Experiment: outer cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0383 and Shewana3_0384 overlap by 4 nucleotides Shewana3_0384 and Shewana3_0385 are separated by 60 nucleotides Shewana3_0385 and hemC are separated by 46 nucleotides
Shewana3_0383: Shewana3_0383 - HemY domain-containing protein (RefSeq), at 443,292 to 444,458
_0383
Shewana3_0384: Shewana3_0384 - protein of unknown function DUF513, hemX (RefSeq), at 444,455 to 445,696
_0384
Shewana3_0385: Shewana3_0385 - uroporphyrinogen III synthase HEM4 (RefSeq), at 445,757 to 446,455
_0385
Shewana3_0386: hemC - porphobilinogen deaminase (RefSeq), at 446,502 to 447,434
hemC
Position (kb) 444
445
446 Strain fitness (log2 ratio) -2
-1
0
1 at 443.490 kb on - strand, within Shewana3_0383 at 443.681 kb on + strand, within Shewana3_0383 at 443.717 kb on + strand, within Shewana3_0383 at 443.725 kb on - strand, within Shewana3_0383 at 443.749 kb on + strand, within Shewana3_0383 at 443.757 kb on - strand, within Shewana3_0383 at 443.840 kb on + strand, within Shewana3_0383 at 443.879 kb on - strand, within Shewana3_0383 at 444.130 kb on + strand, within Shewana3_0383 at 444.289 kb on + strand, within Shewana3_0383 at 444.333 kb on + strand, within Shewana3_0383 at 444.604 kb on - strand, within Shewana3_0384 at 444.605 kb on + strand, within Shewana3_0384 at 444.613 kb on - strand, within Shewana3_0384 at 444.755 kb on + strand, within Shewana3_0384 at 444.768 kb on - strand, within Shewana3_0384 at 444.769 kb on - strand, within Shewana3_0384 at 444.785 kb on - strand, within Shewana3_0384 at 444.913 kb on - strand, within Shewana3_0384 at 444.957 kb on - strand, within Shewana3_0384 at 445.084 kb on + strand, within Shewana3_0384 at 445.179 kb on + strand, within Shewana3_0384 at 445.200 kb on - strand, within Shewana3_0384 at 445.214 kb on + strand, within Shewana3_0384 at 445.315 kb on - strand, within Shewana3_0384 at 445.439 kb on - strand, within Shewana3_0384 at 445.588 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay remove 443,490 - Shewana3_0383 0.17 -0.2 443,681 + Shewana3_0383 0.33 -0.0 443,717 + Shewana3_0383 0.36 -1.4 443,725 - Shewana3_0383 0.37 -1.3 443,749 + Shewana3_0383 0.39 -0.7 443,757 - Shewana3_0383 0.40 -1.4 443,840 + Shewana3_0383 0.47 +1.6 443,879 - Shewana3_0383 0.50 -0.7 444,130 + Shewana3_0383 0.72 -0.9 444,289 + Shewana3_0383 0.85 -2.1 444,333 + Shewana3_0383 0.89 +0.6 444,604 - Shewana3_0384 0.12 -1.4 444,605 + Shewana3_0384 0.12 +0.6 444,613 - Shewana3_0384 0.13 -1.1 444,755 + Shewana3_0384 0.24 -1.4 444,768 - Shewana3_0384 0.25 -0.7 444,769 - Shewana3_0384 0.25 -0.4 444,785 - Shewana3_0384 0.27 +0.6 444,913 - Shewana3_0384 0.37 +0.4 444,957 - Shewana3_0384 0.40 -2.2 445,084 + Shewana3_0384 0.51 -2.1 445,179 + Shewana3_0384 0.58 +0.6 445,200 - Shewana3_0384 0.60 -0.9 445,214 + Shewana3_0384 0.61 -0.4 445,315 - Shewana3_0384 0.69 +1.6 445,439 - Shewana3_0384 0.79 +1.6 445,588 + -2.0
Or see this region's nucleotide sequence