Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ushA and Shewana3_2455 are separated by 364 nucleotides Shewana3_2455 and Shewana3_2456 are separated by 52 nucleotides Shewana3_2456 and Shewana3_2457 are separated by 344 nucleotides
Shewana3_2454: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,941,773 to 2,943,494
ushA
Shewana3_2455: Shewana3_2455 - phospholipase/carboxylesterase (RefSeq), at 2,943,859 to 2,944,524
_2455
Shewana3_2456: Shewana3_2456 - hypothetical protein (RefSeq), at 2,944,577 to 2,944,846
_2456
Shewana3_2457: Shewana3_2457 - peptidase M1, membrane alanine aminopeptidase (RefSeq), at 2,945,191 to 2,947,119
_2457
Position (kb)
2943
2944
2945 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2943.092 kb on - strand, within ushA at 2943.180 kb on + strand, within ushA at 2943.389 kb on - strand at 2943.437 kb on - strand at 2943.475 kb on + strand at 2944.018 kb on - strand, within Shewana3_2455 at 2944.192 kb on + strand, within Shewana3_2455 at 2944.254 kb on + strand, within Shewana3_2455 at 2944.262 kb on - strand, within Shewana3_2455 at 2944.262 kb on - strand, within Shewana3_2455 at 2944.265 kb on + strand, within Shewana3_2455 at 2944.281 kb on - strand, within Shewana3_2455 at 2944.398 kb on - strand, within Shewana3_2455 at 2944.698 kb on - strand, within Shewana3_2456 at 2944.711 kb on - strand, within Shewana3_2456 at 2944.776 kb on + strand, within Shewana3_2456 at 2944.910 kb on + strand at 2944.910 kb on + strand at 2944.918 kb on - strand at 2944.918 kb on - strand at 2944.918 kb on - strand at 2944.995 kb on - strand at 2945.212 kb on - strand at 2945.235 kb on - strand at 2945.273 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,943,092 - ushA Shewana3_2454 0.77 +1.8 2,943,180 + ushA Shewana3_2454 0.82 +1.0 2,943,389 - +1.4 2,943,437 - +0.3 2,943,475 + +1.0 2,944,018 - Shewana3_2455 0.24 -2.0 2,944,192 + Shewana3_2455 0.50 +1.0 2,944,254 + Shewana3_2455 0.59 +1.0 2,944,262 - Shewana3_2455 0.61 -3.3 2,944,262 - Shewana3_2455 0.61 -3.2 2,944,265 + Shewana3_2455 0.61 +1.0 2,944,281 - Shewana3_2455 0.63 +0.1 2,944,398 - Shewana3_2455 0.81 -2.8 2,944,698 - Shewana3_2456 0.45 +0.8 2,944,711 - Shewana3_2456 0.50 +0.3 2,944,776 + Shewana3_2456 0.74 +1.0 2,944,910 + +1.0 2,944,910 + +1.0 2,944,918 - -1.0 2,944,918 - -1.0 2,944,918 - +0.8 2,944,995 - +1.1 2,945,212 - +0.5 2,945,235 - +1.0 2,945,273 - -1.6
Or see this region's nucleotide sequence