Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3984 and Pf1N1B4_3985 overlap by 1 nucleotides Pf1N1B4_3985 and Pf1N1B4_3986 are separated by 115 nucleotides
Pf1N1B4_3984 - 3-methylcrotonyl-CoA carboxylase, alpha (biotin-containing) subunit (EC 6.4.1.4) (from data) , at 4,424,874 to 4,426,829
_3984
Pf1N1B4_3985 - Methylglutaconyl-CoA hydratase (EC 4.2.1.18), at 4,426,829 to 4,427,644
_3985
Pf1N1B4_3986 - Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4), at 4,427,760 to 4,429,367
_3986
Position (kb)
4426
4427
4428 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4426.015 kb on + strand, within Pf1N1B4_3984 at 4426.150 kb on + strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.151 kb on - strand, within Pf1N1B4_3984 at 4426.152 kb on + strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.153 kb on - strand, within Pf1N1B4_3984 at 4426.213 kb on + strand, within Pf1N1B4_3984 at 4426.214 kb on - strand, within Pf1N1B4_3984 at 4426.639 kb on + strand at 4426.640 kb on - strand at 4426.640 kb on - strand at 4427.321 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.361 kb on + strand, within Pf1N1B4_3985 at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.891 kb on - strand at 4427.894 kb on - strand at 4428.104 kb on - strand, within Pf1N1B4_3986 at 4428.215 kb on - strand, within Pf1N1B4_3986 at 4428.392 kb on + strand, within Pf1N1B4_3986
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 4,426,015 + Pf1N1B4_3984 0.58 +0.1 4,426,150 + Pf1N1B4_3984 0.65 +0.7 4,426,151 - Pf1N1B4_3984 0.65 -0.3 4,426,151 - Pf1N1B4_3984 0.65 +2.3 4,426,151 - Pf1N1B4_3984 0.65 +0.9 4,426,151 - Pf1N1B4_3984 0.65 -1.1 4,426,151 - Pf1N1B4_3984 0.65 +1.3 4,426,152 + Pf1N1B4_3984 0.65 +0.3 4,426,153 - Pf1N1B4_3984 0.65 +0.7 4,426,153 - Pf1N1B4_3984 0.65 -1.6 4,426,213 + Pf1N1B4_3984 0.68 -0.9 4,426,214 - Pf1N1B4_3984 0.69 -0.3 4,426,639 + -0.0 4,426,640 - -0.9 4,426,640 - -1.3 4,427,321 + Pf1N1B4_3985 0.60 -0.9 4,427,361 + Pf1N1B4_3985 0.65 -1.9 4,427,361 + Pf1N1B4_3985 0.65 -0.8 4,427,361 + Pf1N1B4_3985 0.65 -0.3 4,427,891 - -1.1 4,427,891 - +1.7 4,427,891 - +1.0 4,427,891 - -0.9 4,427,894 - -0.3 4,428,104 - Pf1N1B4_3986 0.21 -0.8 4,428,215 - Pf1N1B4_3986 0.28 +0.2 4,428,392 + Pf1N1B4_3986 0.39 +0.7
Or see this region's nucleotide sequence