Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_349 and Pf1N1B4_350 are separated by 12 nucleotides Pf1N1B4_350 and Pf1N1B4_351 overlap by 1 nucleotides Pf1N1B4_351 and Pf1N1B4_352 are separated by 1 nucleotides
Pf1N1B4_349 - Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57), at 414,116 to 415,228
_349
Pf1N1B4_350 - Chorismate mutase I (EC 5.4.99.5) / Prephenate dehydratase (EC 4.2.1.51), at 415,241 to 416,335
_350
Pf1N1B4_351 - Phosphoserine aminotransferase (EC 2.6.1.52), at 416,335 to 417,213
_351
Pf1N1B4_352 - Phosphoserine aminotransferase (EC 2.6.1.52), at 417,215 to 417,427
_352
Position (kb) 415
416
417 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.396 kb on + strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.397 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.535 kb on - strand, within Pf1N1B4_349 at 414.585 kb on + strand, within Pf1N1B4_349 at 414.586 kb on - strand, within Pf1N1B4_349 at 414.622 kb on - strand, within Pf1N1B4_349 at 414.630 kb on + strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.631 kb on - strand, within Pf1N1B4_349 at 414.852 kb on + strand, within Pf1N1B4_349 at 414.904 kb on - strand, within Pf1N1B4_349 at 415.174 kb on - strand at 415.174 kb on - strand at 415.284 kb on - strand at 415.284 kb on - strand at 415.284 kb on - strand at 415.311 kb on - strand at 415.365 kb on + strand, within Pf1N1B4_350 at 415.365 kb on + strand, within Pf1N1B4_350 at 415.365 kb on + strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.366 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.376 kb on - strand, within Pf1N1B4_350 at 415.755 kb on + strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 415.756 kb on - strand, within Pf1N1B4_350 at 416.043 kb on + strand, within Pf1N1B4_350 at 416.226 kb on - strand at 416.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 414,396 + Pf1N1B4_349 0.25 -1.8 414,396 + Pf1N1B4_349 0.25 -2.2 414,397 - Pf1N1B4_349 0.25 -1.3 414,397 - Pf1N1B4_349 0.25 -1.0 414,535 - Pf1N1B4_349 0.38 -1.6 414,535 - Pf1N1B4_349 0.38 -0.2 414,585 + Pf1N1B4_349 0.42 -2.8 414,586 - Pf1N1B4_349 0.42 +0.3 414,622 - Pf1N1B4_349 0.45 +0.4 414,630 + Pf1N1B4_349 0.46 -1.2 414,631 - Pf1N1B4_349 0.46 -1.2 414,631 - Pf1N1B4_349 0.46 -1.5 414,631 - Pf1N1B4_349 0.46 +1.1 414,631 - Pf1N1B4_349 0.46 +0.8 414,852 + Pf1N1B4_349 0.66 -0.2 414,904 - Pf1N1B4_349 0.71 +0.6 415,174 - -1.5 415,174 - -2.8 415,284 - -2.2 415,284 - -2.5 415,284 - -3.9 415,311 - -0.2 415,365 + Pf1N1B4_350 0.11 -3.9 415,365 + Pf1N1B4_350 0.11 -2.5 415,365 + Pf1N1B4_350 0.11 -0.9 415,366 - Pf1N1B4_350 0.11 -3.9 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 -0.6 415,366 - Pf1N1B4_350 0.11 -3.4 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 -1.2 415,366 - Pf1N1B4_350 0.11 -1.2 415,366 - Pf1N1B4_350 0.11 -2.5 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 -2.8 415,366 - Pf1N1B4_350 0.11 -1.5 415,366 - Pf1N1B4_350 0.11 -2.8 415,366 - Pf1N1B4_350 0.11 +0.4 415,366 - Pf1N1B4_350 0.11 -1.8 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 -1.2 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 -2.2 415,366 - Pf1N1B4_350 0.11 +0.4 415,376 - Pf1N1B4_350 0.12 -0.2 415,376 - Pf1N1B4_350 0.12 -1.8 415,376 - Pf1N1B4_350 0.12 -2.5 415,755 + Pf1N1B4_350 0.47 -3.8 415,756 - Pf1N1B4_350 0.47 -2.8 415,756 - -0.2 415,756 - Pf1N1B4_350 0.47 -1.2 415,756 - Pf1N1B4_350 0.47 -0.2 415,756 - Pf1N1B4_350 0.47 -0.8 415,756 - Pf1N1B4_350 0.47 -0.2 415,756 - Pf1N1B4_350 0.47 -3.4 416,043 + Pf1N1B4_350 0.73 -3.9 416,226 - -2.2 416,226 - -1.8
Or see this region's nucleotide sequence