014 at 4465.673 kb on - strand, within Pf1N1B4_4014 at 4465.673 kb on - strand, within Pf1N1B4_4014 at 4465.673 kb on - strand, within Pf1N1B4_4014 at 4465.728 kb on + strand, within Pf1N1B4_4014 at 4465.729 kb on - strand, within Pf1N1B4_4014 at 4465.729 kb on - strand, within Pf1N1B4_4014 at 4465.729 kb on - strand, within Pf1N1B4_4014 at 4465.729 kb on - strand, within Pf1N1B4_4014 at 4465.935 kb on - strand, within Pf1N1B4_4015 at 4465.935 kb on - strand at 4465.937 kb on - strand, within Pf1N1B4_4015 at 4465.938 kb on + strand, within Pf1N1B4_4015 at 4465.938 kb on + strand, within Pf1N1B4_4015 at 4465.938 kb on + strand, within Pf1N1B4_4015 at 4465.938 kb on + strand, within Pf1N1B4_4015 at 4465.938 kb on + strand at 4465.939 kb on - strand, within Pf1N1B4_4015 at 4465.939 kb on - strand, within Pf1N1B4_4015 at 4465.999 kb on + strand, within Pf1N1B4_4015 at 4466.001 kb on + strand, within Pf1N1B4_4015 at 4466.001 kb on + strand, within Pf1N1B4_4015 at 4466.002 kb on - strand, within Pf1N1B4_4015 at 4466.045 kb on - strand, within Pf1N1B4_4015 at 4466.120 kb on + strand, within Pf1N1B4_4015 at 4466.121 kb on - strand, within Pf1N1B4_4015 at 4466.197 kb on + strand, within Pf1N1B4_4015 at 4466.293 kb on + strand at 4466.294 kb on - strand at 4466.338 kb on + strand at 4466.339 kb on - strand at 4466.339 kb on - strand at 4466.363 kb on + strand at 4466.363 kb on + strand at 4466.364 kb on - strand at 4466.364 kb on - strand at 4466.487 kb on + strand at 4466.488 kb on - strand at 4466.979 kb on - strand at 4467.052 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 4,464,929 + Pf1N1B4_4013 0.72 -1.7 4,464,930 - Pf1N1B4_4013 0.72 -0.9 4,464,930 - +1.2 4,465,064 + Pf1N1B4_4013 0.80 +0.1 4,465,251 + -1.3 4,465,251 + -0.3 4,465,252 - -0.3 4,465,252 - +0.1 4,465,351 + -0.3 4,465,370 + +1.2 4,465,370 + -0.6 4,465,370 + +0.4 4,465,409 + +0.7 4,465,409 + -1.3 4,465,410 - +0.7 4,465,504 + +1.0 4,465,505 - +0.2 4,465,505 - +0.2 4,465,521 + +0.7 4,465,521 + +0.5 4,465,522 - +2.0 4,465,534 + -0.9 4,465,535 - +0.2 4,465,673 - Pf1N1B4_4014 0.25 -1.1 4,465,673 - Pf1N1B4_4014 0.25 +0.2 4,465,673 - Pf1N1B4_4014 0.25 +0.7 4,465,673 - Pf1N1B4_4014 0.25 -0.5 4,465,673 - Pf1N1B4_4014 0.25 -0.8 4,465,728 + Pf1N1B4_4014 0.42 -0.3 4,465,729 - Pf1N1B4_4014 0.43 +0.3 4,465,729 - Pf1N1B4_4014 0.43 -0.8 4,465,729 - Pf1N1B4_4014 0.43 +0.7 4,465,729 - Pf1N1B4_4014 0.43 -1.3 4,465,935 - Pf1N1B4_4015 0.10 -0.1 4,465,935 - -1.3 4,465,937 - Pf1N1B4_4015 0.11 -1.3 4,465,938 + Pf1N1B4_4015 0.11 -2.2 4,465,938 + Pf1N1B4_4015 0.11 -1.1 4,465,938 + Pf1N1B4_4015 0.11 -1.9 4,465,938 + Pf1N1B4_4015 0.11 -1.9 4,465,938 + +1.7 4,465,939 - Pf1N1B4_4015 0.11 -1.3 4,465,939 - Pf1N1B4_4015 0.11 -0.1 4,465,999 + Pf1N1B4_4015 0.28 -1.1 4,466,001 + Pf1N1B4_4015 0.29 -1.4 4,466,001 + Pf1N1B4_4015 0.29 -1.3 4,466,002 - Pf1N1B4_4015 0.29 -0.3 4,466,045 - Pf1N1B4_4015 0.42 -0.0 4,466,120 + Pf1N1B4_4015 0.63 -0.3 4,466,121 - Pf1N1B4_4015 0.63 -1.3 4,466,197 + Pf1N1B4_4015 0.85 +1.2 4,466,293 + -0.3 4,466,294 - +0.2 4,466,338 + +1.0 4,466,339 - -0.3 4,466,339 - -0.9 4,466,363 + -0.8 4,466,363 + -1.3 4,466,364 - -0.3 4,466,364 - -0.8 4,466,487 + +2.0 4,466,488 - -1.3 4,466,979 - -0.3 4,467,052 - -0.9
Or see this region's nucleotide sequence