Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_613 and Pf1N1B4_614 are separated by 446 nucleotides Pf1N1B4_614 and Pf1N1B4_615 are separated by 35 nucleotides
Pf1N1B4_613 - Arginine/ornithine antiporter ArcD, at 707,879 to 709,306
_613
Pf1N1B4_614 - Arginine/ornithine antiporter ArcD, at 709,753 to 711,180
_614
Pf1N1B4_615 - Arginine deiminase (EC 3.5.3.6), at 711,216 to 712,472
_615
Position (kb) 709
710
711
712 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.054 kb on - strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand, within Pf1N1B4_613 at 709.083 kb on + strand at 709.083 kb on + strand at 709.083 kb on + strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand, within Pf1N1B4_613 at 709.084 kb on - strand at 709.084 kb on - strand, within Pf1N1B4_613 at 709.335 kb on - strand at 709.374 kb on - strand at 709.385 kb on - strand at 709.422 kb on - strand at 709.425 kb on + strand at 709.426 kb on - strand at 709.473 kb on + strand at 709.473 kb on + strand at 709.474 kb on - strand at 709.529 kb on + strand at 709.540 kb on + strand at 709.541 kb on - strand at 709.541 kb on - strand at 709.643 kb on + strand at 709.643 kb on + strand at 709.652 kb on - strand at 709.882 kb on - strand at 709.976 kb on - strand, within Pf1N1B4_614 at 710.005 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand, within Pf1N1B4_614 at 710.065 kb on + strand at 710.816 kb on - strand, within Pf1N1B4_614 at 710.830 kb on + strand, within Pf1N1B4_614 at 710.861 kb on - strand, within Pf1N1B4_614 at 710.911 kb on + strand, within Pf1N1B4_614 at 710.912 kb on - strand at 710.912 kb on - strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.971 kb on + strand, within Pf1N1B4_614 at 710.990 kb on - strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.031 kb on + strand, within Pf1N1B4_614 at 711.123 kb on - strand at 711.123 kb on - strand at 711.214 kb on + strand at 711.237 kb on + strand at 711.756 kb on + strand, within Pf1N1B4_615 at 711.757 kb on - strand, within Pf1N1B4_615
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 709,054 - Pf1N1B4_613 0.82 +1.5 709,054 - Pf1N1B4_613 0.82 +2.5 709,054 - Pf1N1B4_613 0.82 -0.7 709,054 - Pf1N1B4_613 0.82 +0.9 709,083 + Pf1N1B4_613 0.84 -0.1 709,083 + Pf1N1B4_613 0.84 +0.9 709,083 + +1.5 709,083 + -1.1 709,083 + Pf1N1B4_613 0.84 -0.1 709,084 - Pf1N1B4_613 0.84 -0.7 709,084 - Pf1N1B4_613 0.84 -0.3 709,084 - Pf1N1B4_613 0.84 +0.3 709,084 - Pf1N1B4_613 0.84 -0.4 709,084 - Pf1N1B4_613 0.84 +0.5 709,084 - +0.5 709,084 - Pf1N1B4_613 0.84 -0.1 709,335 - -1.1 709,374 - -1.7 709,385 - -1.0 709,422 - -1.7 709,425 + -0.5 709,426 - -0.6 709,473 + +1.5 709,473 + -0.5 709,474 - -1.3 709,529 + -0.5 709,540 + +0.3 709,541 - -1.1 709,541 - -0.8 709,643 + -0.7 709,643 + -1.1 709,652 - +0.6 709,882 - -0.1 709,976 - Pf1N1B4_614 0.16 +1.5 710,005 + Pf1N1B4_614 0.18 -0.8 710,065 + Pf1N1B4_614 0.22 -0.7 710,065 + +0.9 710,816 - Pf1N1B4_614 0.74 -2.1 710,830 + Pf1N1B4_614 0.75 -0.4 710,861 - Pf1N1B4_614 0.78 +0.9 710,911 + Pf1N1B4_614 0.81 -3.3 710,912 - -0.1 710,912 - Pf1N1B4_614 0.81 -1.1 710,971 + Pf1N1B4_614 0.85 -1.1 710,971 + Pf1N1B4_614 0.85 -2.9 710,990 - Pf1N1B4_614 0.87 +0.9 711,031 + Pf1N1B4_614 0.89 -0.1 711,031 + Pf1N1B4_614 0.89 -1.1 711,123 - -0.7 711,123 - +0.9 711,214 + +1.5 711,237 + -1.7 711,756 + Pf1N1B4_615 0.43 -0.3 711,757 - Pf1N1B4_615 0.43 -2.1
Or see this region's nucleotide sequence