Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1920 and Shewana3_1921 are separated by 1 nucleotides Shewana3_1921 and Shewana3_1922 are separated by 215 nucleotides Shewana3_1922 and Shewana3_1923 are separated by 79 nucleotides
Shewana3_1920: Shewana3_1920 - hypothetical protein (RefSeq), at 2,282,229 to 2,283,893
_1920
Shewana3_1921: Shewana3_1921 - hypothetical protein (RefSeq), at 2,283,895 to 2,284,494
_1921
Shewana3_1922: Shewana3_1922 - sodium/sulphate symporter (RefSeq), at 2,284,710 to 2,286,098
_1922
Shewana3_1923: Shewana3_1923 - AraC family transcriptional regulator (RefSeq), at 2,286,178 to 2,286,990
_1923
Position (kb)
2284
2285
2286
2287 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2283.777 kb on - strand at 2283.963 kb on - strand, within Shewana3_1921 at 2284.463 kb on + strand at 2284.463 kb on + strand at 2284.486 kb on + strand at 2284.588 kb on + strand at 2284.599 kb on + strand at 2284.707 kb on + strand at 2284.735 kb on + strand at 2284.776 kb on - strand at 2284.792 kb on - strand at 2284.922 kb on + strand, within Shewana3_1922 at 2285.094 kb on + strand, within Shewana3_1922 at 2285.237 kb on + strand, within Shewana3_1922 at 2285.460 kb on - strand, within Shewana3_1922 at 2285.511 kb on + strand, within Shewana3_1922 at 2285.984 kb on - strand at 2286.405 kb on + strand, within Shewana3_1923 at 2286.490 kb on + strand, within Shewana3_1923 at 2286.490 kb on + strand, within Shewana3_1923 at 2286.498 kb on - strand, within Shewana3_1923 at 2286.502 kb on - strand, within Shewana3_1923 at 2286.534 kb on - strand, within Shewana3_1923 at 2286.617 kb on + strand, within Shewana3_1923 at 2286.630 kb on + strand, within Shewana3_1923 at 2286.847 kb on - strand, within Shewana3_1923 at 2286.877 kb on - strand, within Shewana3_1923 at 2286.922 kb on + strand at 2287.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 2,283,777 - +0.9 2,283,963 - Shewana3_1921 0.11 +0.3 2,284,463 + -2.8 2,284,463 + -1.6 2,284,486 + -0.7 2,284,588 + -2.6 2,284,599 + +0.9 2,284,707 + +0.9 2,284,735 + +1.9 2,284,776 - -3.1 2,284,792 - -2.6 2,284,922 + Shewana3_1922 0.15 -0.4 2,285,094 + Shewana3_1922 0.28 +0.9 2,285,237 + Shewana3_1922 0.38 +0.9 2,285,460 - Shewana3_1922 0.54 +0.9 2,285,511 + Shewana3_1922 0.58 +0.9 2,285,984 - -2.4 2,286,405 + Shewana3_1923 0.28 -0.1 2,286,490 + Shewana3_1923 0.38 -1.1 2,286,490 + Shewana3_1923 0.38 +0.9 2,286,498 - Shewana3_1923 0.39 +0.9 2,286,502 - Shewana3_1923 0.40 +0.9 2,286,534 - Shewana3_1923 0.44 +0.9 2,286,617 + Shewana3_1923 0.54 -0.1 2,286,630 + Shewana3_1923 0.56 -0.7 2,286,847 - Shewana3_1923 0.82 +0.9 2,286,877 - Shewana3_1923 0.86 +0.9 2,286,922 + -1.4 2,287,086 - -0.1
Or see this region's nucleotide sequence