Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1014 and Pf1N1B4_1015 are separated by 235 nucleotides Pf1N1B4_1015 and Pf1N1B4_1016 overlap by 4 nucleotides Pf1N1B4_1016 and Pf1N1B4_1017 are separated by 359 nucleotides Pf1N1B4_1017 and Pf1N1B4_1018 are separated by 66 nucleotides
Pf1N1B4_1014 - Serine transporter, at 1,150,147 to 1,151,448
_1014
Pf1N1B4_1015 - FIG00954638: hypothetical protein, at 1,151,684 to 1,151,998
_1015
Pf1N1B4_1016 - FIG00954263: hypothetical protein, at 1,151,995 to 1,152,495
_1016
Pf1N1B4_1017 - FIG00957554: hypothetical protein, at 1,152,855 to 1,153,220
_1017
Pf1N1B4_1018 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 1,153,287 to 1,154,396
_1018
Position (kb)
1151
1152
1153 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1151.029 kb on + strand, within Pf1N1B4_1014 at 1151.065 kb on + strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014 at 1151.066 kb on - strand, within Pf1N1B4_1014 at 1151.119 kb on + strand, within Pf1N1B4_1014 at 1151.119 kb on + strand, within Pf1N1B4_1014 at 1151.119 kb on + strand, within Pf1N1B4_1014 at 1151.119 kb on + strand, within Pf1N1B4_1014 at 1151.120 kb on - strand at 1151.120 kb on - strand, within Pf1N1B4_1014 at 1151.120 kb on - strand, within Pf1N1B4_1014 at 1151.120 kb on - strand, within Pf1N1B4_1014 at 1151.120 kb on - strand, within Pf1N1B4_1014 at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.332 kb on + strand at 1151.333 kb on - strand at 1151.333 kb on - strand at 1151.431 kb on + strand at 1151.431 kb on + strand at 1151.431 kb on + strand at 1151.431 kb on + strand at 1151.548 kb on + strand at 1151.549 kb on - strand at 1151.571 kb on + strand at 1151.572 kb on - strand at 1151.621 kb on + strand at 1151.621 kb on + strand at 1151.629 kb on + strand at 1151.629 kb on + strand at 1151.629 kb on + strand at 1151.629 kb on + strand at 1151.629 kb on + strand at 1151.707 kb on + strand at 1151.729 kb on + strand, within Pf1N1B4_1015 at 1151.996 kb on + strand at 1151.996 kb on + strand at 1151.996 kb on + strand at 1151.997 kb on - strand at 1152.132 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand, within Pf1N1B4_1016 at 1152.146 kb on + strand at 1152.147 kb on - strand, within Pf1N1B4_1016 at 1152.147 kb on - strand, within Pf1N1B4_1016 at 1152.147 kb on - strand, within Pf1N1B4_1016 at 1152.147 kb on - strand, within Pf1N1B4_1016 at 1152.147 kb on - strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.224 kb on + strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.225 kb on - strand, within Pf1N1B4_1016 at 1152.263 kb on + strand, within Pf1N1B4_1016 at 1152.263 kb on + strand, within Pf1N1B4_1016 at 1152.264 kb on - strand, within Pf1N1B4_1016 at 1152.283 kb on + strand, within Pf1N1B4_1016 at 1152.284 kb on - strand, within Pf1N1B4_1016 at 1152.293 kb on + strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.407 kb on + strand, within Pf1N1B4_1016 at 1152.408 kb on - strand, within Pf1N1B4_1016 at 1152.746 kb on + strand at 1152.746 kb on + strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.751 kb on + strand at 1152.751 kb on + strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.785 kb on + strand at 1152.800 kb on - strand at 1152.800 kb on - strand at 1152.804 kb on - strand at 1152.812 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.873 kb on + strand at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand at 1153.026 kb on + strand at 1153.027 kb on - strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.219 kb on - strand at 1153.219 kb on - strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.239 kb on - strand at 1153.288 kb on + strand at 1153.288 kb on + strand at 1153.289 kb on - strand at 1153.289 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,151,029 + Pf1N1B4_1014 0.68 -1.9 1,151,065 + Pf1N1B4_1014 0.71 -0.3 1,151,066 - Pf1N1B4_1014 0.71 -2.2 1,151,066 - Pf1N1B4_1014 0.71 +0.7 1,151,119 + Pf1N1B4_1014 0.75 +0.4 1,151,119 + Pf1N1B4_1014 0.75 +0.1 1,151,119 + Pf1N1B4_1014 0.75 +0.1 1,151,119 + Pf1N1B4_1014 0.75 +1.7 1,151,120 - +0.5 1,151,120 - Pf1N1B4_1014 0.75 -0.9 1,151,120 - Pf1N1B4_1014 0.75 -1.3 1,151,120 - Pf1N1B4_1014 0.75 +0.7 1,151,120 - Pf1N1B4_1014 0.75 -1.1 1,151,332 + +0.1 1,151,332 + +0.6 1,151,332 + -0.6 1,151,332 + -0.8 1,151,332 + -0.3 1,151,332 + +0.1 1,151,332 + +0.1 1,151,333 - -0.1 1,151,333 - +0.1 1,151,431 + +0.1 1,151,431 + -0.6 1,151,431 + -0.5 1,151,431 + -0.3 1,151,548 + +0.8 1,151,549 - -0.4 1,151,571 + +0.7 1,151,572 - -0.9 1,151,621 + -0.5 1,151,621 + +1.1 1,151,629 + -0.2 1,151,629 + -1.9 1,151,629 + -1.4 1,151,629 + -0.1 1,151,629 + +0.3 1,151,707 + -0.9 1,151,729 + Pf1N1B4_1015 0.14 +0.7 1,151,996 + +1.7 1,151,996 + +0.7 1,151,996 + -0.3 1,151,997 - -1.2 1,152,132 + Pf1N1B4_1016 0.27 -0.9 1,152,146 + Pf1N1B4_1016 0.30 -1.2 1,152,146 + Pf1N1B4_1016 0.30 +0.3 1,152,146 + Pf1N1B4_1016 0.30 -1.2 1,152,146 + Pf1N1B4_1016 0.30 -0.2 1,152,146 + Pf1N1B4_1016 0.30 -0.3 1,152,146 + Pf1N1B4_1016 0.30 -1.5 1,152,146 + Pf1N1B4_1016 0.30 -1.9 1,152,146 + Pf1N1B4_1016 0.30 -1.1 1,152,146 + Pf1N1B4_1016 0.30 +1.1 1,152,146 + Pf1N1B4_1016 0.30 -1.2 1,152,146 + +0.1 1,152,147 - Pf1N1B4_1016 0.30 -0.6 1,152,147 - Pf1N1B4_1016 0.30 -1.7 1,152,147 - Pf1N1B4_1016 0.30 -0.9 1,152,147 - Pf1N1B4_1016 0.30 +0.1 1,152,147 - Pf1N1B4_1016 0.30 -3.1 1,152,224 + Pf1N1B4_1016 0.46 -2.9 1,152,224 + Pf1N1B4_1016 