Experiment: Carnitine Hydrochloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5693 and Pf1N1B4_5694 are separated by 13 nucleotides Pf1N1B4_5694 and Pf1N1B4_5695 are separated by 20 nucleotides Pf1N1B4_5695 and Pf1N1B4_5696 are separated by 160 nucleotides Pf1N1B4_5696 and Pf1N1B4_5697 are separated by 360 nucleotides
Pf1N1B4_5693 - Gluconate dehydratase (EC 4.2.1.39), at 131,440 to 132,624
_5693
Pf1N1B4_5694 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 132,638 to 133,438
_5694
Pf1N1B4_5695 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 133,459 to 134,892
_5695
Pf1N1B4_5696 - hypothetical protein, at 135,053 to 135,181
_5696
Pf1N1B4_5697 - putative exported protein, at 135,542 to 136,108
_5697
Position (kb)
133
134
135 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 132.613 kb on + strand at 133.156 kb on + strand, within Pf1N1B4_5694 at 133.433 kb on + strand at 133.434 kb on - strand at 133.783 kb on + strand, within Pf1N1B4_5695 at 133.792 kb on + strand, within Pf1N1B4_5695 at 133.792 kb on + strand, within Pf1N1B4_5695 at 133.793 kb on - strand, within Pf1N1B4_5695 at 133.793 kb on - strand, within Pf1N1B4_5695 at 133.793 kb on - strand at 134.323 kb on + strand, within Pf1N1B4_5695 at 134.324 kb on - strand, within Pf1N1B4_5695 at 134.324 kb on - strand, within Pf1N1B4_5695 at 134.699 kb on - strand, within Pf1N1B4_5695 at 134.758 kb on + strand at 134.857 kb on + strand at 134.858 kb on - strand at 134.878 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.959 kb on + strand at 134.960 kb on - strand at 134.960 kb on - strand at 134.960 kb on - strand at 134.988 kb on + strand at 134.989 kb on - strand at 134.989 kb on - strand at 134.989 kb on - strand at 134.990 kb on + strand at 135.451 kb on + strand at 135.471 kb on + strand at 135.472 kb on - strand at 135.472 kb on - strand at 135.525 kb on + strand at 135.526 kb on - strand at 135.526 kb on - strand at 135.615 kb on + strand, within Pf1N1B4_5697 at 135.615 kb on + strand at 135.616 kb on - strand, within Pf1N1B4_5697 at 135.684 kb on - strand, within Pf1N1B4_5697 at 135.809 kb on + strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697 at 135.812 kb on - strand, within Pf1N1B4_5697
Per-strain Table
Position Strand Gene LocusTag Fraction Carnitine Hydrochloride (N) remove 132,613 + -0.5 133,156 + Pf1N1B4_5694 0.65 -1.4 133,433 + -1.3 133,434 - +2.5 133,783 + Pf1N1B4_5695 0.23 +0.7 133,792 + Pf1N1B4_5695 0.23 -0.9 133,792 + Pf1N1B4_5695 0.23 -1.9 133,793 - Pf1N1B4_5695 0.23 +0.9 133,793 - Pf1N1B4_5695 0.23 -1.5 133,793 - -0.3 134,323 + Pf1N1B4_5695 0.60 -0.3 134,324 - Pf1N1B4_5695 0.60 -0.3 134,324 - Pf1N1B4_5695 0.60 -1.6 134,699 - Pf1N1B4_5695 0.86 +0.0 134,758 + -2.3 134,857 + -0.3 134,858 - +0.7 134,878 + -1.3 134,959 + +1.3 134,959 + +0.3 134,959 + +0.1 134,959 + -0.3 134,959 + +0.7 134,959 + -1.6 134,960 - +0.5 134,960 - +1.7 134,960 - -0.7 134,988 + -0.6 134,989 - -0.3 134,989 - -0.3 134,989 - +0.3 134,990 + -1.9 135,451 + +0.5 135,471 + +1.3 135,472 - -1.3 135,472 - +0.1 135,525 + +1.3 135,526 - +0.3 135,526 - +0.7 135,615 + Pf1N1B4_5697 0.13 -0.3 135,615 + -1.3 135,616 - Pf1N1B4_5697 0.13 -0.3 135,684 - Pf1N1B4_5697 0.25 -0.3 135,809 + Pf1N1B4_5697 0.47 -0.1 135,812 - Pf1N1B4_5697 0.48 -0.3 135,812 - Pf1N1B4_5697 0.48 -0.3 135,812 - Pf1N1B4_5697 0.48 +0.2 135,812 - Pf1N1B4_5697 0.48 +2.3 135,812 - Pf1N1B4_5697 0.48 -1.2 135,812 - Pf1N1B4_5697 0.48 +2.0
Or see this region's nucleotide sequence