Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1171 and Shewana3_1172 are separated by 12 nucleotides Shewana3_1172 and Shewana3_1173 are separated by 53 nucleotides Shewana3_1173 and Shewana3_1174 overlap by 13 nucleotides
Shewana3_1171: Shewana3_1171 - cyclopropane-fatty-acyl-phospholipid synthase (RefSeq), at 1,381,737 to 1,382,993
_1171
Shewana3_1172: Shewana3_1172 - hypothetical protein (RefSeq), at 1,383,006 to 1,383,551
_1172
Shewana3_1173: Shewana3_1173 - hypothetical protein (RefSeq), at 1,383,605 to 1,384,177
_1173
Shewana3_1174: Shewana3_1174 - hypothetical protein (RefSeq), at 1,384,165 to 1,384,713
_1174
Position (kb)
1383
1384 Strain fitness (log2 ratio)
-1
0
1
2 at 1382.130 kb on - strand, within Shewana3_1171 at 1382.212 kb on + strand, within Shewana3_1171 at 1382.213 kb on - strand, within Shewana3_1171 at 1382.304 kb on - strand, within Shewana3_1171 at 1382.307 kb on + strand, within Shewana3_1171 at 1382.361 kb on + strand, within Shewana3_1171 at 1382.446 kb on + strand, within Shewana3_1171 at 1382.499 kb on + strand, within Shewana3_1171 at 1382.549 kb on - strand, within Shewana3_1171 at 1382.844 kb on + strand, within Shewana3_1171 at 1383.022 kb on + strand at 1383.087 kb on + strand, within Shewana3_1172 at 1383.187 kb on + strand, within Shewana3_1172 at 1383.396 kb on + strand, within Shewana3_1172 at 1383.532 kb on + strand at 1383.553 kb on + strand at 1383.667 kb on + strand, within Shewana3_1173 at 1383.732 kb on + strand, within Shewana3_1173 at 1383.777 kb on + strand, within Shewana3_1173 at 1383.785 kb on - strand, within Shewana3_1173 at 1384.207 kb on + strand at 1384.209 kb on - strand at 1384.475 kb on + strand, within Shewana3_1174 at 1384.538 kb on + strand, within Shewana3_1174
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 1,382,130 - Shewana3_1171 0.31 -0.7 1,382,212 + Shewana3_1171 0.38 +0.3 1,382,213 - Shewana3_1171 0.38 +1.3 1,382,304 - Shewana3_1171 0.45 +1.3 1,382,307 + Shewana3_1171 0.45 +1.6 1,382,361 + Shewana3_1171 0.50 +0.4 1,382,446 + Shewana3_1171 0.56 +0.3 1,382,499 + Shewana3_1171 0.61 -0.4 1,382,549 - Shewana3_1171 0.65 +1.3 1,382,844 + Shewana3_1171 0.88 -0.9 1,383,022 + +2.6 1,383,087 + Shewana3_1172 0.15 +2.3 1,383,187 + Shewana3_1172 0.33 -0.7 1,383,396 + Shewana3_1172 0.71 +0.4 1,383,532 + +0.2 1,383,553 + -1.4 1,383,667 + Shewana3_1173 0.11 +0.0 1,383,732 + Shewana3_1173 0.22 -1.6 1,383,777 + Shewana3_1173 0.30 +1.1 1,383,785 - Shewana3_1173 0.31 -0.7 1,384,207 + +0.3 1,384,209 - +1.5 1,384,475 + Shewana3_1174 0.56 -1.5 1,384,538 + Shewana3_1174 0.68 -1.7
Or see this region's nucleotide sequence