0.46 -2.9 1,152,224 + Pf1N1B4_1016 0.46 -0.9 1,152,224 + +0.1 1,152,224 + Pf1N1B4_1016 0.46 -1.4 1,152,224 + Pf1N1B4_1016 0.46 -1.5 1,152,224 + Pf1N1B4_1016 0.46 -0.9 1,152,224 + Pf1N1B4_1016 0.46 -2.5 1,152,224 + Pf1N1B4_1016 0.46 -0.8 1,152,224 + Pf1N1B4_1016 0.46 -0.9 1,152,225 - Pf1N1B4_1016 0.46 +1.3 1,152,225 - Pf1N1B4_1016 0.46 -0.9 1,152,225 - Pf1N1B4_1016 0.46 -0.2 1,152,225 - Pf1N1B4_1016 0.46 -0.6 1,152,225 - Pf1N1B4_1016 0.46 -0.7 1,152,225 - Pf1N1B4_1016 0.46 +0.1 1,152,225 - Pf1N1B4_1016 0.46 -1.3 1,152,225 - Pf1N1B4_1016 0.46 -1.9 1,152,225 - Pf1N1B4_1016 0.46 -1.5 1,152,263 + Pf1N1B4_1016 0.53 -2.6 1,152,263 + Pf1N1B4_1016 0.53 +0.7 1,152,264 - Pf1N1B4_1016 0.54 -0.7 1,152,283 + Pf1N1B4_1016 0.57 +0.7 1,152,284 - Pf1N1B4_1016 0.58 +0.1 1,152,293 + Pf1N1B4_1016 0.59 -1.9 1,152,294 - Pf1N1B4_1016 0.60 -2.5 1,152,294 - Pf1N1B4_1016 0.60 -1.1 1,152,294 - Pf1N1B4_1016 0.60 -2.2 1,152,294 - Pf1N1B4_1016 0.60 -1.9 1,152,294 - Pf1N1B4_1016 0.60 +0.1 1,152,294 - Pf1N1B4_1016 0.60 -0.7 1,152,407 + Pf1N1B4_1016 0.82 +0.1 1,152,408 - Pf1N1B4_1016 0.82 +0.1 1,152,746 + +1.1 1,152,746 + +1.1 1,152,747 - +1.1 1,152,747 - -0.5 1,152,747 - -0.0 1,152,747 - +1.7 1,152,751 + -0.5 1,152,751 + +1.7 1,152,752 - -1.6 1,152,752 - -1.9 1,152,752 - +3.4 1,152,752 - +1.1 1,152,752 - -0.3 1,152,785 + +0.1 1,152,800 - -0.5 1,152,800 - -0.3 1,152,804 - -0.5 1,152,812 + +0.1 1,152,829 + -2.7 1,152,829 + -0.9 1,152,829 + -0.9 1,152,829 + +1.1 1,152,829 + +0.1 1,152,829 + +2.1 1,152,829 + -0.4 1,152,830 - -2.7 1,152,830 - +1.1 1,152,830 - -2.2 1,152,830 - +0.5 1,152,830 - -0.9 1,152,871 + +0.1 1,152,871 + -0.1 1,152,871 + +0.1 1,152,872 - +0.1 1,152,872 - -0.3 1,152,872 - -0.4 1,152,872 - -1.2 1,152,872 - +1.2 1,152,873 + -0.2 1,153,026 + Pf1N1B4_1017 0.47 +0.1 1,153,026 + Pf1N1B4_1017 0.47 +1.4 1,153,026 + Pf1N1B4_1017 0.47 +0.1 1,153,026 + Pf1N1B4_1017 0.47 -0.4 1,153,026 + Pf1N1B4_1017 0.47 -1.9 1,153,026 + Pf1N1B4_1017 0.47 +0.7 1,153,026 + -0.9 1,153,026 + +0.1 1,153,027 - Pf1N1B4_1017 0.47 -0.5 1,153,048 + Pf1N1B4_1017 0.53 +0.7 1,153,048 + Pf1N1B4_1017 0.53 +0.1 1,153,048 + Pf1N1B4_1017 0.53 +0.1 1,153,048 + +0.1 1,153,048 + Pf1N1B4_1017 0.53 +0.5 1,153,049 - Pf1N1B4_1017 0.53 +0.5 1,153,049 - Pf1N1B4_1017 0.53 +0.5 1,153,049 - Pf1N1B4_1017 0.53 +1.4 1,153,150 + Pf1N1B4_1017 0.81 +1.8 1,153,150 + Pf1N1B4_1017 0.81 -0.9 1,153,150 + Pf1N1B4_1017 0.81 +0.3 1,153,150 + -0.9 1,153,218 + -0.6 1,153,218 + -0.1 1,153,218 + -2.7 1,153,218 + +0.1 1,153,218 + -0.2 1,153,219 - +0.1 1,153,219 - -0.0 1,153,238 + -0.6 1,153,238 + +1.1 1,153,238 + +0.4 1,153,239 - +0.1 1,153,288 + +0.7 1,153,288 + -1.5 1,153,289 - -0.6 1,153,289 - +1.1
Or see this region's nucleotide sequence