Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2532 and Shewana3_2533 are separated by 0 nucleotides Shewana3_2533 and Shewana3_2534 overlap by 1 nucleotides Shewana3_2534 and Shewana3_2535 overlap by 11 nucleotides Shewana3_2535 and Shewana3_2536 are separated by 170 nucleotides
Shewana3_2532: Shewana3_2532 - TolA family protein (RefSeq), at 3,028,872 to 3,029,894
_2532
Shewana3_2533: Shewana3_2533 - biopolymer transport protein ExbD/TolR (RefSeq), at 3,029,895 to 3,030,329
_2533
Shewana3_2534: Shewana3_2534 - MotA/TolQ/ExbB proton channel (RefSeq), at 3,030,329 to 3,031,015
_2534
Shewana3_2535: Shewana3_2535 - 4-hydroxybenzoyl-CoA thioesterase (RefSeq), at 3,031,005 to 3,031,406
_2535
Shewana3_2536: Shewana3_2536 - prolyl oligopeptidase (RefSeq), at 3,031,577 to 3,033,757
_2536
Position (kb)
3030
3031
3032 Strain fitness (log2 ratio)
-2
-1
0
1 at 3031.033 kb on - strand at 3031.056 kb on - strand, within Shewana3_2535 at 3031.056 kb on - strand, within Shewana3_2535 at 3031.078 kb on - strand, within Shewana3_2535 at 3031.291 kb on - strand, within Shewana3_2535 at 3031.306 kb on + strand, within Shewana3_2535 at 3031.360 kb on - strand, within Shewana3_2535 at 3031.378 kb on - strand at 3031.644 kb on - strand at 3031.689 kb on + strand at 3031.895 kb on + strand, within Shewana3_2536 at 3031.995 kb on - strand, within Shewana3_2536
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 3,031,033 - +1.0 3,031,056 - Shewana3_2535 0.13 +1.0 3,031,056 - Shewana3_2535 0.13 -0.1 3,031,078 - Shewana3_2535 0.18 +0.0 3,031,291 - Shewana3_2535 0.71 +0.0 3,031,306 + Shewana3_2535 0.75 +1.0 3,031,360 - Shewana3_2535 0.88 -2.3 3,031,378 - -2.0 3,031,644 - -0.2 3,031,689 + +1.0 3,031,895 + Shewana3_2536 0.15 +1.0 3,031,995 - Shewana3_2536 0.19 -1.8
Or see this region's nucleotide sequence