Strain Fitness in Shewanella sp. ANA-3 around Shewana3_0001

Experiment: outer cut, LB soft agar motility assay

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgidB and Shewana3_0002 are separated by 117 nucleotides Shewana3_0001: gidB - 16S rRNA methyltransferase GidB (RefSeq), at 39 to 659 gidB Shewana3_0002: Shewana3_0002 - tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA (RefSeq), at 777 to 2,666 _0002 Position (kb) 0 1Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 0.148 kb on + strand, within gidBat 0.279 kb on - strand, within gidBat 0.331 kb on - strand, within gidBat 0.377 kb on + strand, within gidBat 0.385 kb on - strand, within gidBat 0.385 kb on - strand, within gidBat 0.439 kb on - strand, within gidBat 0.479 kb on + strand, within gidBat 0.479 kb on + strand, within gidBat 0.479 kb on + strand, within gidBat 0.479 kb on + strand, within gidBat 0.479 kb on + strand, within gidBat 0.487 kb on - strand, within gidBat 0.487 kb on - strand, within gidBat 0.516 kb on - strand, within gidBat 0.547 kb on - strand, within gidBat 0.645 kb on - strandat 0.651 kb on + strandat 0.664 kb on - strandat 1.092 kb on - strand, within Shewana3_0002at 1.404 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction outer cut, LB soft agar motility assay
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148 + gidB Shewana3_0001 0.18 +1.6
279 - gidB Shewana3_0001 0.39 -2.4
331 - gidB Shewana3_0001 0.47 -3.4
377 + gidB Shewana3_0001 0.54 -2.3
385 - gidB Shewana3_0001 0.56 -2.3
385 - gidB Shewana3_0001 0.56 -1.4
439 - gidB Shewana3_0001 0.64 -4.3
479 + gidB Shewana3_0001 0.71 -4.5
479 + gidB Shewana3_0001 0.71 -3.5
479 + gidB Shewana3_0001 0.71 -2.4
479 + gidB Shewana3_0001 0.71 -2.7
479 + gidB Shewana3_0001 0.71 -3.4
487 - gidB Shewana3_0001 0.72 -4.2
487 - gidB Shewana3_0001 0.72 -2.3
516 - gidB Shewana3_0001 0.77 -2.6
547 - gidB Shewana3_0001 0.82 -2.7
645 - -4.7
651 + -3.6
664 - -3.4
1,092 - Shewana3_0002 0.17 -4.1
1,404 + +0.6

Or see this region's nucleotide sequence