Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08420

Experiment: acetate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08415 and BWI76_RS08420 are separated by 14 nucleotidesBWI76_RS08420 and BWI76_RS08425 are separated by 99 nucleotides BWI76_RS08415: BWI76_RS08415 - 2-oxoglutarate dehydrogenase subunit E1, at 1,779,225 to 1,782,032 _RS08415 BWI76_RS08420: BWI76_RS08420 - dihydrolipoamide succinyltransferase, at 1,782,047 to 1,783,270 _RS08420 BWI76_RS08425: BWI76_RS08425 - succinyl-CoA ligase subunit beta, at 1,783,370 to 1,784,536 _RS08425 Position (kb) 1782 1783 1784Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 1783.362 kb on - strandat 1783.417 kb on - strandat 1783.706 kb on + strand, within BWI76_RS08425at 1784.119 kb on + strand, within BWI76_RS08425

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Per-strain Table

Position Strand Gene LocusTag Fraction acetate (C)
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1,783,362 - -2.0
1,783,417 - -2.5
1,783,706 + BWI76_RS08425 0.29 -5.8
1,784,119 + BWI76_RS08425 0.64 -5.2

Or see this region's nucleotide sequence