Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1121

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShewana3_1120 and surE overlap by 4 nucleotidessurE and Shewana3_1122 overlap by 4 nucleotidesShewana3_1122 and Shewana3_1123 are separated by 84 nucleotides Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894 _1120 Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640 surE Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272 _1122 Shewana3_1123: Shewana3_1123 - peptidase M23B (RefSeq), at 1,321,357 to 1,322,253 _1123 Position (kb) 1319 1320 1321Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1318.941 kb on - strand, within Shewana3_1120at 1319.026 kb on - strand, within Shewana3_1120at 1319.066 kb on + strand, within Shewana3_1120at 1319.074 kb on - strand, within Shewana3_1120at 1319.146 kb on + strand, within Shewana3_1120at 1319.262 kb on + strand, within Shewana3_1120at 1319.331 kb on + strand, within Shewana3_1120at 1319.333 kb on + strand, within Shewana3_1120at 1319.350 kb on + strand, within Shewana3_1120at 1319.584 kb on + strand, within Shewana3_1120at 1319.876 kb on + strandat 1319.886 kb on + strandat 1319.978 kb on + strand, within surEat 1320.132 kb on - strand, within surEat 1320.232 kb on + strand, within surEat 1320.397 kb on + strand, within surEat 1320.433 kb on + strand, within surEat 1320.441 kb on - strand, within surEat 1320.578 kb on + strandat 1320.728 kb on + strand, within Shewana3_1122at 1320.950 kb on + strand, within Shewana3_1122at 1321.345 kb on - strandat 1321.439 kb on - strandat 1321.544 kb on - strand, within Shewana3_1123

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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1,318,941 - Shewana3_1120 0.15 -0.7
1,319,026 - Shewana3_1120 0.23 +1.3
1,319,066 + Shewana3_1120 0.27 -0.5
1,319,074 - Shewana3_1120 0.27 -2.4
1,319,146 + Shewana3_1120 0.34 +1.3
1,319,262 + Shewana3_1120 0.44 +0.2
1,319,331 + Shewana3_1120 0.50 +0.7
1,319,333 + Shewana3_1120 0.50 -0.2
1,319,350 + Shewana3_1120 0.52 -1.6
1,319,584 + Shewana3_1120 0.72 -0.5
1,319,876 + +1.1
1,319,886 + -0.0
1,319,978 + surE Shewana3_1121 0.12 +1.6
1,320,132 - surE Shewana3_1121 0.32 +2.1
1,320,232 + surE Shewana3_1121 0.45 +2.4
1,320,397 + surE Shewana3_1121 0.67 +1.0
1,320,433 + surE Shewana3_1121 0.72 +0.8
1,320,441 - surE Shewana3_1121 0.73 -1.5
1,320,578 + +1.4
1,320,728 + Shewana3_1122 0.14 -2.8
1,320,950 + Shewana3_1122 0.49 +0.7
1,321,345 - -0.0
1,321,439 - +0.3
1,321,544 - Shewana3_1123 0.21 -0.3

Or see this region's nucleotide sequence