Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1712

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1711 and sucC are separated by 118 nucleotidessucC and Shewana3_1713 overlap by 1 nucleotides Shewana3_1711: Shewana3_1711 - 2-oxoglutarate dehydrogenase E2 component (RefSeq), at 2,015,520 to 2,016,713 _1711 Shewana3_1712: sucC - succinyl-CoA synthetase subunit beta (RefSeq), at 2,016,832 to 2,017,998 sucC Shewana3_1713: Shewana3_1713 - succinyl-CoA synthetase (ADP-forming) alpha subunit (RefSeq), at 2,017,998 to 2,018,870 _1713 Position (kb) 2016 2017 2018Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2017.453 kb on + strand, within sucCat 2017.864 kb on + strand, within sucCat 2018.052 kb on + strandat 2018.133 kb on + strand, within Shewana3_1713at 2018.154 kb on + strand, within Shewana3_1713at 2018.964 kb on + strandat 2018.972 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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2,017,453 + sucC Shewana3_1712 0.53 -2.1
2,017,864 + sucC Shewana3_1712 0.88 -0.2
2,018,052 + -2.4
2,018,133 + Shewana3_1713 0.15 -3.8
2,018,154 + Shewana3_1713 0.18 -1.1
2,018,964 + +0.4
2,018,972 - -0.2

Or see this region's nucleotide sequence