Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxK and Shewana3_2584 are separated by 0 nucleotides Shewana3_2584 and Shewana3_2585 are separated by 32 nucleotides
Shewana3_2582: Shewana3_2582 - hypothetical protein (RefSeq), at 3,086,265 to 3,086,444
_2582
Shewana3_2583: lpxK - tetraacyldisaccharide 4'-kinase (RefSeq), at 3,086,434 to 3,087,441
lpxK
Shewana3_2584: Shewana3_2584 - lipid A ABC exporter, fused ATPase and inner membrane subunits MsbA (RefSeq), at 3,087,442 to 3,089,244
_2584
Shewana3_2585: Shewana3_2585 - DNA internalization-related competence protein ComEC/Rec2 (RefSeq), at 3,089,277 to 3,091,598
_2585
Position (kb)
3087
3088
3089
3090 Strain fitness (log2 ratio)
-1
0
1 at 3089.463 kb on - strand at 3089.467 kb on + strand at 3089.475 kb on - strand at 3089.901 kb on + strand, within Shewana3_2585 at 3089.995 kb on + strand, within Shewana3_2585 at 3090.003 kb on - strand, within Shewana3_2585 at 3090.120 kb on + strand, within Shewana3_2585 at 3090.128 kb on - strand, within Shewana3_2585 at 3090.191 kb on - strand, within Shewana3_2585 at 3090.225 kb on - strand, within Shewana3_2585
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,089,463 - +0.1 3,089,467 + +1.1 3,089,475 - +1.1 3,089,901 + Shewana3_2585 0.27 +0.1 3,089,995 + Shewana3_2585 0.31 +1.1 3,090,003 - Shewana3_2585 0.31 -1.2 3,090,120 + Shewana3_2585 0.36 -0.5 3,090,128 - Shewana3_2585 0.37 +0.1 3,090,191 - Shewana3_2585 0.39 +0.1 3,090,225 - Shewana3_2585 0.41 -1.6
Or see this region's nucleotide sequence