Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemE and Shewana3_0436 are separated by 377 nucleotides Shewana3_0436 and Shewana3_0437 are separated by 75 nucleotides Shewana3_0437 and Shewana3_0438 are separated by 3 nucleotides
Shewana3_0435: hemE - uroporphyrinogen decarboxylase (RefSeq), at 505,704 to 506,768
hemE
Shewana3_0436: Shewana3_0436 - diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s) (RefSeq), at 507,146 to 509,716
_0436
Shewana3_0437: Shewana3_0437 - short chain dehydrogenase (RefSeq), at 509,792 to 510,595
_0437
Shewana3_0438: Shewana3_0438 - hypothetical protein (RefSeq), at 510,599 to 510,823
_0438
Position (kb) 507
508
509
510 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 506.857 kb on - strand at 507.099 kb on - strand at 507.101 kb on + strand at 507.101 kb on + strand at 507.109 kb on - strand at 507.141 kb on - strand at 507.243 kb on + strand at 507.301 kb on + strand at 507.495 kb on + strand, within Shewana3_0436 at 507.631 kb on - strand, within Shewana3_0436 at 507.644 kb on + strand, within Shewana3_0436 at 507.846 kb on + strand, within Shewana3_0436 at 507.893 kb on - strand, within Shewana3_0436 at 507.996 kb on - strand, within Shewana3_0436 at 508.107 kb on - strand, within Shewana3_0436 at 508.150 kb on + strand, within Shewana3_0436 at 508.189 kb on + strand, within Shewana3_0436 at 508.192 kb on - strand, within Shewana3_0436 at 508.192 kb on - strand, within Shewana3_0436 at 508.259 kb on - strand, within Shewana3_0436 at 508.310 kb on - strand, within Shewana3_0436 at 508.615 kb on + strand, within Shewana3_0436 at 508.768 kb on + strand, within Shewana3_0436 at 508.819 kb on - strand, within Shewana3_0436 at 508.842 kb on - strand, within Shewana3_0436 at 508.842 kb on - strand, within Shewana3_0436 at 508.847 kb on + strand, within Shewana3_0436 at 508.912 kb on - strand, within Shewana3_0436 at 509.101 kb on - strand, within Shewana3_0436 at 509.118 kb on + strand, within Shewana3_0436 at 509.118 kb on + strand, within Shewana3_0436 at 509.520 kb on - strand at 509.524 kb on + strand at 509.633 kb on + strand at 509.638 kb on + strand at 509.849 kb on - strand at 509.991 kb on - strand, within Shewana3_0437 at 510.005 kb on + strand, within Shewana3_0437 at 510.039 kb on + strand, within Shewana3_0437 at 510.167 kb on + strand, within Shewana3_0437 at 510.175 kb on - strand, within Shewana3_0437 at 510.185 kb on - strand, within Shewana3_0437 at 510.202 kb on - strand, within Shewana3_0437 at 510.234 kb on - strand, within Shewana3_0437 at 510.399 kb on - strand, within Shewana3_0437 at 510.637 kb on - strand, within Shewana3_0438
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 506,857 - +0.2 507,099 - -3.5 507,101 + -1.0 507,101 + -0.0 507,109 - -1.6 507,141 - -2.9 507,243 + -2.0 507,301 + -3.8 507,495 + Shewana3_0436 0.14 -2.3 507,631 - Shewana3_0436 0.19 -3.6 507,644 + Shewana3_0436 0.19 -2.1 507,846 + Shewana3_0436 0.27 -5.4 507,893 - Shewana3_0436 0.29 -0.4 507,996 - Shewana3_0436 0.33 -0.0 508,107 - Shewana3_0436 0.37 -0.4 508,150 + Shewana3_0436 0.39 -2.9 508,189 + Shewana3_0436 0.41 -0.8 508,192 - Shewana3_0436 0.41 -0.4 508,192 - Shewana3_0436 0.41 -2.1 508,259 - Shewana3_0436 0.43 +1.6 508,310 - Shewana3_0436 0.45 -1.4 508,615 + Shewana3_0436 0.57 -3.9 508,768 + Shewana3_0436 0.63 -5.7 508,819 - Shewana3_0436 0.65 -2.0 508,842 - Shewana3_0436 0.66 +0.6 508,842 - Shewana3_0436 0.66 -3.1 508,847 + Shewana3_0436 0.66 -2.1 508,912 - Shewana3_0436 0.69 -2.2 509,101 - Shewana3_0436 0.76 +0.6 509,118 + Shewana3_0436 0.77 -2.2 509,118 + Shewana3_0436 0.77 -2.7 509,520 - -0.0 509,524 + -4.1 509,633 + -1.3 509,638 + -4.3 509,849 - +1.6 509,991 - Shewana3_0437 0.25 -1.6 510,005 + Shewana3_0437 0.26 -0.0 510,039 + Shewana3_0437 0.31 -1.1 510,167 + Shewana3_0437 0.47 -2.4 510,175 - Shewana3_0437 0.48 -1.6 510,185 - Shewana3_0437 0.49 -1.0 510,202 - Shewana3_0437 0.51 +0.6 510,234 - Shewana3_0437 0.55 -0.9 510,399 - Shewana3_0437 0.75 -2.2 510,637 - Shewana3_0438 0.17 -0.6
Or see this region's nucleotide sequence